Zebrafish

Clarification of early end-points for refinement of animal experiments, with specific reference to fish

Appropriate end-points are integral to the refinement of laboratory animal experiments. Our recent experience has highlighted that ambiguity around end-points is hampering their adoption in experiments that cause severe suffering to fish. In toxicology, the term endpoint (single word) refers...

Year Published: 2021Topics: Humane Endpoint, Welfare AssessmentAnimal Type: Fish, Other Fish, Salmon, Tilapia, Trout, Zebrafish

Citation: Ellis, T., Katsiadaki, I. 2021. Clarification of early end-points for refinement of animal experiments, with specific reference to fish. Laboratory Animals 55(3), 244-253.

Zebrafish water quality for laboratory animal technicians

Zebrafish are an increasingly popular model organism in many aspects of animal research; however, some aspects of their husbandry needs are quite disparate from that which most lab animal technicians are trained. Possibly the most important aspect of zebrafish husbandry...

Year Published: 2021Topics: Husbandry & ManagementAnimal Type: Fish, Zebrafish

Citation: Archer, C. 2021. Zebrafish water quality for laboratory animal technicians. Laboratory Animal Science Professional 9(4) (July/August), 11-13.

Plasticity of the skeleton and skeletal deformities in zebrafish (Danio rerio) linked to rearing density

The teleost zebrafish (Danio rerio), an established model for human skeletal diseases, is reared under controlled conditions with defined parameters for temperature and photoperiod. Studies aimed at defining the proper rearing density have been performed with regard to behavioural and...

Year Published: 2021Topics: Stocking DensityAnimal Type: Fish, Zebrafish

Citation: Martini, A., Huysseune, A., Witten, P. E. et al. 2021. Plasticity of the skeleton and skeletal deformities in zebrafish (Danio rerio) linked to rearing density. Journal of Fish Biology 98(4), 971-986.

Assessment of behaviour in groups of zebrafish (Danio rerio) using an intelligent software monitoring tool, the chromatic fish analyser

Background: Zebrafish (Danio rerio) are an increasingly popular model species within a variety of biomedical and neurobiological contexts. Researchers are required to prevent any negative states, such as pain, when using experimental animals to optimise fish welfare but analysis tools...

Year Published: 2019Topics: Welfare AssessmentAnimal Type: Fish, Zebrafish

Citation: Thomson, J. S., Al-Temeemy, A. A., Isted, H. et al. 2019. Assessment of behaviour in groups of zebrafish (Danio rerio) using an intelligent software monitoring tool, the chromatic fish analyser. Journal of Neuroscience Methods 328, 108433.

Enrichment for laboratory zebrafish—A review of the evidence and the challenges

Good practice for the housing and care of laboratory zebrafish Danio rerio is an increasingly discussed topic, with focus on appropriate water quality parameters, stocking densities, feeding regimes, anaesthesia and analgesia practices, methods of humane killing, and more. One area...

Year Published: 2021Topics: Environmental EnrichmentAnimal Type: Fish, Zebrafish

Citation: Stevens, C. H., Reed, B. T., Hawkins, P. 2021. Enrichment for laboratory zebrafish—A review of the evidence and the challenges. Animals 11(3), 698.

Identification of individual zebrafish (Danio rerio): A refined protocol for VIE tagging whilst considering animal welfare and the principles of the 3Rs

In aquatic ecology, studies have commonly employed a tagging technique known as visible implant elastomer (VIE). This method has not been widely adopted by the zebrafish research community and also lacks refinement with regard to animal welfare. The current paper...

Year Published: 2021Topics: Identification MethodAnimal Type: Fish, Zebrafish

Citation: Rácz, A., Allan, B., Dwyer, T. et al. 2021. Identification of individual zebrafish (Danio rerio): A refined protocol for VIE tagging whilst considering animal welfare and the principles of the 3Rs. Animals 11(3), 616.

