Publications

Rats move nesting materials to create different functional areas: Short report

Here we document how rats separate their living space into different functional regions. Five groups of four female Sprague Dawley rats were housed in caging systems that consisted of two standard cages connected by a tube. Both cages were provided...

Year Published: 2023Topics: Environmental Enrichment, HousingAnimal Type: Rat, Rodent

Citation: Amendola, L., Xu, N., Weary, D. M. 2023. Rats move nesting materials to create different functional areas: Short report. Laboratory Animals 57(1), 75–78.

Comparative study of tricaine methanesulfonate (MS-222) and eugenol as euthanasia agents in zebrafish (Danio rerio) as an experimental model

In experimental procedures inevitably leading to the sacrifice of animals, suitable measures should be taken to minimise their pain and suffering as much as possible, as well as to prevent any modification or masking in the experimental results obtained. An...

Year Published: 2023Topics: EuthanasiaAnimal Type: Fish, Zebrafish

Citation: Ayala-Soldado, N., Mora-Medina, R., Lora-Benítez, A. J. et al. 2023. Comparative study of tricaine methanesulfonate (MS-222) and eugenol as euthanasia agents in zebrafish (Danio rerio) as an experimental model. Laboratory Animals 57(3), 236–246.

Getting a handle on rat familiarization: The impact of handling protocols on classic tests of stress in Rattus norvegicus

Experimenter familiarization with laboratory rodents through handling prior to experimentation is an important practice in neurobehavioral research and is implicated in stress, study variability, and replicability. Unfortunately, different handling protocols have not been thoroughly examined. Determining optimal experimenter familiarization protocols...

Year Published: 2023Topics: HandlingAnimal Type: Rat, Rodent

Citation: Bigelow, L. J., Pope, E. K., MacDonald, D. S. et al. 2023. Getting a handle on rat familiarization: The impact of handling protocols on classic tests of stress in Rattus norvegicus. Laboratory Animals 57(3), 259–269.

Mouse isoflurane anesthesia using the drop method

Anesthesia with isoflurane prior to carbon dioxide euthanasia is recommended as a refinement, but vaporizer access can be limited. An alternative to vaporizers is the ‘drop' method, introducing a fixed volume of isoflurane into the induction chamber. Previous work suggests...

Year Published: 2023Topics: Anesthesia & Sedation, EuthanasiaAnimal Type: Mouse, Rodent

Citation: Bodnar, M. J., Ratuski, A. S., Weary, D. M. 2023. Mouse isoflurane anesthesia using the drop method. Laboratory Animals 57(6), 623–630.

Feasibility of pair-housing of rats after cranial implant surgery

Rat models employing cranial implants are increasingly employed to facilitate neural stimulation and recording in freely moving animals. Due to possible damage to wound, implant or attached devices, rats with cranial implants are traditionally housed singly, and little information is...

Year Published: 2023Topics: External Bodily Equipment, Social Housing & CompanionshipAnimal Type: Rat, Rodent

Citation: Evers, J., Sridhar, K., Lowery, M. 2023. Feasibility of pair-housing of rats after cranial implant surgery. Laboratory Animals 57(1), 69–74.

Determining the preferred liquid reward in adult C57BL/6 mice

Using food rewards to motivate mice is commonplace in behavioural research. Using a reward which is highly desirable is of benefit, as it can reduce the need to food restrict animals and can encourage higher levels of consumption, allowing for...

Year Published: 2023Topics: Behavioral & Cognitive Testing, Drug/Substance AdministrationAnimal Type: Mouse, Rodent

Citation: Miller, A. L., Leach, M. C. 2023. Determining the preferred liquid reward in adult C57BL/6 mice. Laboratory Animals 57(3), 332–335.

General and species-specific recommendations for minimal requirements for the use of cephalopods in scientific research

Here we list species-specific recommendations for housing, care and management of cephalopod molluscs employed for research purposes with the aim of contributing to the standardization of minimum requirements for establishments, care and accommodation of these animals in compliance with the...

Year Published: 2023Topics: HousingAnimal Type: Cephalopod, Invertebrate

Citation: Ponte, G., Roumbedakis, K., Galligioni, V. et al. 2023. General and species-specific recommendations for minimal requirements for the use of cephalopods in scientific research. Laboratory Animals 57(1), 26–39.

Non-surgical external jugular vein catheterization using an ear vein access in piglets

The objective of this study was to investigate the feasibility of external jugular vein catheterization through an ear vein in piglets. Forty-six sevoflurane-midazolam anaesthetized piglets were included. External jugular vein catheterization was conducted through the ear vein using the Seldinger...

Year Published: 2023Topics: Cannulation, Catheterization, & IntubationAnimal Type: Pig

Citation: Ringer, S. K., Schmid, A., Weiss, M. et al. 2023. Non-surgical external jugular vein catheterization using an ear vein access in piglets. Laboratory Animals 57(6), 642–649.

A minimally invasive bronchoscopic approach for direct delivery to murine airways and application to models of pulmonary infection

The laboratory mouse is used extensively for human disease modeling and preclinical therapeutic testing for efficacy, biodistribution, and toxicity. The variety of murine models available, and the ability to create new ones, eclipses all other species, but the size of...

Year Published: 2023Topics: Cannulation, Catheterization, & Intubation, Disease/Experimental ModelAnimal Type: Mouse, Rodent

Citation: Schelde, K., Rosenjack, J., Sonneborn, C. et al. 2023. A minimally invasive bronchoscopic approach for direct delivery to murine airways and application to models of pulmonary infection. Laboratory Animals 57(6), 611–622.

Evaluation of the effect of tramadol, paracetamol and metamizole on the severity of experimental colitis

Application of dextran sodium sulfate (DSS) is often used to induce experimental colitis. Current state of the art is to refrain from the use of analgesics due to their possible interaction with the model. However, the use of analgesics would...

Year Published: 2023Topics: Analgesia, Disease/Experimental ModelAnimal Type: Mouse, Rodent

Citation: Spalinger, M., Schwarzfischer, M., Niechcial, A. et al. 2023. Evaluation of the effect of tramadol, paracetamol and metamizole on the severity of experimental colitis. Laboratory Animals 57(5), 529–540.