Mouse

Evaluation of active warming and surgical draping for perioperative thermal support in laboratory mice

Surgical procedures are commonly performed using mice but can have major effects on their core body temperature, including development of hypothermia. In this study, we evaluated active perioperative warming with and without surgical draping with adherent plastic wrap to refine...

Year Published: 2022Topics: Surgery & Post-OpAnimal Type: Mouse, Rodent

Citation: Bailey, K. T., Jantre, S. R., Lawrence, F. R. et al. 2022. Evaluation of active warming and surgical draping for perioperative thermal support in laboratory mice. JAALAS 61(5), 482-494.

Refinements to rodent head fixation and fluid/food control for neuroscience

The use of head fixation in mice is increasingly common in research, its use having initially been restricted to the field of sensory neuroscience. Head restraint has often been combined with fluid control, rather than food restriction, to motivate behaviour,...

Year Published: 2022Topics: Behavioral & Cognitive Testing, RestraintAnimal Type: Mouse, Rodent

Citation: Barkus, C., Bergmann, C., Branco, T. et al. 2022. Refinements to rodent head fixation and fluid/food control for neuroscience. Journal of Neuroscience Methods 381, 109705.

3R measures in facilities for the production of genetically modified rodents

Sociocultural changes in the human–animal relationship have led to increasing demands for animal welfare in biomedical research. The 3R concept is the basis for bringing this demand into practice: Replace animal experiments with alternatives where possible, Reduce the number of...

Year Published: 2022Topics: Analgesia, Disease/Experimental Model, Husbandry & Management, Reproduction, Surgery & Post-OpAnimal Type: Mouse, Rat, Rodent

Citation: Zevnik, B., Jerchow, B., Buch, T. 2022. 3R measures in facilities for the production of genetically modified rodents. Lab Animal 51(6), 162-177.

A quick and low-intensity method for oral administration to large numbers of mice: A possible alternative to oral gavage

Oral administration of medication to experimental animals is a cause of significant stress. When coupled to animals who are already under strenuous circumstances due to the disease being modelled, there is a significant risk for increased morbidity and mortality, thus...

Year Published: 2022Topics: Drug/Substance AdministrationAnimal Type: Mouse, Rodent

Citation: Neto, T., Faustino-Rocha, A. I., Gil da Costa, R. M. et al. 2022. A quick and low-intensity method for oral administration to large numbers of mice: A possible alternative to oral gavage. Laboratory Animals 56(2), 185-190.

Early-life mice housed in standard stocking density reduce the spontaneous physical activity and increase visceral fat deposition before reaching adulthood

Laboratory rodents spend the entire day housed in standard cages that provide a restricted area for movements and might, therefore, limit physical activity. However, it has not been tested in immature rodents of ages ranging from weaning to adulthood (adolescence...

Year Published: 2022Topics: Stocking DensityAnimal Type: Mouse, Rodent

Citation: Scariot, P. P., Gobatto, C. A., Polisel, E. E. et al. 2022. Early-life mice housed in standard stocking density reduce the spontaneous physical activity and increase visceral fat deposition before reaching adulthood. Laboratory Animals 56(4), 344-355.

Minimal exposure times for irreversible euthanasia with carbon dioxide in mice and rats

When using an anesthetic overdose to euthanize laboratory rodents, a secondary method of euthanasia is recommended to ensure that the apparent death is irreversible. This secondary method usually is accomplished through the collection of tissues that are required to complete...

Year Published: 2022Topics: EuthanasiaAnimal Type: Mouse, Rat, Rodent

Citation: Hickman, D. L. 2022. Minimal exposure times for irreversible euthanasia with carbon dioxide in mice and rats. JAALAS 61(3), 283-286.

A comparison of blood collection techniques in mice and their effects on welfare

Multiple methods are used to collect blood from mice; these methods have different effects on animal welfare. This study compared blood collection from facial, chin, and saphenous locations with regard to various parameters, including the time needed to collect blood,...

Year Published: 2022Topics: Biological Sampling & Physiological MeasurementAnimal Type: Mouse, Rodent

Citation: Ahrens Kress, A. P., Zhang, Y., Kaiser-Vry, A. R. et al. 2022. A Comparison of blood collection techniques in mice and their effects on welfare. JAALAS 61(3), 287-295.

Physiologic and behavioral effects in mice anesthetized with isoflurane in a red-tinted or a traditional translucent chamber

Isoflurane has been characterized as a distressing agent for rodents, causing both physiologic and behavioral effects. Using a "darkened home cage" has been recommended during CO2 administration for rodent euthanasia; this is arguably a similar animal experience to anesthetic induction...

Year Published: 2022Topics: Anesthesia & Sedation, EuthanasiaAnimal Type: Mouse, Rodent

Citation: McKinney, M. M., Dupont, W. D., Corson, K. J. et al. 2022. Physiologic and behavioral effects in mice anesthetized with isoflurane in a red-tinted or a traditional translucent chamber. JAALAS 61(4), 322-332.

Using sterile flocked swabs as an alternative method for rodent health monitoring

Routine health monitoring is an integral part of managing SPF rodent colonies. In recent years, rack-level environmental sampling has been introduced as an adjunct method or replacement for exposure of sentinel rodents to soiled bedding. However, rack-level environmental monitoring is...

Year Published: 2022Topics: Husbandry & ManagementAnimal Type: Mouse, Rat, Rodent

Citation: Varela, M. M. D., Bibay, J. I. A., Ogden, B. E. et al. 2022. Using sterile flocked swabs as an alternative method for rodent health monitoring. JAALAS 61(4), 370-380.

Assessment of the safety and efficacy of pre-emptive use of extended-release buprenorphine for mouse laparotomy

Buprenorphine is commonly used to control postoperative pain in rodents. Short-acting formulations of buprenorphine (bup-HCl) require frequent handling and restraint of animals for appropriate dosing, which can be stressful and confound research outcomes. Ethiqa XR (bup-ER) is an FDA-indexed extended-release...

Year Published: 2022Topics: AnalgesiaAnimal Type: Mouse, Rodent

Citation: Chan, G., Si, C., Nichols, M. R. et al. 2022. Assessment of the safety and efficacy of pre-emptive use of extended-release buprenorphine for mouse laparotomy. JAALAS 61(4), 381-387.