Mouse

Intraperitoneal administration of ethanol as a means of euthanasia for neonatal mice (Mus musculus)

The humane euthanasia of animals in research is of paramount importance. Neonatal mice frequently respond differently to euthanasia agents when compared with adults. The AVMA's Guidelines for the Euthanasia of Animals includes intraperitoneal injection of ethanol as "acceptable with conditions,"...

Year Published: 2017Topics: EuthanasiaAnimal Type: Mouse, Rodent

Citation: de Souza Dyer, C., Brice, A. K., Marx, J. O. 2017. Intraperitoneal administration of ethanol as a means of euthanasia for neonatal mice (Mus musculus). JAALAS 56(3), 299-306.

Comparing phlebotomy by tail tip amputation, facial vein puncture, and tail vein incision in C57BL/6 mice by using physiologic and behavioral metrics of pain and distress

Tail tip amputation with minimal restraint is not widely used for mouse phlebotomy. In part, this infrequency may reflect policies influenced by tail tip amputation procedures for genotyping, which involve greater handling and tissue removal. To assess tail tip amputation...

Year Published: 2017Topics: Biological Sampling & Physiological MeasurementAnimal Type: Mouse, Rodent

Citation: Moore, E. S., Cleland, T. A., Williams, W. O. et al. 2017. Comparing phlebotomy by tail tip amputation, facial vein puncture, and tail vein incision in C57BL/6 mice by using physiologic and behavioral metrics of pain and distress. JAALAS 56(3), 307-317.

Effect of home cage bedding in the induction chamber on serum cortisol and corticosterone levels in response to isoflurane-induced anesthesia in C57BL/6J mice

Mice are routinely anesthetized with isoflurane in an induction chamber. The AVMA Guidelines for the Euthanasia of Animals states that distress should be minimized during euthanasia but does not address this point in regard to induction of anesthesia. Here we...

Year Published: 2017Topics: Anesthesia & SedationAnimal Type: Mouse, Rodent

Citation: Reiter, C. P., Christy, A. C., Olsen, C. H. et al. 2017. Effect of home cage bedding in the induction chamber on serum cortisol and corticosterone levels in response to isoflurane-induced anesthesia in C57BL/6J mice. JAALAS 56(2), 118-121.

An efficient, simple, and noninvasive procedure for genotyping aquatic and nonaquatic laboratory animals

Various animal models are indispensible in biomedical research. Increasing awareness and regulations have prompted the adaptation of more humane approaches in the use of laboratory animals. With the development of easier and faster methodologies to generate genetically altered animals, convenient...

Year Published: 2017Topics: Identification MethodAnimal Type: Amphibian, Frog & Toad, Mouse, Rodent

Citation: Okada, M., Miller, T. C., Roediger, J. et al. 2017. An efficient, simple, and noninvasive procedure for genotyping aquatic and nonaquatic laboratory animals. JAALAS 56(5), 570-573.

Effects of intracage ammonia on markers of pulmonary endothelial integrity in mice housed in static microisolation cages

Time-weighted exposure limits to ammonia are established for humans; however similar guidelines have not been defined for laboratory rodents. The Guide recommends maintaining air pollutants at concentrations below levels irritating to mucous membranes but does not provide specific values. Numerous...

Year Published: 2018Topics: Husbandry & ManagementAnimal Type: Mouse, Rodent

Citation: Eichner, M., Purcell, J. E., Fortman, J. D. 2018. Effects of intracage ammonia on markers of pulmonary endothelial integrity in mice housed in static microisolation cages. JAALAS 57(1), 18-23.

Evaluation of a commercially available euthanasia solution as a voluntarily ingested euthanasia agent in laboratory mice

All currently accepted methods of euthanasia for laboratory mice involve some degree of stress, fear, anxiety, or pain. We evaluated the voluntary oral administration of a euthanasia drug in 99 male and 81 female mice of various strains. We first...

Year Published: 2018Topics: EuthanasiaAnimal Type: Mouse, Rodent

Citation: Dudley, E. S., Boivin, G. P. 2018. Evaluation of a commercially available euthanasia solution as a voluntarily ingested euthanasia agent in laboratory mice. JAALAS 57(1), 30-34.

PCR testing of IVC filter tops as a method for detecting murine pinworms and fur mites

We evaluated PCR testing of filter tops from cages maintained on an IVC system through which exhaust air is filtered at the cage level as a method for detecting parasite-infected and -infested cages. Cages containing 4 naïve Swiss Webster mice...

Year Published: 2017Topics: Husbandry & ManagementAnimal Type: Mouse, Rodent

Citation: Gerwin, P. M., Ricart Arbona, R. J., Riedel, E. R. et al. 2017. PCR testing of IVC filter tops as a method for detecting murine pinworms and fur mites. JAALAS 56(6), 752-761.

Effects of repeated intraperitoneal injection of pharmaceutical-grade and nonpharmaceutical-grade corn oil in female C57BL/6J mice

Due to potential adverse effects on animal wellbeing, the use of nonpharmaceutical-grade substances in animal research must be scientifically justified in cases where a pharmaceutical-grade version of the substance exists. This requirement applies to all substances, including vehicles used to...

Year Published: 2017Topics: Drug/Substance AdministrationAnimal Type: Mouse, Rodent

Citation: Hubbard, J. S., Chen, P. H., Boyd, K. L. 2017. Effects of repeated intraperitoneal injection of pharmaceutical-grade and nonpharmaceutical-grade corn oil in female C57BL/6J mice. JAALAS 56(6), 779-785.

Review of CO2 as a euthanasia agent for laboratory rats and mice

Selecting an appropriate, effective euthanasia agent is controversial. Several recent publications provide clarity on the use of CO2 in laboratory rats and mice. This review examines previous studies on CO2 euthanasia and presents the current body of knowledge on the...

Year Published: 2017Topics: EuthanasiaAnimal Type: Mouse, Rat, Rodent

Citation: Boivin, G. P., Hickman, D. L., Creamer-Hente, M. A. et al. 2017. Review of CO2 as a euthanasia agent for laboratory rats and mice. JAALAS 56(5), 491-499.

Quantification of induced hypothermia from aseptic scrub applications during rodent surgery preparation

Laboratory mice (Mus musculus) are prone to develop hypothermia during anesthesia for surgery, thus potentially impeding anesthetic recovery, wound healing, and future health. The core body temperatures of isoflurane-anesthetized mice are influenced by the choice of supplemental heat sources; however,...

Year Published: 2017Topics: Anesthesia & SedationAnimal Type: Mouse, Rodent

Citation: Skorupski, A. M., Zhang, J., Ferguson, D. et al. 2017. Quantification of induced hypothermia from aseptic scrub applications during rodent surgery preparation. JAALAS 56(5), 562-569.