Imaging & Electrophysiological Recording

A fine-scale and minimally invasive marking method for use with conventional tungsten microelectrodes

In neurophysiology, achieving precise correlation between physiological responses and anatomic structures is a significant challenge. Therefore, the accuracy of the electrode marking method is crucial. In this study, we describe a tungsten-deposition method, in which tungsten oxide is generated by...

Year Published: 2023Topics: Imaging & Electrophysiological RecordingAnimal Type: Macaque, Mouse, Nonhuman Primate, Rodent

Citation: Oikawa, T., Nomura, K., Hara, T. et al. 2023. A fine-scale and minimally invasive marking method for use with conventional tungsten microelectrodes. eNeuro 10(9), ENEURO.0141-23.2023.

Sedline® miscalculation of depth of anaesthesia variables in two pigs due to electrocardiographic signal contamination

Two young (11-week-old) pigs underwent sole propofol anaesthesia as part of an experimental study. The depth of anaesthesia was evaluated both clinically and using the electroencephalography(EEG)-based monitor Sedline; in particular, the patient state index, suppression ratio, raw EEG traces, and...

Year Published: 2023Topics: Anesthesia & Sedation, Imaging & Electrophysiological RecordingAnimal Type: Pig

Citation: Mirra, A., Hight, D., Kovacevic, A. et al. 2023. Sedline® miscalculation of depth of anaesthesia variables in two pigs due to electrocardiographic signal contamination. Animals 13(17), 2699.

Home-enclosure-based behavioral and wireless neural recording setup for unrestrained rhesus macaques

Electrophysiological studies with behaving nonhuman primates often require the separation of animals from their social group as well as partial movement restraint to perform well-controlled experiments. When the research goal per se does not mandate constraining the animals' movements, there...

Year Published: 2023Topics: Imaging & Electrophysiological RecordingAnimal Type: Macaque, Nonhuman Primate

Citation: Hansmeyer, L., Yurt, P., Agha, N. et al. 2023. Home-enclosure-based behavioral and wireless neural recording setup for unrestrained rhesus macaques. ENeuro 10(1) ENEURO.0285-22.2022.

Ultrasound guided surgery as a refinement tool in oncology research

Refinement is one of the ethical pillars of the use of animals in research. Ultrasonography is currently used in human medicine as a surgical tool for guided biopsies and this idea can be applied to preclinical research thanks to the...

Year Published: 2022Topics: Disease/Experimental Model, Drug/Substance Administration, Imaging & Electrophysiological Recording, Surgery & Post-OpAnimal Type: All/General

Citation: Serrano, C., Antonio, J. 2022. Ultrasound guided surgery as a refinement tool in oncology research. Animals 12(23), 3445.

Longitudinal studies on Alzheimer disease mouse models with multiple tracer PET/CT: Application of reduction and refinement principles in daily practice to safeguard animal welfare during progressive aging

Longitudinal studies on mouse models related to Alzheimer disease (AD) pathology play an important role in the investigation of therapeutic targets to help pharmaceutical research in the development of new drugs and in the attempt of an early diagnosis that...

Year Published: 2023Topics: Disease/Experimental Model, Husbandry & Management, Imaging & Electrophysiological RecordingAnimal Type: Mouse, Rodent

Citation: Palumbo, G., Kunze, L. H., Oos, R. et al. 2023. Longitudinal studies on Alzheimer disease mouse models with multiple tracer PET/CT: Application of reduction and refinement principles in daily practice to safeguard animal welfare during progressive aging. Animals 13(11), 1812.

Using bioluminescence to image gene expression and spontaneous behavior in freely moving mice

Bioluminescence imaging (BLI) of gene expression in live animals is a powerful method for monitoring development, tumor growth, infections, healing, and other progressive, long-term biological processes. BLI remains an effective approach for reducing the number of animals needed to monitor...

Year Published: 2023Topics: Imaging & Electrophysiological RecordingAnimal Type: Mouse, Rodent

Citation: Malik, A., Zavadil, J. A., Geusz, M. E. 2023. Using bioluminescence to image gene expression and spontaneous behavior in freely moving mice. PLOS ONE 18(1), e0279875.

Probing microstructural changes in muscles of leptin-deficient zebrafish by non-invasive ex-vivo magnetic resonance microimaging

Leptin is a hormone that plays a key role in controlling food intake and energy homeostasis. Skeletal muscle is an important target for leptin and recent studies have shown that leptin deficiency may lead to muscular atrophy. However, leptin deficiency-induced...

Year Published: 2023Topics: Disease/Experimental Model, Imaging & Electrophysiological RecordingAnimal Type: Fish, Zebrafish

Citation: Eeza, M. N. H., Singer, R., Ding, Y. et al. 2023. Probing microstructural changes in muscles of leptin-deficient zebrafish by non-invasive ex-vivo magnetic resonance microimaging. PLOS ONE 18(4), e0284215.

Impact of partial bile duct ligation with or without repeated magnetic resonance imaging examinations in mice

Partial bile duct ligation (pBDL) is considered a well-tolerated cholestatic model. Magnetic resonance imaging (MRI) is one of the most widely used tools in noninvasive imaging. However, no systematic studies have reported the possible effects of repeated MRI assessments in...

Year Published: 2022Topics: Disease/Experimental Model, Imaging & Electrophysiological RecordingAnimal Type: Mouse, Rodent

Citation: Chen, T., Zhou, Z.-Y., Liu, J.-Y. et al. 2022. Impact of partial bile duct ligation with or without repeated magnetic resonance imaging examinations in mice. Scientific Reports 12(1), 21014.

Five hot topics in refinement of nonhuman primate neuroscience research

Members of the North American 3Rs Collaborative (NA3RsC) refinement initiative have chosen to summarize developments in five hot topics of interest: 1. Tips for welfare-friendly transport, chairing, and restraint; 2. Guidance on refining food and fluid control; 3. Protective caps...

Year Published: 2022Topics: Cannulation, Catheterization, & Intubation, Handling, Husbandry & Management, Imaging & Electrophysiological Recording, Relocation & Transport, RestraintAnimal Type: Macaque, Marmoset, Nonhuman Primate

Citation: Buckmaster, C. L., Levesque, D., LaFollette, M. et al. 2022. Five hot topics in refinement of nonhuman primate neuroscience research. Laboratory Animal Science Professional 10(1) (January/February), 24-26.

Refined acquisition of high-resolution chest CTs in macaques by free breathing

The use of medical imaging as a non-invasive or minimally invasive method to assess disease or treatment response continues to grow. A similar trend is observed in pre-clinical research, in general, and more specifically in macaques, enabling longitudinal assessment of...

Year Published: 2021Topics: Imaging & Electrophysiological RecordingAnimal Type: Macaque, Nonhuman Primate

Citation: Tölgyesi, B., Bakker, J., Nagy, K. et al. 2021. Refined acquisition of high-resolution chest CTs in macaques by free breathing. Laboratory Animals 55(6), 551-559.