Mouse

A new tool for quantifying mouse facial expressions

Facial expressions are an increasingly used tool to assess emotional experience and affective state during experimental procedures in animal models. Previous studies have successfully related specific facial features with different positive and negative valence situations, most notably in relation to...

Year Published: 2023Topics: Emotion, Pain, & Sentience, Welfare AssessmentAnimal Type: Mouse, Rodent

Citation: Moëne, O. L., Larsson, M. 2023. A new tool for quantifying mouse facial expressions. ENeuro 10(2) ENEURO.0349-22.2022.

Conventional laboratory housing increases morbidity and mortality in research rodents: Results of a meta-analysis

Over 120 million mice and rats are used annually in research, conventionally housed in shoebox-sized cages that restrict natural behaviours (e.g. nesting and burrowing). This can reduce physical fitness, impair thermoregulation and reduce welfare (e.g. inducing abnormal stereotypic behaviours). In...

Year Published: 2022Topics: Disease/Experimental Model, Environmental Enrichment, HousingAnimal Type: Mouse, Rat, Rodent

Citation: Cait, J., Cait, A., Scott, R. W. et al. 2022. Conventional laboratory housing increases morbidity and mortality in research rodents: Results of a meta-analysis. BMC Biology 20(1), 15.

Decreased levels of discomfort in repeatedly handled mice during experimental procedures, assessed by facial expressions

Mice are the most commonly used laboratory animal, yet there are limited studies which investigate the effects of repeated handling on their welfare and scientific outcomes. Furthermore, simple methods to evaluate distress in mice are lacking, and specialized behavioral or...

Year Published: 2023Topics: Animal Training, Drug/Substance Administration, Welfare AssessmentAnimal Type: Mouse, Rodent

Citation: Swan, J., Boyer, S., Westlund, K. et al. 2023. Decreased levels of discomfort in repeatedly handled mice during experimental procedures, assessed by facial expressions. Frontiers in Behavioral Neuroscience 17, 1109886.

RELSA—A multidimensional procedure for the comparative assessment of well-being and the quantitative determination of severity in experimental procedures

Good science in translational research requires good animal welfare according to the principles of 3Rs. In many countries, determining animal welfare is a mandatory legal requirement, implying a categorization of animal suffering, traditionally dominated by subjective scorings. However, how such...

Year Published: 2022Topics: Welfare AssessmentAnimal Type: Mouse, Rodent

Citation: Talbot, S. R., Struve, B., Wassermann, L. et al. 2022. RELSA—A multidimensional procedure for the comparative assessment of well-being and the quantitative determination of severity in experimental procedures. Frontiers in Veterinary Science 9, 937711.

Minimising aggression in CD-1 and CD-1 background male mice with different enrichment types

Cage-mate aggression is a well-known problem that is more prevalent in certain strains of mice and is one of the most common reasons for single housing, increased stress levels, premature death and euthanasia. Numerous methods can be implemented to ameliorate...

Year Published: 2023Topics: Environmental Enrichment, Husbandry & Management, Social Housing & CompanionshipAnimal Type: Mouse, Rodent

Citation: Veness, A., Coyle, C., Murphy, S. et al. 2023. Minimising aggression in CD-1 and CD-1 background male mice with different enrichment types. Animal Technology and Welfare 22(1), 74–76.

Are mirrors aversive or rewarding for mice? Insights from the mirror preference test

Research investigating the effects of mirror exposure in mice found that the presence of mirrors has similar effects to the presence of cage mates. Restraint in a small holder induces hyperthermia (stress-induce hyperthermia: SIH) in mice but a restrained mouse...

Year Published: 2023Topics: Behavioral & Cognitive Testing, Environmental EnrichmentAnimal Type: Mouse, Rodent

Citation: Watanabe, S. 2023. Are mirrors aversive or rewarding for mice? Insights from the mirror preference test. Frontiers in Behavioral Neuroscience 17, 1137206.

Infrared thermography for non-invasive measurement of social inequality aversion in rodents and potential usefulness for future animal-friendly studies

Infrared thermography is a method that detects thermal radiation energy and can measure the body surface temperature of animals from a distance. While rectal temperature has traditionally been used to measure animals' core temperature, thermal imaging can avoid the stress...

Year Published: 2023Topics: Biological Sampling & Physiological Measurement, Emotion, Pain, & SentienceAnimal Type: Mouse, Rodent

Citation: Watanabe, S. 2023. Infrared thermography for non-invasive measurement of social inequality aversion in rodents and potential usefulness for future animal-friendly studies. Frontiers in Behavioral Neuroscience 17, 1131427.

A buprenorphine depot formulation provides effective sustained post-surgical analgesia for 72 h in mouse femoral fracture models

Adequate pain management is essential for ethical and scientific reasons in animal experiments and should completely cover the period of expected pain without the need for frequent re-application. However, current depot formulations of Buprenorphine are only available in the USA...

Year Published: 2023Topics: Analgesia, Disease/Experimental ModelAnimal Type: Mouse, Rodent

Citation: Wolter, A., Bucher, C. H., Kurmies, S. et al. 2023. A buprenorphine depot formulation provides effective sustained post-surgical analgesia for 72 h in mouse femoral fracture models. Scientific Reports 13(1), 3824.

Validating in-cage mouse enrichment

There is a wide variety of different types of mouse enrichment currently available, some items of which are more practical than others. Validating items used for providing enrichment can be time consuming and costly, nevertheless it is important to ensure...

Year Published: 2023Topics: Environmental EnrichmentAnimal Type: Mouse, Rodent

Citation: Burbidge, C., Beresford, Z., Serrano-Galleg, V. 2023. Validating in-cage mouse enrichment. Animal Technology and Welfare 22(1), 63–67.

The power of a touch: Regular touchscreen training but not its termination affects hormones and behavior in mice

Touchscreen-based procedures are increasingly used in experimental animal research. They not only represent a promising approach for translational research, but have also been highlighted as a powerful tool to reduce potential experimenter effects in animal studies. However, to prepare the...

Year Published: 2023Topics: Animal Training, Behavioral & Cognitive Testing, Environmental EnrichmentAnimal Type: Mouse, Rodent

Citation: Quante, S. M., Siewert, V., Palme, R. et al. 2023. The power of a touch: Regular touchscreen training but not its termination affects hormones and behavior in mice. Frontiers in Behavioral Neuroscience 17, 1112780.