Amphibian

Photo-based individual identification is more reliable than visible implant elastomer tags or toe-tipping in young agile frogs

In amphibian capture-recapture studies, commonly used individual-identification methods include toe clipping as well as less invasive alternatives of varying cost. Yet, choosing the best method for a study is challenging, because both the reliability for identification and the severity of...

Year Published: 2026Topics: Identification MethodAnimal Type: Amphibian, Frog & Toad

Citation: Nemesházi, E., Ujhegyi, N., Mikó, Z. et al. 2026. Photo-based individual identification is more reliable than visible implant elastomer tags or toe-tipping in young agile frogs. PLOS ONE 21(2), e0342340.

Identification and validation of operational pain indicators in anurans

Amphibian welfare, particularly pain assessment in anurans, remains understudied despite their ecological and biomedical significance. This study aimed to identify and validate operational pain indicators for adult anurans under professional care. A four-phase approach was used: a systematic literature review,...

Year Published: 2025Topics: Emotion, Pain, & Sentience, Welfare AssessmentAnimal Type: Amphibian, Frog & Toad

Citation: González, S., Caiozzi, A., Cabeza, O. et al. 2025. Identification and Validation of Operational Pain Indicators in Anurans. Journal of Zoological and Botanical Gardens 6(4).

Towards more compassionate wildlife research through the 3Rs principles: Moving from invasive to noninvasive methods

Research in ecology and wildlife biology remains crucial for increasing our knowledge and improving species management and conservation in the midst of the current biodiversity crisis. However, obtaining information on population status often involves invasive sampling of a certain number...

Year Published: 2020Topics: Biological Sampling & Physiological Measurement, Identification Method, RestraintAnimal Type: All/General, Amphibian, Crocodile & Alligator, Fish, Frog & Toad, Lizard, Nonhuman Primate, Other Animal, Other Fish, Reptile, Salamander, Snake, Turtle & Tortoise

Citation: Zemanova, M. A. 2020. Towards more compassionate wildlife research through the 3Rs principles: Moving from invasive to noninvasive methods. Wildlife Biology 2020(1), no page numbers.

The effect of shelter on oxidative stress and aggressive behavior in crested newt larvae (Triturus spp.)

Shelters are important for animal survival. Provision of adequate hiding places allow animals to express their natural sheltering behavior and it can have different positive effects on cortisol levels, physiological processes and mental performance. Although the absence of a refuge...

Year Published: 2020Topics: Environmental Enrichment, HousingAnimal Type: Amphibian, Salamander

Citation: Petrović, T. G., Vučić, T. Z., Nikolić, S. Z. et al. 2020. The effect of shelter on oxidative stress and aggressive behavior in crested newt larvae (Triturus spp.). Animals 10(4), 603.

Behavioral diversity as a potential indicator of positive animal welfare

Modern day zoos and aquariums continuously assess the welfare of their animals and use evidence to make informed management decisions. Historically, many of the indicators of animal welfare used to assess the collection are negative indicators of welfare, such as...

Year Published: 2020Topics: Natural Behavior, Welfare AssessmentAnimal Type: All/General, Amphibian, Nonhuman Primate, Reptile

Citation: Miller, L. J., Vicino, G. A., Sheftel, J. et al. 2020. Behavioral diversity as a potential indicator of positive animal welfare. Animals 10(7), 1211.

Evaluation of effective and practical euthanasia methods for larval African clawed frogs (Xenopus laevis)

Larval, or tadpole-stage Xenopus laevis frogs are a popular research model for developmental biology and disease studies. Existing euthanasia guidance documents offer recommendations for both eggs and adult stages, yet do not specifically address the larval stage. Data evaluating effective...

Year Published: 2020Topics: EuthanasiaAnimal Type: Amphibian, Frog & Toad

Citation: Galex, I. A., Gallant, C. M., D'Avignon, N. et al. 2020. Evaluation of effective and practical euthanasia methods for larval African clawed frogs (Xenopus laevis). JAALAS 59(3), 269–274.

Quantitative and qualitative behavioral measurements to assess pain in axolotls (Ambystoma mexicanum)

Effective pain relief in animals relies on the ability to discern pain and assess its severity. However, few objective measures exist to assess the presence and severity of pain in axolotls, and few resources are available regarding drugs and appropriate...

Year Published: 2020Topics: Welfare AssessmentAnimal Type: Amphibian, Salamander

Citation: Llaniguez, J. T., Szczepaniak, M. A., Rickman, B. H. et al. 2020. Quantitative and qualitative behavioral measurements to assess pain in axolotls (Ambystoma mexicanum). JAALAS 59(2), 186–196.

Behavioral Biology of Laboratory Animals

This 30-chapter volume informs students and professionals about the behavioral biology of animals commonly housed in laboratory and other captive settings. Each species evolved under specific environmental conditions, resulting in unique behavioral patterns, many of which are maintained in captivity...

Year Published: 2022Topics: Abnormal/Problematic Behavior, Animal Training, Environmental Enrichment, Handling, Housing, Human-Animal Interaction, Husbandry & Management, Natural Behavior, Rearing & Weaning, Social Housing & Companionship, Welfare AssessmentAnimal Type: All/General, Amphibian, Baboon, Capuchin, Cat, Cattle, Chicken, Crocodile & Alligator, Dog, Equine, Ferret, Finch, Fish, Fowl, Frog & Toad, Gerbil, Guinea Pig, Hamster, Lizard, Macaque, Marmoset, Mouse, Nonhuman Primate, Other Rodent, Owl Monkey, Pig, Rabbit, Rat, Reptile, Rodent, Salamander, Sheep, Snake, Squirrel Monkey, Turtle & Tortoise, Vervet (African Green Monkey), Zebrafish

Citation: Coleman, K., Schapiro, S. J. (Eds.) 2022. Behavioral Biology of Laboratory Animals (1st Ed.). CRC Press, Boca Raton, FL, 560 p.

Standard methods for marking caudate amphibians do not impair animal welfare over the short term: An experimental approach

Major advancements in ecology and biodiversity conservation have been made thanks to methods for marking and individually tracking animals. Marking animals is both widely used and controversial due to the potential consequences for animal welfare, which are often incompletely evaluated...

Year Published: 2024Topics: Handling, Identification MethodAnimal Type: Amphibian, Salamander

Citation: Daversa, D. R., Baxter, E., Rosa, G. M. et al. 2024. Standard methods for marking caudate amphibians do not impair animal welfare over the short term: An experimental approach. Animal Welfare 33, e24.

Effectiveness, safety, and pharmacokinetics of meloxicam formulations in African-clawed frogs, Xenopus laevis

Pain management in amphibians is an emerging field of veterinary medicine with only a limited number of analgesics studied for their efficacy. The African-clawed frog, Xenopus laevis, is a popular animal model in research due to its oocyte morphology and...

Year Published: 2024Topics: AnalgesiaAnimal Type: Amphibian, Frog & Toad

Citation: Leung, G., Diaz, L. L., Monette, S. et al. 2024. Effectiveness, safety, and pharmacokinetics of meloxicam formulations in African-clawed frogs, Xenopus laevis. Comparative Medicine 74(5), 336–343.