Identification Method

An efficient, simple, and noninvasive procedure for genotyping aquatic and nonaquatic laboratory animals

Various animal models are indispensible in biomedical research. Increasing awareness and regulations have prompted the adaptation of more humane approaches in the use of laboratory animals. With the development of easier and faster methodologies to generate genetically altered animals, convenient...

Year Published: 2017Topics: Identification MethodAnimal Type: Amphibian, Frog & Toad, Mouse, Rodent

Citation: Okada, M., Miller, T. C., Roediger, J. et al. 2017. An efficient, simple, and noninvasive procedure for genotyping aquatic and nonaquatic laboratory animals. JAALAS 56(5), 570-573.

A systematic review of discomfort due to toe or ear clipping in laboratory rodents

Toe clipping and ear clipping (also ear notching or ear punching) are frequently used methods for individual identification of laboratory rodents. These procedures potentially cause severe discomfort, which can reduce animal welfare and distort experimental results. However, no systematic summary...

Year Published: 2017Topics: Identification MethodAnimal Type: Mouse, Rat, Rodent

Citation: Wever, K. E., Geessink, f. J., Brouwer, M. A. E. et al. 2017. A systematic review of discomfort due to toe or ear clipping in laboratory rodents. Laboratory Animals 51(6), 583-600.

A highly efficient strategy to determine genotypes of genetically-engineered mice using genomic DNA purified from hair roots

Genotyping of genetically-engineered mice is necessary for the effective design of breeding strategies and identification of mutant mice. This process relies on the identification of DNA markers introduced into genomic sequences of mice, a task usually performed using the polymerase...

Year Published: 2017Topics: Identification MethodAnimal Type: Mouse, Rodent

Citation: Otano-Rivera, V., Boakye, A., Grobe, N. et al. 2017. A highly efficient strategy to determine genotypes of genetically-engineered mice using genomic DNA purified from hair roots. Laboratory Animals 51(2), 138-146.

A low-cost method of skin swabbing for the collection of DNA samples from small laboratory fish

Fin clipping of live fish under anesthesia is widely used to collect samples for DNA extraction. An alternative, potentially less invasive, approach involves obtaining samples by swabbing the skin of nonanesthetized fish. However, this method has yet to be widely...

Year Published: 2017Topics: Identification MethodAnimal Type: Fish, Zebrafish

Citation: Breacker, C., Barber, I., Norton, W. H. J. et al. 2017. A low-cost method of skin swabbing for the collection of DNA samples from small laboratory fish. Zebrafish 14(1), 35-41.

An automated system for rapid cellular extraction from live zebrafish embryos and larvae: Development and application to genotyping

Zebrafish are a valuable model organism in biomedical research. Their rapid development, ability to model human diseases, utility for testing genetic variants identified from next-generation sequencing, amenity to CRISPR mutagenesis, and potential for therapeutic compound screening, has led to their...

Year Published: 2018Topics: Identification MethodAnimal Type: Fish, Zebrafish

Citation: Lambert, C. J., Freshner, B. C., Chung, A. et al. 2018. An automated system for rapid cellular extraction from live zebrafish embryos and larvae: Development and application to genotyping. PLOS ONE 13(3), e0193180.

Noninvasive genotyping of common marmoset (Callithrix jacchus) by fingernail PCR

The common marmoset (Callithrix jacchus) is a New World primate that is a useful model for medical studies. In this study, we report a convenient, reliable, and noninvasive procedure to genotype a living common marmoset by using fingernails. This method...

Year Published: 2015Topics: Identification MethodAnimal Type: Marmoset, Nonhuman Primate

Citation: Takabayashi, S., Katoh, H. 2015. Noninvasive genotyping of common marmoset (Callithrix jacchus) by fingernail PCR. Primates 56(3), 235-240.

Using the mouse grimace scale to assess pain associated with routine ear notching and the effect of analgesia in laboratory mice

Social housing is recommended where possible for laboratory mice. In order to achieve this, mice must be individually identifiable. Although, various methods are available, permanent identification is often required, such as ear notching. This method is likely to be painful...

Year Published: 2015Topics: Analgesia, Identification MethodAnimal Type: Mouse, Rodent

Citation: Miller, A. L., Leach, M. C. 2015. Using the mouse grimace scale to assess pain associated with routine ear notching and the effect of analgesia in laboratory mice. Laboratory Animals 49(2), 117-120.