Publications

Zebrafish maintenance conditions affect behavioral and biochemical biomarkers: A possible interfering factor on the research results

Over the years, scientific research with fish models has grown at a rapid pace, and issues such as animal welfare are becoming increasingly important in various areas of animal husbandry and experimentation. Here, we evaluated whether Danio rerio behavior is...

Year Published: 2024Topics: Environmental EnrichmentAnimal Type: Fish, Zebrafish

Citation: Amaral, F. U. I., Pompermaier, A., Almeida, P. A. de et al. 2024. Zebrafish maintenance conditions affect behavioral and biochemical biomarkers: A possible interfering factor on the research results. Zebrafish.

A bespoke water T–maze apparatus and protocol: An optimized, reliable, and repeatable method for screening learning, memory, and executive functioning in laboratory mice

The Morris Water Maze (MWM) is the most commonly used assay for evaluating learning and memory in laboratory mice. Despite its widespread use, contemporary reviews have highlighted substantial methodological variation in experimental protocols and that the associated testing procedures are...

Year Published: 2024Topics: Behavioral & Cognitive TestingAnimal Type: Mouse, Rodent

Citation: Bailoo, J. D., Bergeson, S. E., Ponomarev, I. et al. 2024. A bespoke water T–maze apparatus and protocol: An optimized, reliable, and repeatable method for screening learning, memory, and executive functioning in laboratory mice. Frontiers in Behavioral Neuroscience 18.

Think beyond the room: Measuring relative humidity in the home cage and its impact on reproduction in laboratory mice, mus musculus

Relative humidity (RH) is measured in vivaria with a broad range to accommodate seasonal fluctuations. It is assumed that measurements in the room (macroenvironment) reflect those in the cage (microenvironment). However, there is limited data comparing RH in the macroenvironment...

Year Published: 2024Topics: Husbandry & Management, ReproductionAnimal Type: Mouse, Rodent

Citation: Barabas, A. J., Conlon, R. A., Hodges, C. A. 2024. Think beyond the room: Measuring relative humidity in the home cage and its impact on reproduction in laboratory mice, mus musculus. Animals 14(22), 3164.

Report of the 2023 RSPCA/UFAW/IAT Rodent Welfare Group Meeting

This report summarises the meeting and covers discussions ranging from housing and husbandry to refinement in procedures, education, and learning from companion rate care. It includes some action points for readers to consider raising at their own establishments.

Year Published: 2024Topics: Drug/Substance Administration, Environmental Enrichment, Handling, Housing, Husbandry & ManagementAnimal Type: Mouse, Rat, Rodent

Citation: Barnabe, M., Applebee, K., Hurst, J. et al. 2024. Report of the 2023 RSPCA/UFAW/IAT Rodent Welfare Group Meeting. Animal Technology and Welfare 23(2), 81–90.

The 3Hs Initiative—Housing, handling, and habituation

We have looked at our housing, handling and habituation approaches to identify areas to make improvements. This is critical for our resaerch outputs and has the benefit of reducing the cumulative suffering the animals experience by reducing stress and negative...

Year Published: 2024Topics: Animal Training, Drug/Substance Administration, Handling, Housing, RestraintAnimal Type: Mouse, Rat, Rodent

Citation: Bartlett, J., Hinchcliffe, J., Jackson, M. et al. 2024. The 3Hs Initiative—Housing, handling, and habituation. Animal Technology and Welfare 23(2), 91–99.

Use of the oxygen reserve index/FiO2 as a non-invasive index to estimate venous admixture in anesthetized dogs

The oxygen reserve index (ORi) is a novel, non-invasive parameter that estimates arterial oxygen partial pressure (PaO2) during hyperoxia when the fraction of inspired oxygen (FiO2) is elevated. This study aimed to assess the utility of the ORi/FiO2 ratio as...

Year Published: 2025Topics: Anesthesia & Sedation, Biological Sampling & Physiological MeasurementAnimal Type: Dog

Citation: Bellini, L., Maney, J. K., Zanusso, F. et al. 2025. Use of the oxygen reserve index/FiO2 as a non-invasive index to estimate venous admixture in anesthetized dogs. Frontiers in Veterinary Science 11.

The effect of preference assessment-informed enrichment device colour on biting and foraging behaviour in loggerhead sea turtles

Although environmental enrichment procedures have demonstrated their benefit to non-human animals in zoos and aquariums, the field has given little attention to phylogenetic variables that might affect their efficacy. Recently, research with loggerhead sea turtles Caretta caretta has demonstrated that...

Year Published: 2024Topics: Environmental EnrichmentAnimal Type: Reptile, Turtle & Tortoise

Citation: Bulla, A., Mahoney, A., Lee, V. M. et al. 2024. The effect of preference assessment-informed enrichment device colour on biting and foraging behaviour in loggerhead sea turtles. Journal of Zoo and Aquarium Research 12(4), 232–242.

What are the priority welfare issues facing parrots in captivity? A modified Delphi approach to establish expert consensus

Parrots (Psittaciformes) are widely kept in captivity, yet their welfare is under-researched in comparison to other captive species. This study aimed to determine key welfare issues affecting parrots through a modified Delphi approach. Twenty-eight welfare issues were first compiled via...

Year Published: 2024Topics: Abnormal/Problematic Behavior, Environmental Enrichment, Housing, Social Housing & CompanionshipAnimal Type: Bird, Parrot

Citation: Chalmers, R., Cooper, J., Ventura, B. 2024. What are the priority welfare issues facing parrots in captivity? A modified Delphi approach to establish expert consensus. Animal Welfare 33, e54.

Evaluating enrichment use in group-housed rhesus macaques (Macaca mulatta): A machine learning approach

Environmental enrichment programmes are widely used to improve welfare of captive and laboratory animals, especially non-human primates. Monitoring enrichment use over time is crucial, as animals may habituate and reduce their interaction with it. In this study we aimed to...

Year Published: 2024Topics: Environmental EnrichmentAnimal Type: Macaque, Nonhuman Primate

Citation: Ciminelli, G., Witham, C., Bateson, M. 2024. Evaluating enrichment use in group-housed rhesus macaques (Macaca mulatta): A machine learning approach. Animal Welfare 33, e59.

Early-life enrichment in American mink (Neogale vison): Effects of juvenile physical enrichment on behaviour, temperament, and long-term stereotypic behaviour

A single manipulable enrichment is often introduced to the pens of farmed American mink (Neogale vison) to combat stereotypic behaviour and behaviours or temperaments associated with poor welfare (e.g. inactivity, fear, and aggression). This enrichment is provided early in life,...

Year Published: 2025Topics: Environmental Enrichment, Rearing & WeaningAnimal Type: Mink

Citation: Clark, G. B., Díez-León, M., Meagher, R. K. 2025. Early-life enrichment in American mink (Neogale vison): Effects of juvenile physical enrichment on behaviour, temperament, and long-term stereotypic behaviour. Animal Welfare 34, e4.