Mouse

Mouse isoflurane anesthesia using the drop method

Anesthesia with isoflurane prior to carbon dioxide euthanasia is recommended as a refinement, but vaporizer access can be limited. An alternative to vaporizers is the ‘drop' method, introducing a fixed volume of isoflurane into the induction chamber. Previous work suggests...

Year Published: 2023Topics: Anesthesia & Sedation, EuthanasiaAnimal Type: Mouse, Rodent

Citation: Bodnar, M. J., Ratuski, A. S., Weary, D. M. 2023. Mouse isoflurane anesthesia using the drop method. Laboratory Animals 57(6), 623–630.

Determining the preferred liquid reward in adult C57BL/6 mice

Using food rewards to motivate mice is commonplace in behavioural research. Using a reward which is highly desirable is of benefit, as it can reduce the need to food restrict animals and can encourage higher levels of consumption, allowing for...

Year Published: 2023Topics: Behavioral & Cognitive Testing, Drug/Substance AdministrationAnimal Type: Mouse, Rodent

Citation: Miller, A. L., Leach, M. C. 2023. Determining the preferred liquid reward in adult C57BL/6 mice. Laboratory Animals 57(3), 332–335.

A minimally invasive bronchoscopic approach for direct delivery to murine airways and application to models of pulmonary infection

The laboratory mouse is used extensively for human disease modeling and preclinical therapeutic testing for efficacy, biodistribution, and toxicity. The variety of murine models available, and the ability to create new ones, eclipses all other species, but the size of...

Year Published: 2023Topics: Cannulation, Catheterization, & Intubation, Disease/Experimental ModelAnimal Type: Mouse, Rodent

Citation: Schelde, K., Rosenjack, J., Sonneborn, C. et al. 2023. A minimally invasive bronchoscopic approach for direct delivery to murine airways and application to models of pulmonary infection. Laboratory Animals 57(6), 611–622.

Evaluation of the effect of tramadol, paracetamol and metamizole on the severity of experimental colitis

Application of dextran sodium sulfate (DSS) is often used to induce experimental colitis. Current state of the art is to refrain from the use of analgesics due to their possible interaction with the model. However, the use of analgesics would...

Year Published: 2023Topics: Analgesia, Disease/Experimental ModelAnimal Type: Mouse, Rodent

Citation: Spalinger, M., Schwarzfischer, M., Niechcial, A. et al. 2023. Evaluation of the effect of tramadol, paracetamol and metamizole on the severity of experimental colitis. Laboratory Animals 57(5), 529–540.

Effects of depilatory cream formulation and contact time on mouse skin

Depilatory creams are widely used in research to remove hair in preparation for surgery, imaging, and other procedures. However, few studies have evaluated the effects of these creams on mouse skin. We sought to determine the cutaneous effects of 2...

Year Published: 2023Topics: Surgery & Post-OpAnimal Type: Mouse, Rodent

Citation: Reichert, M. N., Koewler, N. J., Hargis, A. M. et al. 2023. Effects of depilatory cream formulation and contact time on mouse skin. JAALAS 62(2), 153–162.

Use of a low-calorie flavored gel to facilitate oral self-administration of analgesics in mice

The goals of this study were to determine whether mice would adapt to a low-calorie flavored water gel as their sole source of hydration and whether the addition of acetaminophen, tramadol, meloxicam, or buprenorphine to the gel would affect their...

Year Published: 2023Topics: Analgesia, Drug/Substance AdministrationAnimal Type: Mouse, Rodent

Citation: Riddell, D. L., Hyndman, T. H., Bowden, R. S. et al. 2023. Use of a low-calorie flavored gel to facilitate oral self-administration of analgesics in mice. JAALAS 62(2), 163–169.

Effects of different grades of carbon dioxide on euthanasia of mice (Mus musculus)

Carbon dioxide (CO2) is a conditionally acceptable method for mouse euthanasia, according to the American Veterinary Medical Association Guidelines for the Euthanasia of Animals. In a 2012 Office of Laboratory Animal Welfare (OLAW) webinar, representatives of OLAW, the USDA and...

Year Published: 2023Topics: EuthanasiaAnimal Type: Mouse, Rodent

Citation: Stuckey, J. E., Makhija, S. D., Reimer, D. C. et al. 2023. Effects of different grades of carbon dioxide on euthanasia of mice (Mus musculus). JAALAS 62(5), 430–437.

Enriched environment during adolescence modulates lipid metabolism and emotion-related behaviors in mice

Enriched environment (EE) is an important animal experimental paradigm to decipher gene-environment interaction. It is thought to be efficient in aiding recovery from certain metabolism disorders or cognitive impairments. Recently, the effects of EE during adolescence in mice gradually draw...

Year Published: 2023Topics: Environmental EnrichmentAnimal Type: Mouse, Rodent

Citation: Xu, L., Jiao, M., Cui, Z.-L. et al. 2023. Enriched environment during adolescence modulates lipid metabolism and emotion-related behaviors in mice. Journal of Applied Animal Welfare Science 26(2), 218–228.

An optimized method for intratracheal instillation in mice

Non-invasive intratracheal instillation is an important method for direct exposure of the respiratory tract which is commonly used in toxicology, environmental science, and other research fields. However, there is no standard operating process for non-invasive intratracheal instillation. To keep the...

Year Published: 2022Topics: Disease/Experimental Model, Drug/Substance AdministrationAnimal Type: Mouse, Rodent

Citation: Zeng, Y., Jin, H., Wang, J. et al. 2022. An optimized method for intratracheal instillation in mice. Journal of Pharmacological and Toxicological Methods 118, 107230.

Evaluation of ambient sound, vibration, and light in rodent housing rooms

Excessive sound, vibration, and light are detrimental to rodent welfare, yet these parameters are rarely recorded in vivaria. Whether housing environments exceed the suggested thresholds and which specific factors may alter these parameters is generally unknown. The goal of this...

Year Published: 2022Topics: Husbandry & ManagementAnimal Type: Mouse, Rat, Rodent

Citation: Barabas, A. J., Darbyshire, A. K., Schlegel, S. L. et al. 2022. Evaluation of ambient sound, vibration, and light in rodent housing rooms. JAALAS 61(6), 660–671.