Fish

Anaesthesia and analgesia in laboratory adult zebrafish: a question of refinement

Anaesthesia is used daily in fish experimental procedures; however, the use of an inadequate anaesthetic protocol can compromise not only the animal's welfare but also the reliability of results. The use of zebrafish (Danio rerio) in biomedical research has increased...

Year Published: 2016Topics: Analgesia, Anesthesia & SedationAnimal Type: Fish, Zebrafish

Citation: Martins, T., Valentim, A. M., Pereira, L. N. et al. 2016. Anaesthesia and analgesia in laboratory adult zebrafish: a question of refinement. Laboratory Animals 50(6), 476-488.

The impact of social context on behaviour and the recovery from welfare challenges in zebrafish, Danio rerio

Understanding how animals experience stress in a laboratory environment is crucial for improving their welfare. Increasing numbers of fish are being used in scientific studies and further research is required to ensure appropriate conditions are used to promote good conduct...

Year Published: 2017Topics: Social Housing & Companionship, Welfare AssessmentAnimal Type: Fish, Zebrafish

Citation: White, L. J., Thomson, J. S., Pounder, K. C. et al. 2017. The impact of social context on behaviour and the recovery from welfare challenges in zebrafish, Danio rerio. Animal Behaviour 132, 189-199.

Reduction in activity by noxious chemical stimulation is ameliorated by immersion in analgesic drugs in zebrafish

Research has recently demonstrated that larval zebrafish show similar molecular responses to nociception to those of adults. Our study explored whether unprotected larval zebrafish exhibited altered behaviour after exposure to noxious chemicals and screened a range of analgesic drugs to...

Year Published: 2017Topics: Analgesia, Welfare AssessmentAnimal Type: Fish, Zebrafish

Citation: Lopez-Luna, J., Al-Jubouri, Q., Al-Nuaimy, W. et al. 2017. Reduction in activity by noxious chemical stimulation is ameliorated by immersion in analgesic drugs in zebrafish. Journal of Experimental Biology 220(8), 1451-1458.

Dried rotifer sheet: A novel live feed for rearing first-feeding larvae

Rotifers are now becoming a standard live feed for first-feeding larvae, because of their easy rearing protocol and improved growth and survival rate, compared with other fish feed, paramecia, and processed powder fish feeds. In a group of rotifers, bdelloid...

Year Published: 2018Topics: Husbandry & ManagementAnimal Type: Fish, Zebrafish

Citation: Nakayama, H., Katayama, K., Onabe, Y. et al. 2018. Dried rotifer sheet: A novel live feed for rearing first-feeding larvae. Zebrafish 15(3), 291–294.

Report of Workshop on Euthanasia for Zebrafish—A Matter of welfare and science

The increasing importance of zebrafish as a biomedical model organism is reflected by the steadily growing number of publications and laboratories working with this species. Regulatory recommendations for euthanasia as issued in Directive 2010/63/EU are, however, based on experience with...

Year Published: 2017Topics: EuthanasiaAnimal Type: Fish, Zebrafish

Citation: Köhler, A., Collymore, C., Finger-Baier, K. et al. 2017. Report of Workshop on Euthanasia for Zebrafish—A Matter of welfare and science. Zebrafish 14(6), 547-551.

Breeding zebrafish: A review of different methods and a discussion on standardization

In recent years, a rapidly increasing number of scientific papers have been published that utilize zebrafish (Danio rerio) as an alternative model organism in the study of a wide range of biological phenomena from cancer to behavior. This is, in...

Year Published: 2017Topics: Housing, Husbandry & Management, ReproductionAnimal Type: Fish, Zebrafish

Citation: Tsang, B., Zahid, H., Ansari, R. et al. 2017. Breeding zebrafish: A review of different methods and a discussion on standardization. Zebrafish 14(6), 561-573.

A low-cost method of skin swabbing for the collection of DNA samples from small laboratory fish

Fin clipping of live fish under anesthesia is widely used to collect samples for DNA extraction. An alternative, potentially less invasive, approach involves obtaining samples by swabbing the skin of nonanesthetized fish. However, this method has yet to be widely...

Year Published: 2017Topics: Identification MethodAnimal Type: Fish, Zebrafish

Citation: Breacker, C., Barber, I., Norton, W. H. J. et al. 2017. A low-cost method of skin swabbing for the collection of DNA samples from small laboratory fish. Zebrafish 14(1), 35-41.

Diet size preference of zebrafish (Danio rerio) larvae fed on cross-linked protein-walled capsules

To optimize diet particle size for ingestion by zebrafish larvae, Danio rerio, a series of diet selection experiments were carried out using two different length classes: 5-day-old, first feeding larvae with a mean standard length (MLs) of 3.8 mm and...

Year Published: 2016Topics: Husbandry & ManagementAnimal Type: Fish, Zebrafish

Citation: Önal, U., Langdon, C. 2016. Diet size preference of zebrafish (Danio rerio) larvae fed on cross-linked protein-walled capsules. Zebrafish 13(6), 556-562.

Color preference in Danio rerio: Effects of age and anxiolytic treatments

Zebrafish are increasingly used in neurobiological and behavioral studies. Possible stimuli to manipulate zebrafish behavior are being investigated. The presentation of colors appears to be one of the most used approaches, but there is much debate about the exact color...

Year Published: 2016Topics: HousingAnimal Type: Fish, Zebrafish

Citation: Peeters, B. W. M. M. , Moeskops, M., Veenvliet, A. R. J. 2016. Color preference in Danio rerio: Effects of age and anxiolytic treatments. Zebrafish 13(4), 330-334.

Evaluating and optimizing fish health and welfare during experimental procedures

Many facilities house fish in separate static containers post-procedure, for example, while awaiting genotyping results. This ensures fish can be easily identified, but it does not allow for provision of continuous filtered water or diet. At the Wellcome Trust Sanger...

Year Published: 2016Topics: Housing, Husbandry & ManagementAnimal Type: Fish, Zebrafish

Citation: Goodwin, N., Westall, L., Karp, N. A. et al. 2016. Evaluating and optimizing fish health and welfare during experimental procedures. Zebrafish 13(S1), S127-S131.