Refining housing, husbandry and care for animals used in studies involving biotelemetry

Biotelemetry can contribute towards reducing animal numbers and suffering in disciplines including physiology, pharmacology and behavioural research. However, the technique can also cause harm to animals, making biotelemetry a ‘refinement that needs refining'. Current welfare issues relating to the housing...

Year Published: 2014Topics: External Bodily EquipmentAnimal Type: All/General, Amphibian, Baboon, Bird, Capuchin, Cat, Cattle, Cephalopod, Chicken, Chimpanzee, Chinchilla, Crocodile & Alligator, Crustacean, Dog, Equine, Ferret, Finch, Fish, Fowl, Frog & Toad, Gerbil, Gibbon, Goat, Guinea Pig, Hamster, Insect & Spider, Invertebrate, Lemur, Lizard, Macaque, Marine Mammal, Marmoset, Mink, Mole Rat, Mouse, Nonhuman Primate, Other Animal, Other Bird, Other Fish, Other Invertebrate, Other Nonhuman Primate, Other Rodent, Owl Monkey, Parrot, Pig, Rabbit, Rat, Reptile, Rodent, Salamander, Salmon, Shark & Ray, Sheep, Snake, Squirrel Monkey, Tilapia, Trout, Turtle & Tortoise, Vervet (African Green Monkey), Zebrafish

Citation: Hawkins, P. 2014. Refining housing, husbandry and care for animals used in studies involving biotelemetry. Animals 4(2), 361-373.

Behavioral Biology of Laboratory Animals (1st ed)

This 30-chapter volume informs students and professionals about the behavioral biology of animals commonly housed in laboratory and other captive settings. Each species evolved under specific environmental conditions, resulting in unique behavioral patterns, many of which are maintained in captivity...

Year Published: 2021Topics: Husbandry & Management, Natural Behavior, Welfare AssessmentAnimal Type: Amphibian, Baboon, Capuchin, Cat, Cattle, Chicken, Dog, Equine, Ferret, Fish, Fowl, Frog & Toad, Gerbil, Guinea Pig, Hamster, Lizard, Macaque, Marmoset, Mouse, Nonhuman Primate, Other Rodent, Owl Monkey, Pig, Rabbit, Rat, Reptile, Rodent, Salamander, Sheep, Snake, Squirrel Monkey, Turtle & Tortoise, Vervet (African Green Monkey), Zebrafish

Citation: Coleman, K., Shapiro, S. J. (Eds.). 2021. Behavioral Biology of Laboratory Animals (1st ed). CRC Press, Boca Raton, FL.

Animal-centric Care and Management – Enhancing Refinement in Biomedical Research

The concept of the 3Rs (Refinement, Reduction and Replacement) has been used as a framework for improving the welfare of laboratory animals for the last half century. By establishing an animal-centric view on housing and management, Animal-centric Care and Management:...

Year Published: 2021Topics: Abnormal/Problematic Behavior, Animal Training, Emotion, Pain, & Sentience, Environmental Enrichment, Handling, Housing, Human-Animal Interaction, Husbandry & Management, Natural Behavior, Social Housing & CompanionshipAnimal Type: All/General, Dog, Fish, Macaque, Marmoset, Mouse, Nonhuman Primate, Other Nonhuman Primate, Pig, Rabbit, Rat, Rodent, Zebrafish

Citation: Sørensen, D., Cloutier, S., Gaskill, B. (Eds.). 2021. Animal-centric Care and Management - Enhancing Refinement in Biomedical Research. CRC Press, Boca Raton, FL.

Laterality and fish welfare – A review

Humans interact with fishes in many contexts including aquaculture, scientific study and companion animals. In all of these contexts, fish welfare can be compromised through anthropogenic means. Concern for fish welfare has grown considerably in recent years, with many states...

Year Published: 2021Topics: Welfare AssessmentAnimal Type: Fish, Other Fish, Salmon, Tilapia, Trout, Zebrafish

Citation: Berlinghieri, F., Panizzon, P., Penry-Williams, I. L. et al. 2021. Laterality and fish welfare - A review. Applied Animal Behaviour Science 236, 105239.