Rodent

High laboratory mouse pre-weaning mortality associated with litter overlap, advanced dam age, small and large litters

High and variable pre-weaning mortality is a persistent problem in laboratory mouse breeding. Assuming a modest 15% mortality rate across mouse strains, means that approximately 1 million more pups are produced yearly in the EU to compensate for those which...

Year Published: 2020Topics: Husbandry & Management, Rearing & WeaningAnimal Type: Mouse, Rodent

Citation: Morello, G. M., Hultgren, J., Capas-Peneda, S. et al. 2020. High laboratory mouse pre-weaning mortality associated with litter overlap, advanced dam age, small and large litters. PLOS ONE 15(8): e0236290.

Housing behaviour of the naked mole rat (Heterocephalus glaber) under laboratory conditions

The naked mole rat (Heterocephalus glaber) is a rodent that has gained importance as a biomedical research model for various conditions including hypoxic brain injury, cancer and nociception. It is captured from the wild and housed under laboratory conditions during...

Year Published: 2020Topics: Housing, Natural BehaviorAnimal Type: Mole Rat, Rodent

Citation: Mwobobia, R., Abelson, K., Kanui, T. 2020. Housing behaviour of the naked mole rat (Heterocephalus glaber) under laboratory conditions. Scandinavian Journal of Laboratory Animal Science 46(1), 16-24.

Novel bleeding techniques in hamsters

Hamsters have historically been used in our pharmacokinetic (PK) studies using the retro-orbital (RO) bleeding technique to collect blood samples. If performed incorrectly, this technique has the potential for animal welfare complications not usually seen with other phlebotomy methods. Our...

Year Published: 2020Topics: Biological Sampling & Physiological MeasurementAnimal Type: Hamster, Rodent

Citation: Filliettaz, C., Maxwell, L., Dragon, M. et al. 2020. Novel bleeding techniques in hamsters. Laboratory Animal Science Professional 8(1) (January/February), 44-46.

Oestrus checking – increasing productivity and embracing the 3R’s

Much like the menstrual cycle in humans, mice also have a cycle where the eggs ripen, ovulation takes place, the females come into heat and are receptive to mate with the male; this occurs over the space of 4-5 days...

Year Published: 2020Topics: ReproductionAnimal Type: Mouse, Rodent

Citation: Hoskins, S., Brown, J. 2020. Oestrus checking – increasing productivity and embracing the 3R’s. Animal Technology and Welfare 19(2), 159-161.

Optimising streptozotocin dosing to minimise renal toxicity and impairment of stomach emptying in male 129/Sv mice

The streptozotocin (STZ)-induced diabetic mouse model has been extensively used as a model for diabetes and diabetic nephropathy, but it is still influenced by many off-target toxic effects and large variation in diabetes induction. Therefore, the aim of this study...

Year Published: 2020Topics: Disease/Experimental ModelAnimal Type: Mouse, Rodent

Citation: Nørgaard, S. A., Søndergaard, H., Sørensen, D. B. et al. 2020. Optimising streptozotocin dosing to minimise renal toxicity and impairment of stomach emptying in male 129/Sv mice. Laboratory Animals 54(4), 341–352.

Performance of severity parameters to detect chemotherapy-induced pain and distress in mice

According to European Union directive 2010/63/EU a severity classification of experimental procedures performed on laboratory animals is mandatory. This includes a prospective evaluation of all interventions performed within the experiment, as well as an assessment of the actual burden of...

Year Published: 2020Topics: Disease/Experimental Model, Welfare AssessmentAnimal Type: Mouse, Rodent

Citation: Rix, A., Drude, N., Mrugalla, A. et al. 2020. Performance of severity parameters to detect chemotherapy-induced pain and distress in mice. Laboratory Animals 54(5), 452-460.

Progressing the care, husbandry and management of ageing mice used in scientific studies

Driven by the longer lifespans of humans, particularly in Westernised societies, and the need to know more about ‘healthy ageing’, ageing mice are being used increasingly in scientific research. Many departments and institutes involved with ageing research have developed their...

Year Published: 2020Topics: Disease/Experimental Model, Humane Endpoint, Welfare AssessmentAnimal Type: Mouse, Rodent

Citation: Wilkinson, M. J. A., Selman, C., McLaughlin, L. et al. 2020. Progressing the care, husbandry and management of ageing mice used in scientific studies. Laboratory Animals 54(3) 225–238.

Protection of blood-brain barrier as a potential mechanism for enriched environments to improve cognitive impairment caused by chronic cerebral hypoperfusion

Chronic cerebral hypoperfusion (CCH) is a common pathophysiological basis for Alzheimer’s Disease and vascular dementia in the early stages. It has been confirmed that blood-brain barrier (BBB) destruction is a key factor in CCH-related cognitive impairment. Here we explored the...

Year Published: 2020Topics: Environmental EnrichmentAnimal Type: Rat, Rodent

Citation: Qu, C., Xu, L., Shen, J. et al. 2020. Protection of blood-brain barrier as a potential mechanism for enriched environments to improve cognitive impairment caused by chronic cerebral hypoperfusion. Behavioural Brain Research 379, 112385.

Manganese-free chow, a refined non-invasive solution to reduce gastrointestinal signal for T1-weighted magnetic resonance imaging of the mouse abdomen

Commercial mouse chow is designed to provide a complete, nutrient-rich diet, and it can contain upwards of 100 mg/kg manganese, an essential mineral. Manganese acts as a relaxation time-shortening contrast agent for both T1 and T2, and where standard chow is...

Year Published: 2020Topics: Husbandry & ManagementAnimal Type: Mouse, Rodent

Citation: Kersemans, V., Wallington, S., Allen, P. D. et al. 2020. Manganese-free chow, a refined non-invasive solution to reduce gastrointestinal signal for T1-weighted magnetic resonance imaging of the mouse abdomen. Laboratory Animals 54(4), 353–364.

Maternal deprivation impairs memory and cognitive flexibility, effect that is avoided by environmental enrichment

Maternal deprivation (MD) causes cognitive deficits that persist until adulthood. Thereby, the environmental enrichment (EE) is widely used to increase brain plasticity. Here, pregnant female rats were used and their offspring were submitted to neonatal MD from post-natal day 1–10;...

Year Published: 2020Topics: Environmental EnrichmentAnimal Type: Rat, Rodent

Citation: Menezes, J., Souto das Neves, B.-H., Gonçalves, R. et al. 2020. Maternal deprivation impairs memory and cognitive flexibility, effect that is avoided by environmental enrichment. Behavioural Brain Research 381, 112468.