Disease/Experimental Model

Development of a 3D simulator for training the mouse in utero electroporation

In utero electroporation (IUE) requires high-level training in microinjection through the mouse uterine wall into the lateral ventricle of the mouse brain. Training for IUE is currently being performed in live mice as no artificial models allow simulations yet. This...

Year Published: 2022Topics: Disease/Experimental Model, Drug/Substance Administration, ReproductionAnimal Type: Mouse, Rodent

Citation: Nuber, M., Gonzalez-Uarquin, F., Neufurth, M. et al. 2022. Development of a 3D simulator for training the mouse in utero electroporation. PLOS ONE 17(12), e0279004.

Probing microstructural changes in muscles of leptin-deficient zebrafish by non-invasive ex-vivo magnetic resonance microimaging

Leptin is a hormone that plays a key role in controlling food intake and energy homeostasis. Skeletal muscle is an important target for leptin and recent studies have shown that leptin deficiency may lead to muscular atrophy. However, leptin deficiency-induced...

Year Published: 2023Topics: Disease/Experimental Model, Imaging & Electrophysiological RecordingAnimal Type: Fish, Zebrafish

Citation: Eeza, M. N. H., Singer, R., Ding, Y. et al. 2023. Probing microstructural changes in muscles of leptin-deficient zebrafish by non-invasive ex-vivo magnetic resonance microimaging. PLOS ONE 18(4), e0284215.

Novel protocol to establish the myocardial infarction model in rats using a combination of medetomidine-midazolam-butorphanol (MMB) and atipamezole

Myocardial infarction (MI) is one of the most common cardiac problems causing deaths in humans. Previously validated anesthetic agents used in MI model establishment are currently controversial with severe restrictions because of ethical concerns. The combination between medetomidine, midazolam, and...

Year Published: 2022Topics: Anesthesia & Sedation, Disease/Experimental ModelAnimal Type: Rat, Rodent

Citation: Farag, A., Mandour, A. S., Hamabe, L. et al. 2022. Novel protocol to establish the myocardial infarction model in rats using a combination of medetomidine-midazolam-butorphanol (MMB) and atipamezole. Frontiers in Veterinary Science 9, 1064836.

A review on experimental surgical models and anesthetic protocols of heart failure in rats

Heart failure (HF) is a serious health and economic burden worldwide, and its prevalence is continuously increasing. Current medications effectively moderate the progression of symptoms, and there is a need for novel preventative and reparative treatments. The development of novel...

Year Published: 2023Topics: Anesthesia & Sedation, Disease/Experimental ModelAnimal Type: Rat, Rodent

Citation: Farag, A., Mandour, A. S., Hendawy, H. et al. 2023. A review on experimental surgical models and anesthetic protocols of heart failure in rats. Frontiers in Veterinary Science 10, 1103229.

Tetra-primer amplification-refractory mutationsystem (ARMS)—PCR for genotyping mouse leptin gene mutation

Due to spontaneous deficiency in leptin, ob/ob mice are one of the most commonly used experimental animal models in diabetes research. In this study, we reported a quick and easy-to-conduct genotyping method using tetra-primer amplification refractory mutation system-polymerase chain reaction...

Year Published: 2022Topics: Disease/Experimental Model, Identification MethodAnimal Type: Mouse, Rodent

Citation: Chen, J., Xu, X., Dalhaimer, P. et al. 2022. Tetra-primer amplification-refractory mutationsystem (ARMS)—PCR for genotyping mouse leptin gene mutation. Animals 12(19), 2680.

Impact of partial bile duct ligation with or without repeated magnetic resonance imaging examinations in mice

Partial bile duct ligation (pBDL) is considered a well-tolerated cholestatic model. Magnetic resonance imaging (MRI) is one of the most widely used tools in noninvasive imaging. However, no systematic studies have reported the possible effects of repeated MRI assessments in...

Year Published: 2022Topics: Disease/Experimental Model, Imaging & Electrophysiological RecordingAnimal Type: Mouse, Rodent

Citation: Chen, T., Zhou, Z.-Y., Liu, J.-Y. et al. 2022. Impact of partial bile duct ligation with or without repeated magnetic resonance imaging examinations in mice. Scientific Reports 12(1), 21014.

Animal welfare considerations when conducting OECD test guideline inhalation and toxicokinetic studies for nanomaterials

The OECD test guidelines for animal experiments play an important role in evaluating the chemical hazards. Animal tests performed using OECD guidelines, especially when the good laboratory practice (GLP) principle is applied, reduce the duplication of toxicity testing and ensure...

Year Published: 2022Topics: Disease/Experimental ModelAnimal Type: All/General, Rat, Rodent

Citation: Chung, Y. H., Gulumian, M., Pleus, R. C. et al. 2022. Animal welfare considerations when conducting OECD test guideline inhalation and toxicokinetic studies for nanomaterials. Animals 12(23), 3305.

Enriched environment causes epigenetic changes in hippocampus and improves long-term cognitive function in sepsis

Sepsis is defined as a life-threatening organ dysfunction caused by an inappropriate host response to infection. The presence of oxidative stress and inflammatory mediators in sepsis leads to dysregulated gene expression, leading to a hyperinflammatory response. Environmental conditions play an...

Year Published: 2022Topics: Disease/Experimental Model, Environmental EnrichmentAnimal Type: Rat, Rodent

Citation: Córneo, E., Michels, M., Abatti, M. et al. 2022. Enriched environment causes epigenetic changes in hippocampus and improves long-term cognitive function in sepsis. Scientific Reports 12(1), 11529.

Longitudinal home-cage automated assessment of climbing behavior shows sexual dimorphism and aging-related decrease in C57BL/6J healthy mice and allows early detection of motor impairment in the N171-82Q mouse model of Huntington’s disease

Monitoring the activity of mice within their home cage is proving to be a powerful tool for revealing subtle and early-onset phenotypes in mouse models. Video-tracking, in particular, lends itself to automated machine-learning technologies that have the potential to improve...

Year Published: 2023Topics: Behavioral & Cognitive Testing, Disease/Experimental Model, HandlingAnimal Type: Mouse, Rodent

Citation: Bains, R. S., Forrest, H., Sillito, R. R. et al. 2023. Longitudinal home-cage automated assessment of climbing behavior shows sexual dimorphism and aging-related decrease in C57BL/6J healthy mice and allows early detection of motor impairment in the N171-82Q mouse model of Huntington's disease. Frontiers in Behavioral Neuroscience 17, 1148172.

Animal welfare assessment after severe traumatic brain injury in rats

Severe traumatic brain injury (TBI) is a multifactorial injury process involving respiratory, cardiovascular and immune functions in addition to the brain. Thus, live animal models are needed to study the molecular, cellular and systemic mechanisms of TBI. The ethical use...

Year Published: 2022Topics: Disease/Experimental Model, Welfare AssessmentAnimal Type: Rat, Rodent

Citation: Allioux, C., Achaintre, L., Cheataini. F. et al. 2022. Animal welfare assessment after severe traumatic brain injury in rats. Laboratory Animals 56(6), 528-539.