Mouse

Mouse strain-specific habituation to oral metamizole administration

When pain might occur during an animal experiment, sufficient analgesia is necessary. Metamizole is the third most used postoperative pain medication in animal research. The analgesic effect of metamizole is supposed to last 6–8 h in rodents. Therefore, the supplementation of...

Year Published: 2025Topics: Drug/Substance AdministrationAnimal Type: Mouse, Rodent

Citation: Schreiber, T., Leitner, E., Brandstetter, J. et al. 2025. Mouse strain-specific habituation to oral metamizole administration. Laboratory Animals 59(2), 192–202.

Breeding planning for laboratory mice

In laboratory animal facilities, due to the rules of mammalian genetics, animals are often produced that do not possess the required genotype for planned experiments. To minimize the number of these animals that cannot be used for the intended research,...

Year Published: 2025Topics: ReproductionAnimal Type: Mouse, Rodent

Citation: Schenkel, J., Nagel-Riedasch, S., Zevnik, B. et al. 2025. Breeding planning for laboratory mice. Laboratory Animals 59(3), 415–415.

Current practices of pain assessment and analgesic use in laboratory mice: A 2022 FELASA Working Group survey

Assessing and alleviating pain in animals involved in research is critically important. However, the effective implementation of pain management depends on the knowledge and attitudes of the personnel involved. Following a Federation of European Laboratory Animal Science Associations ‘Pain in...

Year Published: 2025Topics: Analgesia, Welfare AssessmentAnimal Type: Mouse, Rodent

Citation: Gomez de Segura, I. A., Seeldrayers, S., Flecknell, P. 2025. Current practices of pain assessment and analgesic use in laboratory mice: A 2022 FELASA Working Group survey. Laboratory Animals 59(3), 396–412.

Mouse aversion to induction with isoflurane using the drop method

Isoflurane anesthesia prior to carbon dioxide euthanasia is recognized as a refinement by many guidelines. Facilities lacking access to a vaporizer can use the “drop” method, whereby liquid anesthetic is introduced into an induction chamber. Knowing the least aversive concentration...

Year Published: 2025Topics: EuthanasiaAnimal Type: Mouse, Rodent

Citation: Bodnar, M. J., Makowska, I. J., Boyd, C. T. et al. 2025. Mouse aversion to induction with isoflurane using the drop method. Laboratory Animals 59(2), 169–177.

Environmental enrichment attenuates reproductive adversity in a mouse model of Parkinson disease

Environmental enrichment is the provision of different substrates to mimic an animal’s natural environment and encourage natural, species-specific behavior. However, the use of enrichment to improve breeding efficiency in mouse models for neurologic conditions is not well described. There are...

Year Published: 2025Topics: Environmental Enrichment, ReproductionAnimal Type: Mouse, Rodent

Citation: Walsh, C. A., Johnson, L., Cirillo, P. A. et al. 2025. Environmental enrichment attenuates reproductive adversity in a mouse model of parkinson disease. Journal of the American Association for Laboratory Animal Science 64(1), 58–63.

Hypothermia as a sole euthanasia method for neonatal mice (Mus musculus)

Per the AVMA euthanasia guidelines, there are no data supporting the use of hypothermia as a solitary method of euthanasia for neonatal mice. However, the use of a secondary physical method of euthanasia, such as decapitation, could be time-consuming and...

Year Published: 2025Topics: EuthanasiaAnimal Type: Mouse, Rodent

Citation: Tang, N.-K., Theil, J. H., Oates, R. S. 2025. Hypothermia as a sole euthanasia method for neonatal mice (Mus musculus). Journal of the American Association for Laboratory Animal Science 64(3), 500–506.

Gavage-needle voluntary consumption administration of a dose-specific measure to mice (Mus musculus)

Orogastric gavage is a common technique used to administer test articles to rodents with risks ranging from increased stress to death of the animal. In this study, we propose a novel technique to administer treatments to mice for voluntary consumption...

Year Published: 2025Topics: Drug/Substance AdministrationAnimal Type: Mouse, Rodent

Citation: Punger, E. M., Norris, S. L., Stevens, S. C. et al. 2025. Gavage-needle voluntary consumption administration of a dose-specific measure to mice (Mus musculus). Journal of the American Association for Laboratory Animal Science 64(2), 287–293.

Opinion: Ethical challenges in depression research: The tail suspension test, the forced swim test, and alternative behavioral models

Behavioral tests in laboratory animals, particularly rodents, are considered vital for understanding the mechanisms underlying depression and evaluating potential treatments. However, ethical concerns regarding the use of traditional methods, such as the tail suspension test and the forced swimming test,...

Year Published: 2025Topics: Behavioral & Cognitive TestingAnimal Type: Mouse, Rat, Rodent

Citation: Mohseni, F., Rafaiee, R. 2025. Opinion: Ethical challenges in depression research: The tail suspension test, the forced swim test, and alternative behavioral models. Journal of the American Association for Laboratory Animal Science 64(3), 352–355.

Effect of novel high-fat diet feeding methods on food wastage, weight gain, hair coat grease accumulation, and scratching behavior in C57BL/6NCrl mice

Soft-pelleted, high-fat diets (HFD) are greasy and crumble easily leading to food wastage and hair coat grease accumulation when mice are fed using commercially available feeders. The ideal HFD feeder design should reduce food wastage, facilitate mouse weight gain, and...

Year Published: 2025Topics: Husbandry & ManagementAnimal Type: Mouse, Rodent

Citation: Guy, A. R., Klores, M., Prestia, K. et al. 2025. Effect of novel high-fat diet feeding methods on food wastage, weight gain, hair coat grease accumulation, and scratching behavior in C57BL/6NCrl mice. Journal of the American Association for Laboratory Animal Science 64(1), 98–105.

Behavioral or nutritional drive: Which motivation affects rates of food grinding in CD1 mice?

Wire mouse feeders used in laboratory mouse cages typically hold enough food to feed 5 mice for two weeks. However, some mice gnaw the food provided into powder, which they do not ingest. The ground-up food fills the cage, resulting...

Year Published: 2025Topics: Abnormal/Problematic Behavior, Husbandry & ManagementAnimal Type: Mouse, Rodent

Citation: Gaskill, B. N., Davis, H., Gosselin, R. P. et al. 2025. Behavioral or nutritional drive: Which motivation affects rates of food grinding in CD1 mice? Applied Animal Behaviour Science 284, 106533.