Mouse

Impact of three commonly used blood sampling techniques on the welfare of laboratory mice: Taking the animal’s perspective

Laboratory mice are the most frequently used animals in biomedical research. In accordance with guidelines for humane handling, several blood sampling techniques have been established. While the effects of these procedures on blood quality and histological alterations at the sampling...

Year Published: 2020Topics: Biological Sampling & Physiological MeasurementAnimal Type: Mouse, Rodent

Citation: Meyer, N., Kröger, M., Thümmler, J. et al. 2020. Impact of three commonly used blood sampling techniques on the welfare of laboratory mice: Taking the animal’s perspective. PLOS ONE 15(9), e0238895.

Improvement of the Mouse Grimace Scale set-up for implementing a semi-automated Mouse Grimace Scale scoring (Part 1)

The Mouse Grimace Scale (MGS) has been widely used for the noninvasive examination of distress/pain in mice. The aim of this study was to further improve its performance to generate repeatable, faster, blinded and reliable results for developing automated and...

Year Published: 2020Topics: Welfare AssessmentAnimal Type: Mouse, Rodent

Citation: Ernst, L., Kopaczka, M., Schulz, M. et al. 2020. Improvement of the Mouse Grimace Scale set-up for implementing a semi-automated Mouse Grimace Scale scoring (Part 1). Laboratory Animals 54(1), 83–91.

Impulse for animal welfare outside the experiment

Animal welfare is a growing societal concern and the well-being of animals used for experimental purposes is under particular scrutiny. The vast majority of laboratory animals are mice living in small cages that do not offer very much variety. Moreover,...

Year Published: 2020Topics: HousingAnimal Type: Mouse, Rodent

Citation: Lewejohann, L., Schwabe, K., Häger, C. et al. 2020. Impulse for animal welfare outside the experiment. Laboratory Animals 54(2), 150–158.

It’s Okay to Cry – Discussions by the Laboratory Animal Refinement & Enrichment Forum [LAREF], Volume V

This is the 5th volume of selected discussions that took place on the electronic Laboratory Animal Refinement & Enrichment Forum between February 2016 and December 2019. The forum was created in October 2002; it allows the international animal care community...

Year Published: 2020Topics: Abnormal/Problematic Behavior, Animal Training, Biological Sampling & Physiological Measurement, Drug/Substance Administration, Environmental Enrichment, External Bodily Equipment, Housing, Human-Animal Interaction, Relocation & TransportAnimal Type: Cat, Chinchilla, Dog, Ferret, Guinea Pig, Macaque, Marmoset, Mouse, Nonhuman Primate, Pig, Rabbit, Rat, Rodent

Citation: Reinhardt, V. (ed.) 2020. It’s Okay to Cry - Discussions by the Laboratory Animal Refinement & Enrichment Forum [LAREF], Volume V. Animal Welfare Institute, Washington, DC.

Long-term effect of environmental enrichment on reproductive performance of Swiss Webster mice and their female offspring

The aim of this study was to evaluate the effect of an environmental enrichment (EE) plan on the reproductive performance of Swiss Webster mice and their female offspring used as recipients for embryo transfer. A total of 54 breeder mice...

Year Published: 2020Topics: Environmental EnrichmentAnimal Type: Mouse, Rodent

Citation: Meikle, M. N., Arévalo, A. P., Schlapp, G. et al. 2020. Long-term effect of environmental enrichment on reproductive performance of Swiss Webster mice and their female offspring. Animals 10(8), 1438.

Oestrus checking – increasing productivity and embracing the 3R’s

Much like the menstrual cycle in humans, mice also have a cycle where the eggs ripen, ovulation takes place, the females come into heat and are receptive to mate with the male; this occurs over the space of 4-5 days...

Year Published: 2020Topics: ReproductionAnimal Type: Mouse, Rodent

Citation: Hoskins, S., Brown, J. 2020. Oestrus checking – increasing productivity and embracing the 3R’s. Animal Technology and Welfare 19(2), 159-161.

Optimising streptozotocin dosing to minimise renal toxicity and impairment of stomach emptying in male 129/Sv mice

The streptozotocin (STZ)-induced diabetic mouse model has been extensively used as a model for diabetes and diabetic nephropathy, but it is still influenced by many off-target toxic effects and large variation in diabetes induction. Therefore, the aim of this study...

Year Published: 2020Topics: Disease/Experimental ModelAnimal Type: Mouse, Rodent

Citation: Nørgaard, S. A., Søndergaard, H., Sørensen, D. B. et al. 2020. Optimising streptozotocin dosing to minimise renal toxicity and impairment of stomach emptying in male 129/Sv mice. Laboratory Animals 54(4), 341–352.

Performance of severity parameters to detect chemotherapy-induced pain and distress in mice

According to European Union directive 2010/63/EU a severity classification of experimental procedures performed on laboratory animals is mandatory. This includes a prospective evaluation of all interventions performed within the experiment, as well as an assessment of the actual burden of...

Year Published: 2020Topics: Disease/Experimental Model, Welfare AssessmentAnimal Type: Mouse, Rodent

Citation: Rix, A., Drude, N., Mrugalla, A. et al. 2020. Performance of severity parameters to detect chemotherapy-induced pain and distress in mice. Laboratory Animals 54(5), 452-460.

Progressing the care, husbandry and management of ageing mice used in scientific studies

Driven by the longer lifespans of humans, particularly in Westernised societies, and the need to know more about ‘healthy ageing’, ageing mice are being used increasingly in scientific research. Many departments and institutes involved with ageing research have developed their...

Year Published: 2020Topics: Disease/Experimental Model, Humane Endpoint, Welfare AssessmentAnimal Type: Mouse, Rodent

Citation: Wilkinson, M. J. A., Selman, C., McLaughlin, L. et al. 2020. Progressing the care, husbandry and management of ageing mice used in scientific studies. Laboratory Animals 54(3) 225–238.

Manganese-free chow, a refined non-invasive solution to reduce gastrointestinal signal for T1-weighted magnetic resonance imaging of the mouse abdomen

Commercial mouse chow is designed to provide a complete, nutrient-rich diet, and it can contain upwards of 100 mg/kg manganese, an essential mineral. Manganese acts as a relaxation time-shortening contrast agent for both T1 and T2, and where standard chow is...

Year Published: 2020Topics: Husbandry & ManagementAnimal Type: Mouse, Rodent

Citation: Kersemans, V., Wallington, S., Allen, P. D. et al. 2020. Manganese-free chow, a refined non-invasive solution to reduce gastrointestinal signal for T1-weighted magnetic resonance imaging of the mouse abdomen. Laboratory Animals 54(4), 353–364.