Mouse

Body temperature measurement in mice during acute illness: Implantable temperature transponder versus surface infrared thermometry

Body temperature is a valuable parameter in determining the wellbeing of laboratory animals. However, using body temperature to refine humane endpoints during acute illness generally lacks comprehensiveness and exposes to inter-observer bias. Here we compared two methods to assess body...

Year Published: 2018Topics: Biological Sampling & Physiological Measurement, Welfare AssessmentAnimal Type: Mouse, Rodent

Citation: Mei, J., Riedel, N., Grittner, U. et al. 2018. Body temperature measurement in mice during acute illness: Implantable temperature transponder versus surface infrared thermometry. Scientific Reports 8, 3526.

Comparison of atipamezole with yohimbine for antagonism of xylazine in mice anesthetized with ketamine and xylazine

The α2 adrenergic agonist xylazine produces a sedative effect and is typically combined with ketamine and used for anesthesia or chemical restraint of laboratory mice. Xylazine's sedative effect—and its undesirable side effects of bradycardia, hypotension, and poor tissue perfusion—can be...

Year Published: 2017Topics: Anesthesia & SedationAnimal Type: Mouse, Rodent

Citation: Janssen, C. F., Maiello, P., Wright Jr, M. J. et al. 2017. Comparison of atipamezole with yohimbine for antagonism of xylazine in mice anesthetized with ketamine and xylazine. JAALAS 56(2), 142-147.

Benefits of 21% oxygen compared with 100% oxygen for delivery of isoflurane to mice (Mus musculus) and rats (Rattus norvegicus)

At research institutions, isoflurane delivered by precision vaporizer to a face mask is the standard for rodent surgery and for procedures with durations that exceed a few minutes. Pure oxygen is often used as the carrier gas for isoflurane anesthesia,...

Year Published: 2017Topics: Anesthesia & SedationAnimal Type: Mouse, Rat, Rodent

Citation: Wilding, L. A., Hampel, J. A., Khoury, B. M. et al. 2017. Benefits of 21% oxygen compared with 100% oxygen for delivery of isoflurane to mice (Mus musculus) and rats (Rattus norvegicus). JAALAS 56(2), 148-154.

A device that allows rodents to behaviorally thermoregulate when housed in vivariums

Laboratories and vivariums typically are maintained at ambient temperatures of 20 to 24 °C, leading to cold stress in mice. When mice are inactive and sleeping during the light phase, their zone of thermoneutrality associated with a basal metabolic rate...

Year Published: 2017Topics: Husbandry & ManagementAnimal Type: Mouse, Rat, Rodent

Citation: Gordon, C. J., Puckett, E. T., Repasky, E. S. et al. 2017. A device that allows rodents to behaviorally thermoregulate when housed in vivariums. JAALAS 56(2), 173-176.

What goes around can come around: An unexpected deleterious effect of using mouse running wheels for environmental enrichment

Environmental enrichment items such as running wheels can promote the wellbeing of laboratory mice. Growing evidence suggests that wheel running simulates exercise effects in many mouse models of human conditions, but this activity also might change other aspects of mouse...

Year Published: 2017Topics: Environmental EnrichmentAnimal Type: Mouse, Rodent

Citation: Leduc, R. Y. M., Rauw, G., Baker, G. B. et al. 2017. What goes around can come around: An unexpected deleterious effect of using mouse running wheels for environmental enrichment. JAALAS 56(2), 194-201.

Physiologic, behavioral, and histologic responses to various euthanasia methods in C57BL/6NTac male mice

Rodent euthanasia using exposure to increasing concentrations of CO2 has come under scrutiny due to concerns of potential pain during the euthanasia process. Alternatives to CO2, such as isoflurane and barbiturates, have been proposed as more humane methods of euthanasia....

Year Published: 2017Topics: EuthanasiaAnimal Type: Mouse, Rodent

Citation: Boivin, G. P., Bottomley, M. A., Schiml, P. A. et al. 2017. Physiologic, behavioral, and histologic responses to various euthanasia methods in C57BL/6NTac male mice. JAALAS 56(1), 69-78.

Efficacy of sustained-release buprenorphine in an experimental laparotomy model in female mice

Mice purportedly require dosing with the opioid buprenorphine (Bup-HCl) at least every 8 to 12 h to maintain an adequate plane of analgesia. Here we used an experimental laparotomy model to determine the clinical efficacy of sustained-release formulations of buprenorphine...

Year Published: 2016Topics: AnalgesiaAnimal Type: Mouse, Rodent

Citation: Kendall, L. V., Wegenast, D. J., Smith, B. J. et al. 2016. Efficacy of sustained-release buprenorphine in an experimental laparotomy model in female mice. JAALAS 55(1), 66–73.

Effects of nesting material on energy homeostasis in BALB/cAnNCrl, C57BL/6NCrl, and Crl:CD1(ICR) mice housed at 20 °C

Discrepancies exist between the preferred temperature range for mice (26 to 32 °C) and current recommendations (20 to 26 °C), which may alter metabolism and negatively affect studies using mice. Previous research indicates that nesting material can alleviate cold stress...

Year Published: 2017Topics: Environmental EnrichmentAnimal Type: Mouse, Rodent

Citation: Johnson, J. S., Taylor, D. J., Green, A. R. et al. 2017. Effects of nesting material on energy homeostasis in BALB/cAnNCrl, C57BL/6NCrl, and Crl:CD1(ICR) mice housed at 20 °C. JAALAS 56(3), 254-259.

Intraperitoneal administration of ethanol as a means of euthanasia for neonatal mice (Mus musculus)

The humane euthanasia of animals in research is of paramount importance. Neonatal mice frequently respond differently to euthanasia agents when compared with adults. The AVMA's Guidelines for the Euthanasia of Animals includes intraperitoneal injection of ethanol as "acceptable with conditions,"...

Year Published: 2017Topics: EuthanasiaAnimal Type: Mouse, Rodent

Citation: de Souza Dyer, C., Brice, A. K., Marx, J. O. 2017. Intraperitoneal administration of ethanol as a means of euthanasia for neonatal mice (Mus musculus). JAALAS 56(3), 299-306.

Comparing phlebotomy by tail tip amputation, facial vein puncture, and tail vein incision in C57BL/6 mice by using physiologic and behavioral metrics of pain and distress

Tail tip amputation with minimal restraint is not widely used for mouse phlebotomy. In part, this infrequency may reflect policies influenced by tail tip amputation procedures for genotyping, which involve greater handling and tissue removal. To assess tail tip amputation...

Year Published: 2017Topics: Biological Sampling & Physiological MeasurementAnimal Type: Mouse, Rodent

Citation: Moore, E. S., Cleland, T. A., Williams, W. O. et al. 2017. Comparing phlebotomy by tail tip amputation, facial vein puncture, and tail vein incision in C57BL/6 mice by using physiologic and behavioral metrics of pain and distress. JAALAS 56(3), 307-317.