Rodent

Buprenorphine, but not lidocaine, effectively attenuates post-operative thermal hypersensitivity in an incisional model in neonatal rats (Rattus norvegicus)

There is limited information on safe and effective neonatal rodent analgesia. The aim of this study was to evaluate the efficacy and duration of analgesia provided by buprenorphine (Bup) and lidocaine (Lid) in an incisional pain model. Male and female...

Year Published: 2021Topics: Analgesia, Surgery & Post-OpAnimal Type: Rat, Rodent

Citation: Katz, E. M., Huss, M. K., Jampachaisri, K. et al. 2021. Buprenorphine, but not lidocaine, effectively attenuates post-operative thermal hypersensitivity in an incisional model in neonatal rats (Rattus norvegicus). Scandinavian Journal of Laboratory Animal Science 47(1), 1-11.

Estimating mouse and rat use in American laboratories by extrapolation from Animal Welfare Act-regulated species

Alone among Western nations, the United States has a two-tier system for welfare protections for vertebrate animals in research. Because its Animal Welfare Act (AWA) excludes laboratory rats and mice (RM), government veterinarians do not inspect RM laboratories and RM...

Year Published: 2021Topics: Regulations & Ethical ReviewAnimal Type: Mouse, Rat, Rodent

Citation: Carbone, L. 2021. Estimating mouse and rat use in American laboratories by extrapolation from Animal Welfare Act-regulated species. Scientific Reports 11(1), 493.

Pharmacokinetics and efficacy of a long-lasting, highly concentrated buprenorphine solution in mice

Buprenorphine is a commonly used opioid for mitigating pain in laboratory mice after surgical procedures; however, the dosing interval necessary for standard buprenorphine may require treatment every 4 to 6 h to maintain an adequate plane of analgesia. An alternative...

Year Published: 2021Topics: AnalgesiaAnimal Type: Mouse, Rodent

Citation: Kendall, L. V., Singh, B., Bailey, A. L. et al. 2021. Pharmacokinetics and efficacy of a long-lasting, highly concentrated buprenorphine solution in mice. JAALAS 60(1), 64-71.

Replacement of surgical vasectomy through the use of wild-type sterile hybrids

For the production and rederivation of mouse strains, pseudopregnant female mice are used for embryo transfer and serve as surrogate mothers to support embryo development to term. Vasectomized males are commonly used to render pseudopregnancy in females, generated by surgical...

Year Published: 2021Topics: ReproductionAnimal Type: Mouse, Rodent

Citation: Preece, C., Alghadban, S., Bouchareb, A. et al. 2021. Replacement of surgical vasectomy through the use of wild-type sterile hybrids. Lab Animal 50, 49-52.

Oral application of clozapine-N-oxide using the micropipette-guided drug administration (MDA) method in mouse DREADD systems

The designer receptor exclusively activated by designer drugs (DREADD) system is one of the most widely used chemogenetic techniques to modulate the activity of cell populations in the brains of behaving animals. DREADDs are activated by acute or chronic administration...

Year Published: 2021Topics: Drug/Substance AdministrationAnimal Type: Mouse, Rodent

Citation: Schalbetter, S. M., Mueller, F. S., Scarborough, J. et al. 2021. Oral application of clozapine-N-oxide using the micropipette-guided drug administration (MDA) method in mouse DREADD systems. Lab Animal 50, 69-75.

Refining the unilateral ureteral obstruction mouse model: No sham, no shame

Fibrosis, as a common final pathway in many renal diseases, contributes significantly to the decline of organ function and to progression to end-stage renal disease. To establish therapeutic interventions that target fibrosis, animal models are essential. The most widely used...

Year Published: 2021Topics: Disease/Experimental ModelAnimal Type: Mouse, Rodent

Citation: Atkinson, J., Boden, T., Mocho J.-P. et al. 2021. Refining the unilateral ureteral obstruction mouse model: No sham, no shame. Laboratory Animals 55(1), 21-29.

Application of the 3R principles: Vertebrae as an additional source of murine bone-marrow cells

Experimental murine models are an essential tool in the field of bone marrow (BM) transplantation research. Therefore, numerous mice are required to obtain a sufficient number of BM cells, which is in contrast with the Reduction principle of the 3R...

Year Published: 2021Topics: Disease/Experimental ModelAnimal Type: Mouse, Rodent

Citation: Justin, M., Jež, M., Košir, A. et al. 2021. Application of the 3R principles: Vertebrae as an additional source of murine bone-marrow cells. Laboratory Animals 55(1), 43-52.

An alternative method for oral drug administration by voluntary intake in male and female mice

Drug administration to experimental rodents is often invasive and stressful, thus reducing animal welfare and potentially confounding experimental results. Methods of oral drug delivery in which rodents cooperate voluntarily minimize stress, pain and morbidity. We herein describe a method for oral...

Year Published: 2021Topics: Drug/Substance AdministrationAnimal Type: Mouse, Rodent

Citation: Teixeira-Santos, L., Albino-Teixeira, A., Pinho, D. 2021. An alternative method for oral drug administration by voluntary intake in male and female mice. Laboratory Animals 55(1), 76-80.

Drip, drop…drip, drop – How do I make it stop?

Whether you are an animal care/husbandry technician, facility manager, or veterinarian, everyone bemoans a leaky drinking valve (often referred to as a lixit). Leaking drinking valves and flooded cages are a fairly common problem when using automatic watering systems (or...

Year Published: 2021Topics: Husbandry & ManagementAnimal Type: All/General, Mouse, Rat, Rodent

Citation: Webb, L. 2021. Drip, drop…drip, drop – How do I make it stop? Laboratory Animal Science Professional 9(2) (March/April), 36-38.

Wellbeing of mice euthanized with carbon dioxide in their home cage as compared with an induction chamber

The AVMA Guidelines on Euthanasia state that, to decrease potential distress of animals, the home cage should be used for the euthanasia of mice. The current study evaluated this recommendation by comparing behavioral and physiologic changes in ICR and SJL...

Year Published: 2021Topics: EuthanasiaAnimal Type: Mouse, Rodent

Citation: Hickman, D. L. 2021. Wellbeing of mice euthanized with carbon dioxide in their home cage as compared with an induction chamber. JAALAS 60(1), 72-76.