Fish

Do Buccal swabs from Zebrafish give enough of a sample of DNA to be used as a viable non-invasive method of genotyping? Animal Technology and Welfare 16(3), 155-164

The zebrafish has become a valuable research model within the laboratory. Currently the proven method of genotyping involves taking a tail fin clip from the fish to gain the sample. This involves surgically removing a piece of the tail fin...

Year Published: 2017Topics: Identification MethodAnimal Type: Fish, Zebrafish

Citation: Lawton, S. 2017. Do Buccal swabs from Zebrafish give enough of a sample of DNA to be used as a viable non-invasive method of genotyping? Animal Technology and Welfare 16(3), 155-164.

Use of tricaine methanesulfonate or propofol for immersion euthanasia of goldfish (Carassius auratus)

OBJECTIVE To substantiate current AVMA guidelines for immersion euthanasia of goldfish (Carassius auratus) with tricaine methanesulfonate (TMS), determine whether immersion in propofol at 5 times its immersion anesthesia concentration for 30 minutes is sufficient for euthanasia of goldfish, and quantify...

Year Published: 2018Topics: EuthanasiaAnimal Type: Fish, Other Fish

Citation: Balko, J. A., Oda, A., Posner, L. P. 2018. Use of tricaine methanesulfonate or propofol for immersion euthanasia of goldfish (Carassius auratus). Journal of the American Veterinary Medical Association 252(12), 1555-1561.

Intramuscular infiltration of a local anesthetic, lidocaine, does not result in adverse behavioural side effects in rainbow trout

Fish are a useful animal model for research, but our improvement in some aspects of their welfare has not kept pace with their increased popularity for this use. For example, researchers rarely use analgesics. We evaluated the side effects of...

Year Published: 2018Topics: Anesthesia & SedationAnimal Type: Fish, Trout

Citation: Chatigny, F., Creighton, C. M., Stevens, E. D. 2018. Intramuscular infiltration of a local anesthetic, lidocaine, does not result in adverse behavioural side effects in rainbow trout. Scientific Reports 8, 10250.

Fish sentience denial: Muddying the waters

Recent empirical studies have reported evidence that many aquatic species, including fish, cephalopods and crustaceans, have the capacity for nociception and pain, and that their welfare should be taken into consideration. Some sceptics, rejecting the precautionary principle, have denied that...

Year Published: 2018Topics: Welfare AssessmentAnimal Type: Fish, Zebrafish

Citation: Sneddon, L. U., Lopez-Luna, J., Wolfenden, D. et al. 2018. Fish sentience denial: Muddying the waters. Animal Sentience 21(1).

Uses and doses of local anesthetics in fish, amphibians, and reptiles

Local anesthetics are an integral part of routine pain management in mammals, yet their use is relatively limited in fish, amphibians and reptiles. These animals frequently undergo potentially painful surgical procedures and therefore could possibly benefit from those drugs. Some...

Year Published: 2017Topics: Analgesia, Anesthesia & SedationAnimal Type: Amphibian, Crocodile & Alligator, Fish, Frog & Toad, Lizard, Other Fish, Reptile, Salamander, Salmon, Snake, Tilapia, Trout, Turtle & Tortoise, Zebrafish

Citation: Chatigny, F., Kamunde, C., Creighton, C. M. et al. 2017. Uses and doses of local anesthetics in fish, amphibians, and reptiles. JAALAS 56(3), 244-253.

Behavioral characteristics of adult zebrafish (Danio rerio) after MS222 anesthesia for fin excision

The health of laboratory animals is an ethical responsibility of researchers and a critical determinant of experimental outcome. Therefore, all Husbandry & Management procedures should be evaluated for their effects on mortality, behavior, and physiology to maximize animal welfare and...

Year Published: 2017Topics: Anesthesia & SedationAnimal Type: Fish, Zebrafish

Citation: De Lombaert, M. C. M., Rick, E. L., Krugner-Higby, L. A. et al. 2017. Behavioral characteristics of adult zebrafish (Danio rerio) after MS222 anesthesia for fin excision. JAALAS 56(4), 377-381.

Updated review of fish analgesia

Analgesics are an integral part of routine pain management in mammals, yet their use in fish is still limited. Some recommendations on the use of analgesics in fish are currently in the literature; however, information on the properties of analgesic...

Year Published: 2018Topics: AnalgesiaAnimal Type: Fish, Other Fish, Salmon, Tilapia, Trout, Zebrafish

Citation: Chatigny, F., Creighton, C. M., Stevens, E. D. 2018. Updated review of fish analgesia. JAALAS 57(1), 5-12.

Effectiveness of rapid cooling as a method of euthanasia for young zebrafish (Danio rerio)

Despite increased use of zebrafish (Danio rerio) in biomedical research, consistent information regarding appropriate euthanasia methods, particularly for embryos, is sparse. Current literature indicates that rapid cooling is an effective method of euthanasia for adult zebrafish, yet consistent guidelines regarding...

Year Published: 2018Topics: EuthanasiaAnimal Type: Fish, Zebrafish

Citation: Wallace, C. K., Bright, L. A., Marx, J. O. et al. 2018. Effectiveness of rapid cooling as a method of euthanasia for young zebrafish (Danio rerio). JAALAS 57(1), 58-63.

A good death? Report of the second Newcastle meeting on laboratory animal euthanasia

Millions of laboratory animals are killed each year worldwide. There is an ethical, and in many countries also a legal, imperative to ensure those deaths cause minimal suffering. However, there is a lack of consensus regarding what methods of killing...

Year Published: 2016Topics: EuthanasiaAnimal Type: Fish, Mouse, Rat, Rodent, Zebrafish

Citation: Hawkins, P., Prescott, M., Carbone, L. et al. 2016. A good death? Report of the second Newcastle meeting on laboratory animal euthanasia. Animals 6(9), 50.

Anaesthesia and analgesia in laboratory adult zebrafish: a question of refinement

Anaesthesia is used daily in fish experimental procedures; however, the use of an inadequate anaesthetic protocol can compromise not only the animal's welfare but also the reliability of results. The use of zebrafish (Danio rerio) in biomedical research has increased...

Year Published: 2016Topics: Analgesia, Anesthesia & SedationAnimal Type: Fish, Zebrafish

Citation: Martins, T., Valentim, A. M., Pereira, L. N. et al. 2016. Anaesthesia and analgesia in laboratory adult zebrafish: a question of refinement. Laboratory Animals 50(6), 476-488.