Mouse

Hands-on refinements: Establishing improved husbandry processes for aged C57BL/6 mice

The main aim of this project was to review the husbandry processes in place for the colony of aged C57BL/6 mice at the Babraham Institute, establish a refined husbandry process and improve local guidance. Secondly, by analysing fate and clinical...

Year Published: 2024Topics: Husbandry & Management, Welfare AssessmentAnimal Type: Mouse, Rodent

Citation: Paterson, A. 2024. Hands-on refinements: Establishing improved husbandry processes for aged C57BL/6 mice. Animal Technology and Welfare 23(2), 129–131.

Non-invasive sampling methods for genotyping: Improvements towards the 4r principle

In research involving genetically modified mice which is usually to perform genotyping using invasive ear or tail biopsies in 92% of cases, there is a growing shift towards non-invasive sampling techniques such as oral swabs, hair, faeces and tears. Our...

Year Published: 2024Topics: Biological Sampling & Physiological Measurement, Identification MethodAnimal Type: Mouse, Rabbit, Rodent

Citation: Thasian-Sivarajah, S., Mohr, R., Walter, M. et al. 2024. Non-invasive sampling methods for genotyping: Improvements towards the 4r principle. Animal Technology and Welfare 23(2), 133–136.

Testing perioperative meloxicam analgesia to enhance welfare while preserving model validity in an inflammation-induced seizure model

Despite the international effort to improve laboratory animal welfare through the 3R principles (Reduce, Refine, Replace), many scientists still fail to implement and report their assessment of pain and well-being, likely due to concerns regarding the potential effects of analgesics...

Year Published: 2024Topics: Analgesia, Disease/Experimental Model, Surgery & Post-OpAnimal Type: Mouse, Rodent

Citation: Weiß, E., Pauletti, A., Egilmez, A. et al. 2024. Testing perioperative meloxicam analgesia to enhance welfare while preserving model validity in an inflammation-induced seizure model. Scientific Reports 14(1), 30563.

A new approach (CSA-Score) to assessing the cumulative overall stress load in the case of animal experiments and even beyond

In laboratory animal science, severity assessment has been mandatory by European law since 2013. Various severity assessments have been created for many single manipulations. However, when multiple harmful interventions or stressors occur simultaneously or repeatedly, the cumulative overall severity is...

Year Published: 2024Topics: Welfare AssessmentAnimal Type: All/General, Mouse, Pig, Rodent

Citation: Helmig, S., Wenzel, S. 2024. A new approach (CSA-Score) to assessing the cumulative overall stress load in the case of animal experiments and even beyond. Discover Animals 1(1), 38.

Ultrasound to ultrasee—The use of ultrasound to determine pregnancy in mice

Several MRC Laboratory of Molecular Biology (LMB) groups require early-stage mouse embryos for their scientific research programmes. Traditionally, Animal Technicians have relied on visual checks or palpation of the abdomen to determine pregnancy in mice. In 2017 an ultrasound machine...

Year Published: 2024Topics: Imaging & Electrophysiological Recording, ReproductionAnimal Type: Mouse, Rodent

Citation: Karam, C., Kemp, K. 2024. Ultrasound to ultrasee—The use of ultrasound to determine pregnancy in mice. Animal Technology and Welfare 23(2), 121–127.

Do we still need a canary in the coal mine for laboratory animal facilities? A systematic review of environmental health monitoring versus soiled bedding sentinels

Introduction Despite increasing evidence that Environmental Health Monitoring (EHM) is an effective method to perform rodent colony health surveillance, promotes the 3Rs as a Replacement, is comparable or reduces cost, and demonstrates labor benefits, many research institutions continue to use...

Year Published: 2024Topics: Husbandry & Management, Welfare AssessmentAnimal Type: Mouse, Rat, Rodent

Citation: LaFollette, M. R., Clement, C. S., Luchins, K. R. et al. 2024. Do we still need a canary in the coal mine for laboratory animal facilities? A systematic review of environmental health monitoring versus soiled bedding sentinels. PLOS ONE 19(12), e0311840.

Social contagion of pain and fear results in opposite social behaviors in rodents: Meta- analysis of experimental studies

The study aimed to explore the key factors influencing emotional valence in rodents, focusing on the critical elements that distinguish the contagion processes of fear and pain.MethodsThrough a systematic review and meta-analysis, we examined behavioral outcomes of rodents exposed to...

Year Published: 2024Topics: Emotion, Pain, & Sentience, Social Housing & CompanionshipAnimal Type: Mouse, Rat, Rodent

Citation: Du, R., Yu, Y., Wang, X.-L. et al. 2024. Social contagion of pain and fear results in opposite social behaviors in rodents: Meta- analysis of experimental studies. Frontiers in Behavioral Neuroscience 18.

A bespoke water T–maze apparatus and protocol: An optimized, reliable, and repeatable method for screening learning, memory, and executive functioning in laboratory mice

The Morris Water Maze (MWM) is the most commonly used assay for evaluating learning and memory in laboratory mice. Despite its widespread use, contemporary reviews have highlighted substantial methodological variation in experimental protocols and that the associated testing procedures are...

Year Published: 2024Topics: Behavioral & Cognitive TestingAnimal Type: Mouse, Rodent

Citation: Bailoo, J. D., Bergeson, S. E., Ponomarev, I. et al. 2024. A bespoke water T–maze apparatus and protocol: An optimized, reliable, and repeatable method for screening learning, memory, and executive functioning in laboratory mice. Frontiers in Behavioral Neuroscience 18.

Think beyond the room: Measuring relative humidity in the home cage and its impact on reproduction in laboratory mice, mus musculus

Relative humidity (RH) is measured in vivaria with a broad range to accommodate seasonal fluctuations. It is assumed that measurements in the room (macroenvironment) reflect those in the cage (microenvironment). However, there is limited data comparing RH in the macroenvironment...

Year Published: 2024Topics: Husbandry & Management, ReproductionAnimal Type: Mouse, Rodent

Citation: Barabas, A. J., Conlon, R. A., Hodges, C. A. 2024. Think beyond the room: Measuring relative humidity in the home cage and its impact on reproduction in laboratory mice, mus musculus. Animals 14(22), 3164.

Report of the 2023 RSPCA/UFAW/IAT Rodent Welfare Group Meeting

This report summarises the meeting and covers discussions ranging from housing and husbandry to refinement in procedures, education, and learning from companion rate care. It includes some action points for readers to consider raising at their own establishments.

Year Published: 2024Topics: Drug/Substance Administration, Environmental Enrichment, Handling, Housing, Husbandry & ManagementAnimal Type: Mouse, Rat, Rodent

Citation: Barnabe, M., Applebee, K., Hurst, J. et al. 2024. Report of the 2023 RSPCA/UFAW/IAT Rodent Welfare Group Meeting. Animal Technology and Welfare 23(2), 81–90.