Disease/Experimental Model

Improving rat welfare through the development of a peribulbar anaesthesia technique for ophthalmic procedures: A preliminary study

Rats are a commonly used animal model for the study of the pathogenesis and novel treatments of glaucoma, which is induced experimentally using invasive, painful procedures. Peribulbar anaesthesia (PBA) is frequently used in people and domestic animals prior to ophthalmic...

Year Published: 2024Topics: Anesthesia & Sedation, Disease/Experimental ModelAnimal Type: Rat, Rodent

Citation: Eliav, A., Ofri, R., Brust, K. et al. 2024. Improving rat welfare through the development of a peribulbar anaesthesia technique for ophthalmic procedures: A preliminary study. Laboratory Animals 58(2), 116–126.

Tamoxifen exacerbates morbidity and mortality in male mice receiving medetomidine anaesthesia

Tamoxifen-induced CreER-LoxP recombination is often used to induce spatiotemporally controlled gene deletion in genetically modified mice. Prior work has shown that tamoxifen and tamoxifen-induced CreER activation can have off-target effects that should be controlled. However, it has not yet been...

Year Published: 2023Topics: Anesthesia & Sedation, Disease/Experimental ModelAnimal Type: Mouse, Rodent

Citation: Rashbrook, V. S., Denti, L., Ruhrberg, C. 2023. Tamoxifen exacerbates morbidity and mortality in male mice receiving medetomidine anaesthesia. Animal Welfare 32, e78.

Refining the welfare of immunocompromised mice receiving carbon tetrachloride (CCI4) to induce liver fibrosis

Our method provides evidence that we can alter CCI4 dosing and implement recovery techniques to enhance Animal Welfare, whilst maintaining sufficient levels of fibrosis in the liver.

Year Published: 2023Topics: Disease/Experimental ModelAnimal Type: Mouse, Rodent

Citation: Walczak-Gillies, Matthews, O., Fyfe, A. et al. 2023. Refining the welfare of immunocompromised mice receiving carbon tetrachloride (CCI4) to induce liver fibrosis. Animal Technology and Welfare 22(3), 237–239.

A minimally invasive bronchoscopic approach for direct delivery to murine airways and application to models of pulmonary infection

The laboratory mouse is used extensively for human disease modeling and preclinical therapeutic testing for efficacy, biodistribution, and toxicity. The variety of murine models available, and the ability to create new ones, eclipses all other species, but the size of...

Year Published: 2023Topics: Cannulation, Catheterization, & Intubation, Disease/Experimental ModelAnimal Type: Mouse, Rodent

Citation: Schelde, K., Rosenjack, J., Sonneborn, C. et al. 2023. A minimally invasive bronchoscopic approach for direct delivery to murine airways and application to models of pulmonary infection. Laboratory Animals 57(6), 611–622.

Evaluation of the effect of tramadol, paracetamol and metamizole on the severity of experimental colitis

Application of dextran sodium sulfate (DSS) is often used to induce experimental colitis. Current state of the art is to refrain from the use of analgesics due to their possible interaction with the model. However, the use of analgesics would...

Year Published: 2023Topics: Analgesia, Disease/Experimental ModelAnimal Type: Mouse, Rodent

Citation: Spalinger, M., Schwarzfischer, M., Niechcial, A. et al. 2023. Evaluation of the effect of tramadol, paracetamol and metamizole on the severity of experimental colitis. Laboratory Animals 57(5), 529–540.

An optimized method for intratracheal instillation in mice

Non-invasive intratracheal instillation is an important method for direct exposure of the respiratory tract which is commonly used in toxicology, environmental science, and other research fields. However, there is no standard operating process for non-invasive intratracheal instillation. To keep the...

Year Published: 2022Topics: Disease/Experimental Model, Drug/Substance AdministrationAnimal Type: Mouse, Rodent

Citation: Zeng, Y., Jin, H., Wang, J. et al. 2022. An optimized method for intratracheal instillation in mice. Journal of Pharmacological and Toxicological Methods 118, 107230.

Comparing three formulations of buprenorphine in an incisional pain model in mice

This study compared the therapeutic effects in mice of 3 different formulations of buprenorphine. These formulations were standard buprenorphine hydrochloride (Bup-HCL) and 2 different extended-release buprenorphine formulations (Bup-ER and Ethiqa-XR [Bup-XR]). Drugs were evaluated based on their ability to attenuate...

Year Published: 2023Topics: Analgesia, Disease/Experimental ModelAnimal Type: Mouse, Rodent

Citation: Lopez-Echeverria, G., Alamaw, E., Gorman, G. et al. 2023. Comparing three formulations of buprenorphine in an incisional pain model in mice. JAALAS 62(6), 531–537.

Effects of extended-release buprenorphine on mouse models of influenza

Mice are widely used as small animal models for influenza infection and immunization studies because of their susceptibility to many strains of influenza, obvious clinical signs of infection, and ease of handling. Analgesia is rarely used in such studies even...

Year Published: 2023Topics: Analgesia, Disease/Experimental ModelAnimal Type: Mouse, Rodent

Citation: Brake, M. E., Russ, B. P., Gansebom, S. et al. 2023. Effects of extended-release buprenorphine on mouse models of influenza. Comparative Medicine 73(6), 466–473.

Which form of environmental enrichment is most effective in rodent models of autism?

Environmental enrichment (EE) is known to produce experience-dependent changes in the brains and behaviors of rodents, and it has therefore been widely used to study neurodevelopmental disorders, including autism. Current studies show significant protocol variation, such as the presence of...

Year Published: 2023Topics: Disease/Experimental Model, Environmental EnrichmentAnimal Type: Mouse, Rat, Rodent

Citation: Caires, C. R. S., Bossolani-Martins, A. L. 2023. Which form of environmental enrichment is most effective in rodent models of autism? Behavioural Processes 211, 104915.

A novel scoring system for humane endpoints in mice with cecal ligation and puncture-induced sepsis

Animal-based research is essential to the study of sepsis pathophysiology, diagnostics, and therapeutics. However, animal models of sepsis are often associated with high mortality because of the difficulty in predicting imminent death based on premortem assessment of the animals. The...

Year Published: 2023Topics: Disease/Experimental Model, Humane EndpointAnimal Type: Mouse, Rodent

Citation: Ferguson, L. T., Rashied, A. A., Liang, Z. et al. 2023. A novel scoring system for humane endpoints in mice with cecal ligation and puncture-induced sepsis. Comparative Medicine 73(6), 446–460.