Behavioral & Cognitive Testing

Habituation with apparatus and group testing improves assessment of fish preferences

Preference tests are commonly used to assess fish behavior and cognition in several research fields. This study aimed to investigate how fish perform in a preference test involving extended habituation to the apparatus, which was expected to reduce stress. We...

Year Published: 2025Topics: Behavioral & Cognitive TestingAnimal Type: Fish, Other Fish

Citation: Varracchio, C., Paci, F. P., Bertolucci, C. et al. 2025. Habituation with apparatus and group testing improves assessment of fish preferences. Journal of Fish Biology 107(4), 1149–1158.

The role of line-of-sight in operant experiments using food reinforcers in guinea pigs

The guinea pig (Cavia porcellus) is a species utilized as a model in laboratory science and is a popular companion animal. The study aimed to assess the role of line-of-sight with a food reinforcer in the ramp climbing behaviour of...

Year Published: 2025Topics: Behavioral & Cognitive TestingAnimal Type: Guinea Pig, Rodent

Citation: Cameron, K. E., Hoult, C., Walker, T. W. 2025. The role of line-of-sight in operant experiments using food reinforcers in guinea pigs. Behavioural Processes 228, 105189.

Exploring the impact of a brief positive experience on dogs’ performance and stress resilience during a learning task

Learning and stress resilience can be influenced by recent experiences. Research has traditionally focused on the effects of negative situations and stressors on subsequent learning and stress resilience, while knowledge is limited regarding the effects of positive experiences. We aimed...

Year Published: 2025Topics: Behavioral & Cognitive Testing, Human-Animal InteractionAnimal Type: Dog

Citation: Miller, J., Cavalli, C., Azadian, A. et al. 2025. Exploring the impact of a brief positive experience on dogs’ performance and stress resilience during a learning task. PLOS ONE 20(6), e0326368.

Opinion: Ethical challenges in depression research: The tail suspension test, the forced swim test, and alternative behavioral models

Behavioral tests in laboratory animals, particularly rodents, are considered vital for understanding the mechanisms underlying depression and evaluating potential treatments. However, ethical concerns regarding the use of traditional methods, such as the tail suspension test and the forced swimming test,...

Year Published: 2025Topics: Behavioral & Cognitive TestingAnimal Type: Mouse, Rat, Rodent

Citation: Mohseni, F., Rafaiee, R. 2025. Opinion: Ethical challenges in depression research: The tail suspension test, the forced swim test, and alternative behavioral models. Journal of the American Association for Laboratory Animal Science 64(3), 352–355.

Tracking ‘tails’: Refining motor activity monitoring in rats and mice (2025)

Motor activity monitoring is used in specialist regulatory toxicology studies to investigate test item related neurobehavioral effects. Introduction of a new video tracking software system which detects the centre point, tail base and nose tip of rodents to individually track...

Year Published: 2025Topics: Behavioral & Cognitive Testing, Biological Sampling & Physiological MeasurementAnimal Type: Mouse, Rat, Rodent

Citation: Reading, R. 2025. Tracking “tails”: Refining motor activity monitoring in rats and mice. Animal Technology and Welfare 24(1), 53–56.

A bespoke water T–maze apparatus and protocol: An optimized, reliable, and repeatable method for screening learning, memory, and executive functioning in laboratory mice

The Morris Water Maze (MWM) is the most commonly used assay for evaluating learning and memory in laboratory mice. Despite its widespread use, contemporary reviews have highlighted substantial methodological variation in experimental protocols and that the associated testing procedures are...

Year Published: 2024Topics: Behavioral & Cognitive TestingAnimal Type: Mouse, Rodent

Citation: Bailoo, J. D., Bergeson, S. E., Ponomarev, I. et al. 2024. A bespoke water T–maze apparatus and protocol: An optimized, reliable, and repeatable method for screening learning, memory, and executive functioning in laboratory mice. Frontiers in Behavioral Neuroscience 18.

Applying an eye tracking technique to gibbons: First study using scanpath measurements for visual stimuli

Compared to the abundance of research on cognition in various nonhuman primate species, studies of gibbons -- often called “the small apes” -- remain limited, despite the importance of gibbons for understanding evolutionary processes in humans and other apes. Over...

Year Published: 2024Topics: Behavioral & Cognitive TestingAnimal Type: Gibbon, Nonhuman Primate

Citation: Uchikoshi, M., Yu, L., Hattori, Y. 2024. Applying an eye tracking technique to gibbons: First study using scanpath measurements for visual stimuli. Behavioural Processes 221, 105080.

Determining the preferred liquid reward in adult C57BL/6 mice

Using food rewards to motivate mice is commonplace in behavioural research. Using a reward which is highly desirable is of benefit, as it can reduce the need to food restrict animals and can encourage higher levels of consumption, allowing for...

Year Published: 2023Topics: Behavioral & Cognitive Testing, Drug/Substance AdministrationAnimal Type: Mouse, Rodent

Citation: Miller, A. L., Leach, M. C. 2023. Determining the preferred liquid reward in adult C57BL/6 mice. Laboratory Animals 57(3), 332–335.

Using anticipatory behavior as preference test in the bottlenose dolphin (Tursiops truncatus)

The preference tests have made a great contribution to promoting animal welfare. However, they are not always easily applicable and have some criticalities. Recently the anticipatory behavior has been used to detect the animals' preference. In this study, we attempted...

Year Published: 2023Topics: Behavioral & Cognitive Testing, Welfare AssessmentAnimal Type: Marine Mammal

Citation: Bigiani, S., Pilenga, C. 2023. Using anticipatory behavior as preference test in the bottlenose dolphin (Tursiops truncatus). Journal of Applied Animal Welfare Science 26(2), 247–255.

A fast technique to induce and measure anticipatory behavior in bottlenose dolphins (Tursiops truncatus)

Anticipatory behavior describes the actions taken to prepare for an upcoming event. It is considered a promising tool for welfare assessment, but it is not fully applied in zoos and other facilities. This is probably due to the need to...

Year Published: 2024Topics: Behavioral & Cognitive Testing, Welfare AssessmentAnimal Type: Marine Mammal

Citation: Bigiani, S., Pilenga, C. 2024. A fast technique to induce and measure anticipatory behavior in bottlenose dolphins (Tursiops truncatus). Journal of Applied Animal Welfare Science 27(1), 137–149.