Rodent

Application of the 3R principles: Vertebrae as an additional source of murine bone-marrow cells

Experimental murine models are an essential tool in the field of bone marrow (BM) transplantation research. Therefore, numerous mice are required to obtain a sufficient number of BM cells, which is in contrast with the Reduction principle of the 3R...

Year Published: 2021Topics: Disease/Experimental ModelAnimal Type: Mouse, Rodent

Citation: Justin, M., Jež, M., Košir, A. et al. 2021. Application of the 3R principles: Vertebrae as an additional source of murine bone-marrow cells. Laboratory Animals 55(1), 43-52.

An alternative method for oral drug administration by voluntary intake in male and female mice

Drug administration to experimental rodents is often invasive and stressful, thus reducing animal welfare and potentially confounding experimental results. Methods of oral drug delivery in which rodents cooperate voluntarily minimize stress, pain and morbidity. We herein describe a method for oral...

Year Published: 2021Topics: Drug/Substance AdministrationAnimal Type: Mouse, Rodent

Citation: Teixeira-Santos, L., Albino-Teixeira, A., Pinho, D. 2021. An alternative method for oral drug administration by voluntary intake in male and female mice. Laboratory Animals 55(1), 76-80.

Drip, drop…drip, drop – How do I make it stop?

Whether you are an animal care/husbandry technician, facility manager, or veterinarian, everyone bemoans a leaky drinking valve (often referred to as a lixit). Leaking drinking valves and flooded cages are a fairly common problem when using automatic watering systems (or...

Year Published: 2021Topics: Husbandry & ManagementAnimal Type: All/General, Mouse, Rat, Rodent

Citation: Webb, L. 2021. Drip, drop…drip, drop – How do I make it stop? Laboratory Animal Science Professional 9(2) (March/April), 36-38.

Wellbeing of mice euthanized with carbon dioxide in their home cage as compared with an induction chamber

The AVMA Guidelines on Euthanasia state that, to decrease potential distress of animals, the home cage should be used for the euthanasia of mice. The current study evaluated this recommendation by comparing behavioral and physiologic changes in ICR and SJL...

Year Published: 2021Topics: EuthanasiaAnimal Type: Mouse, Rodent

Citation: Hickman, D. L. 2021. Wellbeing of mice euthanized with carbon dioxide in their home cage as compared with an induction chamber. JAALAS 60(1), 72-76.

Effects of compressed paper bedding on mouse breeding performance and recognition of animal health concerns

The combination of bedding substrate and nesting material within the microenvironment of mice is an important consideration for animal care programs in regard to optimizing animal wellbeing. We used 3 general or breeding mouse colonies in our institution to evaluate...

Year Published: 2021Topics: Husbandry & Management, Welfare AssessmentAnimal Type: Mouse, Rodent

Citation: Allen, P. S., Lawrence, J., Stasula, U. et al. 2021. Effects of compressed paper bedding on mouse breeding performance and recognition of animal health concerns. JAALAS 60(1), 28-36.

Comparison of four beddings for ammonia control in individually ventilated mouse cages

Ammonia control is an important characteristic of rodent bedding materials. Among natural bedding materials, corncob bedding provides excellent ammonia control but contains estrogenic compounds and is ingested by mice. By comparison, processed cellulose bedding products are biologically inert and harbor...

Year Published: 2021Topics: Husbandry & ManagementAnimal Type: Mouse, Rodent

Citation: Tataryn, N. M., Buckmaster, C. A., Schwiebert, R. et al. 2021. Comparison of four beddings for ammonia control in individually ventilated mouse cages. JAALAS 60(1), 37-43.

Effects of acrylic tunnel enrichment on anxiety-like behavior, neurogenesis, and physiology of C57BL/6J mice

Environmental enrichment for mice lags behind the standard enrichment offered to other laboratory rodents due to concerns about environmental variability and, in specific contexts, aggression. Our objective in this study was to evaluate concerns that the introduction of structural enrichment...

Year Published: 2021Topics: Environmental EnrichmentAnimal Type: Mouse, Rodent

Citation: Oatess, T. L., Harrison, F. E., Himmel, L. E. et al. 2021. Effects of acrylic tunnel enrichment on anxiety-like behavior, neurogenesis, and physiology of C57BL/6J mice. JAALAS 60(1), 44-53.

Anesthetic effects of alfaxalone–ketamine, alfaxalone–ketamine–dexmedetomidine, and alfaxalone–butorphanol–midazolam administered intramuscularly in five striped palm squirrels (Funambulus pennantii)

Injectable anesthesia protocols for five striped palm squirrels (Funambulus pennantii) are poorly described in the literature. In this study, male intact squirrels received intramuscular injections of either alfaxalone (6 mg/kg) and ketamine (40 mg/ kg; AK group, n = 8);...

Year Published: 2020Topics: Anesthesia & SedationAnimal Type: Other Rodent, Rodent

Citation: Eshar, D., Beaufrère, H. 2020. Anesthetic effects of alfaxalone–ketamine, alfaxalone–ketamine–dexmedetomidine, and alfaxalone–butorphanol–midazolam administered intramuscularly in five striped palm squirrels (Funambulus pennantii). JAALAS 59(4), 384-392.

Compressed paper as an alternative to corn cob bedding in mouse (Mus musculus) cages

Bedding material is a critical component of the mouse environment and affects animal wellbeing and research integrity. Corn cob (CC) bedding has been a common bedding choice in research despite several potential negative aspects of its use. We investigated the...

Year Published: 2020Topics: Husbandry & ManagementAnimal Type: Mouse, Rodent

Citation: Pallas, B. D., Keys, D. M., Bradley, M. P. et al. 2020. Compressed paper as an alternative to corn cob bedding in mouse (Mus musculus) cages. JAALAS 59(5), 496-502.

A comparison of ketamine or etomidate combined with xylazine for intraperitoneal anesthesia in four mouse strains

Intraperitoneal (IP) injection is a common route of anesthetic administration in mice. Ketamine-xylazine (KX) anesthesia is one of the most widely used IP protocols, but has limitations. Etomidate is an alternative to ketamine that has been used in both human...

Year Published: 2020Topics: Anesthesia & SedationAnimal Type: Mouse, Rodent

Citation: Gergye, C. H., Zhao, Y., Moore, R. et al. 2020. A comparison of ketamine or etomidate combined with xylazine for intraperitoneal anesthesia in four mouse strains. JAALAS 59(5), 519-530.