Drug/Substance Administration

Evaluation of tissue changes following intramuscular infiltration of lidocaine in rainbow trout Oncorhynchus mykiss

Rainbow trout Oncorhynchus mykiss were infiltrated with either saline or lidocaine adjacent to the dorsal fin to assess histopathological changes. Infiltration was done as if it were being used as a local anaesthetic. Tissue lesions and associated tissue healing were...

Year Published: 2018Topics: Anesthesia & Sedation, Drug/Substance AdministrationAnimal Type: Fish, Trout

Citation: Chatigny, F., Groman, D. B., Martinson, S. A. et al. 2018. Evaluation of tissue changes following intramuscular infiltration of lidocaine in rainbow trout Oncorhynchus mykiss. Journal of Fish Biology 92(4), 888-900.

Assessment of pain associated with the injection of sodium pentobarbital in laboratory mice (Mus musculus)

The AVMA Guidelines for the Euthanasia of Animals considers injection of barbiturates to be an acceptable method of euthanasia in rodents but states there is a potential for pain when administered intraperitoneally. This study examined the potential for pain in...

Year Published: 2019Topics: Drug/Substance Administration, EuthanasiaAnimal Type: Mouse, Rodent

Citation: Dutton III, J. W., Artwohl, J. E., Huang, X. et al. 2019. Assessment of pain associated with the injection of sodium pentobarbital in laboratory mice (Mus musculus). JAALAS 58(3), 373-379.

Bottle or bowl? Feeding and dosing in juvenile Göttingen minipigs

The Laboratory Animal Technology department at Envigo were engaged to perform a toxicity study in juvenile Göttingen Minipigs. The test compound was a human milk formula additive. Therefore, to mimic the conditions of clinical administration, the dose was given in...

Year Published: 2019Topics: Drug/Substance AdministrationAnimal Type: Pig

Citation: McNamara, A., Allen, L. 2019. Bottle or bowl? Feeding and dosing in juvenile Göttingen minipigs. Animal Technology and Welfare 18(2), 153-154. (IAT Congress 2018 Poster Presentation)

Corticosterone assimilation by a voluntary oral administration in palatable food to rats

Drug delivery in research on nonhuman animals in the laboratory is still challenging because it is usually invasive and stressful. Stress-free voluntary oral drug administration in water lacks precise control of dose and timing of substance ingestion. Voluntary oral consumption...

Year Published: 2018Topics: Drug/Substance AdministrationAnimal Type: Rat, Rodent

Citation: Raya, J., Neves Girardi , C. E., Hipólide, D. C. 2019. Corticosterone assimilation by a voluntary oral administration in palatable food to rats. Journal of Applied Animal Welfare Science 22(1), 37-41.

Reducing the stress of drug administration: Implications for the 3Rs

Restraint in animals is known to cause stress but is used during almost all scientific procedures in rodents, representing a major welfare and scientific issue. Administration of substances, a key part of most scientific procedures, almost always involves physical restraint...

Year Published: 2015Topics: Drug/Substance Administration, RestraintAnimal Type: Rat, Rodent

Citation: Stuart, S., Robinson, E. S. J. 2015. Reducing the stress of drug administration: Implications for the 3Rs. Scientific Reports 5, 14288.

Administering fixed oral doses of curcumin to rats through voluntary consumption

Curcumin, a polyphenol derived from turmeric, has a wide variety of therapeutic benefits including antiinflammatory, antioxidative, and chemopreventative effects. Oral gavage is widely performed to administer curcumin in laboratory rodents in several experimental models. Although effective, this method can increase...

Year Published: 2018Topics: Drug/Substance AdministrationAnimal Type: Rat, Rodent

Citation: Hocking, A. J., Elliot, D., Hua, J. et al. 2018. Administering fixed oral doses of curcumin to rats through voluntary consumption. JAALAS 57(5), 508–512.

Going outside ASPA guidelines

This article will discuss an improved and refined technique used for working and caring for a group of diabetic mice. As a team of animal technologists, academics and named veterinary surgeon, we developed a new husbandry care regime to improve...

Year Published: 2016Topics: Disease/Experimental Model, Drug/Substance Administration, Environmental Enrichment, Husbandry & ManagementAnimal Type: Mouse, Rodent

Citation: Gardiner, P. 2016. Going outside ASPA guidelines. Animal Technology and Welfare 15(1), 47-52.

Refinement of tumour passage by optimisation of the tissue disaggregation protocol

Surgical implantation of tumour pieces has been used regularly in the passage of tumour models. This involves an invasive surgical procedure, carried out under anaesthesia, ensuring mice have adequate pain relief and peri-operative care. The procedure also requires multiple tumour-bearing...

Year Published: 2018Topics: Drug/Substance AdministrationAnimal Type: Mouse, Rodent

Citation: Galvin, M., Lallo, A., Brown, S. et al. 2018. Refinement of tumour passage by optimisation of the tissue disaggregation protocol. Animal Technology and Welfare 17(2), 101-103.

A refinement to Schedule 1

There are varying methods used for animal restraint and angle of injection. It remains unclear whether one method is more accurate than others. The primary objectives were to examine whether inverted restraint of rats resulted in more effective outcomes and...

Year Published: 2018Topics: Drug/Substance AdministrationAnimal Type: Rat, Rodent

Citation: Read, H., Perry, R. 2018. A refinement to Schedule 1. Animal Technology and Welfare 17(2), 117-120.

Design by intravenous injection in the Syrian hamster

Repeated injections in rats and mice are typically done via the tail vein. For hamsters, the tail is not an option. In this paper we explore the development and refinement of IV dosing in the Syrian hamster via the jugular...

Year Published: 2018Topics: Drug/Substance AdministrationAnimal Type: Hamster, Rodent

Citation: Beamish, G., Chapman, C., Lomax, A. 2018. Design by intravenous injection in the Syrian hamster. Animal Technology and Welfare 17(2), 124-125.