Mouse

Affective state determination in a mouse model of colitis-associated colorectal cancer

Behavioural indicators of affective state, including burrowing, clinical scores and the Mouse Grimace Score have not yet been validated in mouse models of chronic gastrointestinal disease. Additionally, a comparison of these methods has not been characterised. This study aimed to...

Year Published: 2020Topics: Disease/Experimental Model, Welfare AssessmentAnimal Type: Mouse, Rodent

Citation: Chartier, L. C., Hebart, M. L., Howarth, G. S. et al. 2020. Affective state determination in a mouse model of colitis-associated colorectal cancer. PLOS ONE 15(1), e0228413.

Alternative anesthesia of neonatal mice for global rAAV delivery in the brain with non-detectable behavioral interference in adults

Viral-transduced gene expression is the current standard for cell-type-specific labeling and cell tacking in experimental neuroscience. To achieve widespread gene expression, a viral delivery method to neonatal rodents was introduced more than two decades ago. Most of those neonatal viral...

Year Published: 2020Topics: Anesthesia & Sedation, Disease/Experimental ModelAnimal Type: Mouse, Rodent

Citation: Tang, W., Zillmann, U., Sprengel, R. 2020. Alternative anesthesia of neonatal mice for global rAAV delivery in the brain with non-detectable behavioral interference in adults. Frontiers in Behavioral Neuroscience 14, 115.

Alternatives to shipping live mice

Many in the lab animal field are familiar with shipping animals from vendor to investigator, from investigator to vendor, and from investigator to investigator. Sharing mouse models represents being good stewards of our animal resources and prevents unnecessary duplication of...

Year Published: 2020Topics: Relocation & Transport, ReproductionAnimal Type: Mouse, Rodent

Citation: Larson, M. A. 2020. Alternatives to shipping live mice. Laboratory Animal Science Professional 8(5) (September/October), 52-54.

Automated radial 8-arm maze: A voluntary and stress-free behavior test to assess spatial learning and memory in mice

The radial arm maze (RAM) is a common behavioral test to assess spatial working and reference memory in mice. However, conventional RAM experiments require a substantial degree of manual handling and animals are usually subjected to prolonged periods of food...

Year Published: 2020Topics: Behavioral & Cognitive TestingAnimal Type: Mouse, Rodent

Citation: Mei, J., Kohler, J., Winter, Y. et al. 2020. Automated radial 8-arm maze: A voluntary and stress-free behavior test to assess spatial learning and memory in mice. Behavioural Brain Research 381, 112352.

Benefits of tunnel handling persist after repeated restraint, injection and anaesthesia

Millions of mice are used every year for scientific research, representing the majority of scientific procedures conducted on animals. The standard method used to pick up laboratory mice for general husbandry and experimental procedures is known as tail handling and...

Year Published: 2020Topics: HandlingAnimal Type: Mouse, Rodent

Citation: Henderson, L. J., Dani, B., Serrano, E. M. N., et al. 2020. Benefits of tunnel handling persist after repeated restraint, injection and anaesthesia. Scientific Reports 10(1), 14562.

Buccal swab based genotyping of genetically modified mice

Routine sample collection for genotyping genetically modified animals (rodents) (GMAs) typically includes tail snip, toe clip, or ear biopsy. To decrease the potential pain and distress caused by tissue sample collection for genetic analysis, we desired to use a nonsurgical...

Year Published: 2020Topics: Identification MethodAnimal Type: Mouse, Rodent

Citation: Huang, J., Kayne, P. S. 2020. Buccal swab based genotyping of genetically modified mice. Laboratory Animal Science Professional 8(5) (September/October), 64-66.

Clinical management of pain in rodents

The use of effective regimens for mitigating pain remain underutilized in research rodents despite the general acceptance of both the ethical imperative and regulatory requirements intended to maximize animal welfare. Factors contributing to this gap between the need for and...

Year Published: 2020Topics: AnalgesiaAnimal Type: Guinea Pig, Mouse, Rat, Rodent

Citation: Foley, P. L., Kendall, L. V., Turner, P. V. 2019. Clinical management of pain in rodents. Comparative Medicine 69(6), 468–489.

Comparing distress of mouse models for liver damage

In order to foster animal welfare as well as high quality of research, many countries regulate by law that the severity of animal experiments must be evaluated and considered when performing biomedical research. It is well accepted that multiple parameters...

Year Published: 2020Topics: Disease/Experimental Model, Welfare AssessmentAnimal Type: Mouse, Rodent

Citation: Tang, G., Seume, N., Häger, C. et al. 2020. Comparing distress of mouse models for liver damage. Scientific Reports 10(1), 19814.

Continuous and non-invasive thermography of mouse skin accurately describes core body temperature patterns, but not absolute core temperature

Body temperature is an important physiological parameter in many studies of laboratory mice. Continuous assessment of body temperature has traditionally required surgical implantation of a telemeter, but this invasive procedure adversely impacts animal welfare. Near-infrared thermography provides a non-invasive alternative...

Year Published: 2020Topics: Biological Sampling & Physiological MeasurementAnimal Type: Mouse, Rodent

Citation: van der Vinne, V., Pothecary, C. A., Wilcox, S. L. et al. 2020. Continuous and non-invasive thermography of mouse skin accurately describes core body temperature patterns, but not absolute core temperature. Scientific Reports 10(1), 20680.

Defining body-weight reduction as a humane endpoint: A critical appraisal

In many animal experiments scientists and local authorities define a body-weight reduction of 20% or more as severe suffering and thereby as a potential parameter for humane endpoint decisions. In this study, we evaluated distinct animal experiments in multiple research...

Year Published: 2020Topics: Disease/Experimental Model, Humane Endpoint, Welfare AssessmentAnimal Type: Mouse, Rat, Rodent

Citation: Talbot, S. R., Biernot, S., Bleich, A. et al. 2020. Defining body-weight reduction as a humane endpoint: A critical appraisal. Laboratory Animals 54(1), 99–110.