Drug/Substance Administration

Towards more translatable research: Exploring alternatives to gavage as the oral administration route of vaccines in rodents for improved animal welfare and human relevance

Oral vaccines are gaining attention for their ease of administration and ability to activate mucosal immune responses. However, preclinical studies using this route often require the use of gavage, which has limitations. In this comment, we briefly discuss the challenges...

Year Published: 2023Topics: Drug/Substance AdministrationAnimal Type: Mouse, Rat, Rodent

Citation: Miguelena Chamorro, B., Swaminathan, G., Mundt, E. et al. 2023. Towards more translatable research: Exploring alternatives to gavage as the oral administration route of vaccines in rodents for improved animal welfare and human relevance. Lab Animal 52(9), 195–197.

Mice just want to have fun: Playpens facilitate faster training in non-invasive techniques for improved refinement and well-being

Practical mechanisms that can be implemented by animal facilities to enhance refinement include the use of non-aversive handling, non-invasive alternative procedures, and additional environmental enrichment. Thus, we sought to collate these techniques by developing a protocol that utilizes positive reinforcement...

Year Published: 2023Topics: Animal Training, Drug/Substance AdministrationAnimal Type: Mouse, Rodent

Citation: Krall, C., Hopper, L. M., Hutchinson, E. K. 2023. Mice just want to have fun: Playpens facilitate faster training in non-invasive techniques for improved refinement and well-being. Laboratory Animal Science Professional 11(4) (July/August), 22-24.

A cost-effective restraint method for safer subcutaneous scruff injections and reducing stress in mice and handlers

Subcutaneous injection is a vital technique in mouse-based biomedical research. Compared to other injections, the subcutaneous method poses fewer technical challenges and reduces acute toxicity risks due to slower absorption. Several suitable subcutaneous injection sites exist in mice, including the...

Year Published: 2024Topics: Drug/Substance AdministrationAnimal Type: Mouse, Rodent

Citation: Fitzwater, A., Zhang, L. 2024. A cost-effective restraint method for safer subcutaneous scruff injections and reducing stress in mice and handlers. Laboratory Animal Science Professional 12(1) (January/February), 52-53.

Comparison of a minimally invasive transthoracic approach and a surgical method for intrapleural injection of tumor cells in mice

Intrapleural injections can be used in mice to deliver therapeutic and diagnostic agents and to model human disease processes (for example, pleural fluid accumulation, malignant pleural disease, and lung cancers). In the context of establishing cancer models, minimally invasive methods...

Year Published: 2023Topics: Disease/Experimental Model, Drug/Substance AdministrationAnimal Type: Mouse, Rodent

Citation: Xu, J. J., Lucero, M. Y., Herndon, N. L. et al. 2023. Comparison of a minimally invasive transthoracic approach and a surgical method for intrapleural injection of tumor cells in mice. Comparative Medicine 73(2), 120–126.

PiDose: An open-source system for accurate and automated oral drug administration to group-housed mice

Drug treatment studies in laboratory mice typically employ manual administration methods such as injection or gavage, which can be time-consuming to perform over long periods and cause substantial stress in animals. These stress responses may mask or enhance treatment effects,...

Year Published: 2020Topics: Drug/Substance AdministrationAnimal Type: Mouse, Rodent

Citation: Woodard, C. L., Nasrallah, W. B., Samiei, B. V. et al. 2020. PiDose: An open-source system for accurate and automated oral drug administration to group-housed mice. Scientific Reports 10, 11584.

Decreased levels of discomfort in repeatedly handled mice during experimental procedures, assessed by facial expressions

Mice are the most commonly used laboratory animal, yet there are limited studies which investigate the effects of repeated handling on their welfare and scientific outcomes. Furthermore, simple methods to evaluate distress in mice are lacking, and specialized behavioral or...

Year Published: 2023Topics: Animal Training, Drug/Substance Administration, Welfare AssessmentAnimal Type: Mouse, Rodent

Citation: Swan, J., Boyer, S., Westlund, K. et al. 2023. Decreased levels of discomfort in repeatedly handled mice during experimental procedures, assessed by facial expressions. Frontiers in Behavioral Neuroscience 17, 1109886.

Evaluation of parameters which influence voluntary ingestion of supplements in rats

Drug safety and efficacy studies frequently use oral gavage, but repetitive usage may cause problems. Administration through voluntary ingestion represents an opportunity for refinement. We aimed to develop a protocol for voluntary ingestion of gelatin-based supplements in rats, assessing the...

Year Published: 2023Topics: Animal Training, Drug/Substance AdministrationAnimal Type: Rat, Rodent

Citation: Ruvira, S., Rodríguez-Rodríguez, P., Cañas, S. et al. 2023. Evaluation of parameters which influence voluntary ingestion of supplements in rats. Animals 13(11), 1827.

Ultrasound guided surgery as a refinement tool in oncology research

Refinement is one of the ethical pillars of the use of animals in research. Ultrasonography is currently used in human medicine as a surgical tool for guided biopsies and this idea can be applied to preclinical research thanks to the...

Year Published: 2022Topics: Disease/Experimental Model, Drug/Substance Administration, Imaging & Electrophysiological Recording, Surgery & Post-OpAnimal Type: All/General

Citation: Serrano, C., Antonio, J. 2022. Ultrasound guided surgery as a refinement tool in oncology research. Animals 12(23), 3445.

Intranasal atomization of ketamine, medetomidine and butorphanol in pet rabbits using a mucosal atomization device

A non-invasive method of drug delivery, intranasal atomization, has shown positive results in human medicine and in some animal species. The objective of this study was to evaluate the effects of intranasal atomization, compared to intramuscular administration, of a mix...

Year Published: 2023Topics: Anesthesia & Sedation, Drug/Substance AdministrationAnimal Type: Rabbit

Citation: Mauthe von Degerfeld, M., Serpieri, M., Bonaffini, G. et al. 2023. Intranasal atomization of ketamine, medetomidine and butorphanol in pet rabbits using a mucosal atomization device. Animals 13(13), 2076.

Development of a 3D simulator for training the mouse in utero electroporation

In utero electroporation (IUE) requires high-level training in microinjection through the mouse uterine wall into the lateral ventricle of the mouse brain. Training for IUE is currently being performed in live mice as no artificial models allow simulations yet. This...

Year Published: 2022Topics: Disease/Experimental Model, Drug/Substance Administration, ReproductionAnimal Type: Mouse, Rodent

Citation: Nuber, M., Gonzalez-Uarquin, F., Neufurth, M. et al. 2022. Development of a 3D simulator for training the mouse in utero electroporation. PLOS ONE 17(12), e0279004.