Disease/Experimental Model

Dos and don’ts in large animal models of aortic insufficiency

Aortic insufficiency caused by paravalvular leakage (PVL) is one of the most feared complications following transcatheter aortic valve replacement (TAVI) in patients. Domestic pigs (Sus scrofa domestica) are a popular large animal model to study such conditions and develop novel...

Year Published: 2022Topics: Disease/Experimental ModelAnimal Type: Pig

Citation: Weisskopf, M., Glaus, L., Trimmel, N. E. et al. 2022. Dos and don'ts in large animal models of aortic insufficiency. Frontiers in Veterinary Science 9, 949410.

A buprenorphine depot formulation provides effective sustained post-surgical analgesia for 72 h in mouse femoral fracture models

Adequate pain management is essential for ethical and scientific reasons in animal experiments and should completely cover the period of expected pain without the need for frequent re-application. However, current depot formulations of Buprenorphine are only available in the USA...

Year Published: 2023Topics: Analgesia, Disease/Experimental ModelAnimal Type: Mouse, Rodent

Citation: Wolter, A., Bucher, C. H., Kurmies, S. et al. 2023. A buprenorphine depot formulation provides effective sustained post-surgical analgesia for 72 h in mouse femoral fracture models. Scientific Reports 13(1), 3824.

Ultrasound guided surgery as a refinement tool in oncology research

Refinement is one of the ethical pillars of the use of animals in research. Ultrasonography is currently used in human medicine as a surgical tool for guided biopsies and this idea can be applied to preclinical research thanks to the...

Year Published: 2022Topics: Disease/Experimental Model, Drug/Substance Administration, Imaging & Electrophysiological Recording, Surgery & Post-OpAnimal Type: All/General

Citation: Serrano, C., Antonio, J. 2022. Ultrasound guided surgery as a refinement tool in oncology research. Animals 12(23), 3445.

Studying and analyzing humane endpoints in the fructose-fed and streptozotocin-injected rat model of diabetes

This work aimed to define a humane endpoint scoring system able to objectively identify signs of animal suffering in a rat model of type 2 diabetes. Sprague-Dawley male rats were divided into control and induced group. The induced animals drink...

Year Published: 2023Topics: Disease/Experimental Model, Humane Endpoint, Welfare AssessmentAnimal Type: Rat, Rodent

Citation: Silva-Reis, R., Faustino-Rocha, A. I., Silva, J. et al. 2023. Studying and analyzing humane endpoints in the fructose-fed and streptozotocin-injected rat model of diabetes. Animals 13(8), 1397.

Longitudinal studies on Alzheimer disease mouse models with multiple tracer PET/CT: Application of reduction and refinement principles in daily practice to safeguard animal welfare during progressive aging

Longitudinal studies on mouse models related to Alzheimer disease (AD) pathology play an important role in the investigation of therapeutic targets to help pharmaceutical research in the development of new drugs and in the attempt of an early diagnosis that...

Year Published: 2023Topics: Disease/Experimental Model, Husbandry & Management, Imaging & Electrophysiological RecordingAnimal Type: Mouse, Rodent

Citation: Palumbo, G., Kunze, L. H., Oos, R. et al. 2023. Longitudinal studies on Alzheimer disease mouse models with multiple tracer PET/CT: Application of reduction and refinement principles in daily practice to safeguard animal welfare during progressive aging. Animals 13(11), 1812.

Are currently selected laboratory animals useful in the research of how female hormones influence orthodontic biomechanics?

Animal testing was and remains the only method of introducing a certain treatment and medical procedure on humans. On the other hand, animals have their rights resulting from applicable legal acts, including Directive 2010/63/EU and, indirectly, the World Medical Association...

Year Published: 2023Topics: Disease/Experimental Model, Regulations & Ethical ReviewAnimal Type: All/General, Cat, Rabbit, Rat, Rodent

Citation: Peruga, M., Kawala, B., Sarul, M. et al. 2023. Are currently selected laboratory animals useful in the research of how female hormones influence orthodontic biomechanics? Animals 13(4), 629.

Validation of a 3D perfused cell culture platform as a tool for humanised preclinical drug testing in breast cancer using established cell lines and patient-derived tissues

3D cell culture models of cancer are currently being developed to recapitulate in vivo physiological conditions and to assess therapeutic responses. However, most models failed to incorporate the biochemical and biophysical stimuli from fluid flow. In this study, a three-dimensional...

Year Published: 2023Topics: Disease/Experimental ModelAnimal Type: All/General

Citation: Liu, P., Roberts, S., Shoemaker, J. T. et al. 2023. Validation of a 3D perfused cell culture platform as a tool for humanised preclinical drug testing in breast cancer using established cell lines and patient-derived tissues. PLOS ONE 18(3), e0283044.

Development of a 3D simulator for training the mouse in utero electroporation

In utero electroporation (IUE) requires high-level training in microinjection through the mouse uterine wall into the lateral ventricle of the mouse brain. Training for IUE is currently being performed in live mice as no artificial models allow simulations yet. This...

Year Published: 2022Topics: Disease/Experimental Model, Drug/Substance Administration, ReproductionAnimal Type: Mouse, Rodent

Citation: Nuber, M., Gonzalez-Uarquin, F., Neufurth, M. et al. 2022. Development of a 3D simulator for training the mouse in utero electroporation. PLOS ONE 17(12), e0279004.

Probing microstructural changes in muscles of leptin-deficient zebrafish by non-invasive ex-vivo magnetic resonance microimaging

Leptin is a hormone that plays a key role in controlling food intake and energy homeostasis. Skeletal muscle is an important target for leptin and recent studies have shown that leptin deficiency may lead to muscular atrophy. However, leptin deficiency-induced...

Year Published: 2023Topics: Disease/Experimental Model, Imaging & Electrophysiological RecordingAnimal Type: Fish, Zebrafish

Citation: Eeza, M. N. H., Singer, R., Ding, Y. et al. 2023. Probing microstructural changes in muscles of leptin-deficient zebrafish by non-invasive ex-vivo magnetic resonance microimaging. PLOS ONE 18(4), e0284215.

Novel protocol to establish the myocardial infarction model in rats using a combination of medetomidine-midazolam-butorphanol (MMB) and atipamezole

Myocardial infarction (MI) is one of the most common cardiac problems causing deaths in humans. Previously validated anesthetic agents used in MI model establishment are currently controversial with severe restrictions because of ethical concerns. The combination between medetomidine, midazolam, and...

Year Published: 2022Topics: Anesthesia & Sedation, Disease/Experimental ModelAnimal Type: Rat, Rodent

Citation: Farag, A., Mandour, A. S., Hamabe, L. et al. 2022. Novel protocol to establish the myocardial infarction model in rats using a combination of medetomidine-midazolam-butorphanol (MMB) and atipamezole. Frontiers in Veterinary Science 9, 1064836.