Biological Sampling & Physiological Measurement

The cotton rat – a new challenge

This poster describes how the Animal Technologists at Envigo adapted their current practices and procedures for a new species at their laboratory: the cotton rat. The paper describes cotton rat biology and behaviour; housing; environmental enrichment; health; dosing and blood...

Year Published: 2020Topics: Anesthesia & Sedation, Biological Sampling & Physiological Measurement, Drug/Substance Administration, Environmental Enrichment, Euthanasia, Housing, Husbandry & Management, Natural BehaviorAnimal Type: Rat, Rodent

Citation: Ballantine, A., Rodgers, D., Watts, C. et al. 2020. The cotton rat – a new challenge. Animal Technology and Welfare 19(1), 92-95.

The impact of common recovery blood sampling methods, in mice (Mus musculus), on well-being and sample quality: A systematic review

Blood sampling is often performed in laboratory mice. Sampling techniques have the potential to cause pain, distress and impact on lifetime cumulative experience. In spite of institutions commonly providing guidance to researchers on these methods, and the existence of published...

Year Published: 2020Topics: Biological Sampling & Physiological MeasurementAnimal Type: Mouse, Rodent

Citation: Whittaker, A. L., Barker, T. H. 2020. The impact of common recovery blood sampling methods, in mice (Mus musculus), on well-being and sample quality: A systematic review. Animals 10(6), 989.

Assessment of hematologic and corticosterone response in free-living eastern box turtles (Terrapene carolina carolina) at capture and after handling

Hematology is a common tool for wildlife health assessments. Manual leukocyte counts are required in reptiles, however, disagreement between quantification methods has been observed in some chelonians. This study determined agreement between two methods of leukocyte quantification, eosinophilic leukopet, and...

Year Published: 2020Topics: Biological Sampling & Physiological Measurement, HandlingAnimal Type: Reptile, Turtle & Tortoise

Citation: Boers, K. L., Allender, M. C., Novak, L. J. et al. 2020. Assessment of hematologic and corticosterone response in free-living eastern box turtles (Terrapene carolina carolina) at capture and after handling. Zoo Biology 39(1), 13-22.

Assessment of non-contact infrared thermometer measurement sites in birds

The standard method of obtaining body temperature in a bird can be a stressful event, making routine evaluations challenging. Twenty-eight privately owned birds in good health were enrolled in the study to compare digital and infrared (IR) temperature readings. Digital...

Year Published: 2020Topics: Biological Sampling & Physiological MeasurementAnimal Type: Bird, Chicken, Finch, Other Bird, Parrot

Citation: Anderson, J., Kaplan-Stein, S., Adolph, S. et al. 2020. Assessment of non-contact infrared thermometer measurement sites in birds. Journal of Applied Animal Welfare Science 23(2), 131-139.

Automated collection and analysis of infrared thermograms for measuring eye and cheek temperatures in calves

As the reliance upon automated systems in the livestock industry increases, technologies need to be developed which can be incorporated into these systems to monitor animal health and welfare. Infrared thermography (IRT) is one such technology that has been used...

Year Published: 2020Topics: Biological Sampling & Physiological Measurement, Welfare AssessmentAnimal Type: Cattle

Citation: Lowe, G., McCane, B., Sutherland, M. et al. 2020. Automated collection and analysis of infrared thermograms for measuring eye and cheek temperatures in calves. Animals 10(2), 292.

Best practices for non-lethal blood sampling of fish via the caudal vasculature

Blood sampling through the caudal vasculature is a widely used technique in fish biology for investigating organismal health and physiology. In live fishes, it can provide a quick, easy and relatively non‐invasive method for obtaining a blood sample (cf ....

Year Published: 2020Topics: Biological Sampling & Physiological MeasurementAnimal Type: Fish, Other Fish, Salmon, Tilapia, Trout, Zebrafish

Citation: Lawrence, M. J., Raby, G. D., Teffer, A. K. et al. 2020. Best practices for non-lethal blood sampling of fish via the caudal vasculature. Journal of Fish Biology 97(1), 4–15.

Continuous and non-invasive thermography of mouse skin accurately describes core body temperature patterns, but not absolute core temperature

Body temperature is an important physiological parameter in many studies of laboratory mice. Continuous assessment of body temperature has traditionally required surgical implantation of a telemeter, but this invasive procedure adversely impacts animal welfare. Near-infrared thermography provides a non-invasive alternative...

Year Published: 2020Topics: Biological Sampling & Physiological MeasurementAnimal Type: Mouse, Rodent

Citation: van der Vinne, V., Pothecary, C. A., Wilcox, S. L. et al. 2020. Continuous and non-invasive thermography of mouse skin accurately describes core body temperature patterns, but not absolute core temperature. Scientific Reports 10(1), 20680.

Evaluation of an acute osmotic stress in European sea bass via skin mucus biomarkers

European sea bass is a marine teleost which can inhabit a broad range of environmental salinities. So far, no research has studied the physiological response of this fish to salinity challenges using modifications in skin mucus as a potential biological...

Year Published: 2020Topics: Biological Sampling & Physiological Measurement, Husbandry & Management, Welfare AssessmentAnimal Type: Fish, Other Fish

Citation: Ordóñez-Grande, B., Guerreiro, P. M., Sanahuja, I. et al. 2020. Evaluation of an acute osmotic stress in European sea bass via skin mucus biomarkers. Animals 10(9), 1546.

Evaluation of infrared thermography as a non-invasive method of measuring the autonomic nervous response in sheep

Eye temperature measured using infrared thermography (IRT) can be used as a non-invasive measure of autonomic nervous system (ANS) activity in cattle. The objective of this study was to evaluate if changes in eye temperature (measured using IRT) can be...

Year Published: 2020Topics: Biological Sampling & Physiological Measurement, Welfare AssessmentAnimal Type: Sheep

Citation: Sutherland, M. A., Worth, G. M., Dowling, S. K. et al. 2020. Evaluation of infrared thermography as a non-invasive method of measuring the autonomic nervous response in sheep. PLOS ONE 15(5): e0233558.

Four- and six-hour urinary albumin excretion is a valuable alternative to 24-h urinary albumin excretion in male db/db mice

In mouse (Mus musculus) models of diabetic nephropathy (DN), one of the most important read-outs is the 24-h urinary albumin excretion (UAE). The 24-h urine collection is usually performed by single housing mice in metabolic cages on wire mesh without...

Year Published: 2020Topics: Biological Sampling & Physiological Measurement, Disease/Experimental ModelAnimal Type: Mouse, Rodent

Citation: Nøgaard, S. A., Sand, F. W., Sørensen, D. B. et al. 2020. Four- and six-hour urinary albumin excretion is a valuable alternative to 24-h urinary albumin excretion in male db/db mice. Animal Welfare 29(2), 143-149.