Identification Method

Non-invasive sampling methods for genotyping: Improvements towards the 4r principle

In research involving genetically modified mice which is usually to perform genotyping using invasive ear or tail biopsies in 92% of cases, there is a growing shift towards non-invasive sampling techniques such as oral swabs, hair, faeces and tears. Our...

Year Published: 2024Topics: Biological Sampling & Physiological Measurement, Identification MethodAnimal Type: Mouse, Rabbit, Rodent

Citation: Thasian-Sivarajah, S., Mohr, R., Walter, M. et al. 2024. Non-invasive sampling methods for genotyping: Improvements towards the 4r principle. Animal Technology and Welfare 23(2), 133–136.

Standard methods for marking caudate amphibians do not impair animal welfare over the short term: An experimental approach

Major advancements in ecology and biodiversity conservation have been made thanks to methods for marking and individually tracking animals. Marking animals is both widely used and controversial due to the potential consequences for animal welfare, which are often incompletely evaluated...

Year Published: 2024Topics: Handling, Identification MethodAnimal Type: Amphibian, Salamander

Citation: Daversa, D. R., Baxter, E., Rosa, G. M. et al. 2024. Standard methods for marking caudate amphibians do not impair animal welfare over the short term: An experimental approach. Animal Welfare 33, e24.

A 3D-printed dummy for training distal phalanx amputation in mice

The development of realistic dummies for training the distal phalanx amputation (DPA) technique in mouse pups is a promising alternative to reduce and replace animals in training for research and teaching. To test this, we obtained micro-CT data from postnatal...

Year Published: 2024Topics: Identification MethodAnimal Type: Mouse, Rodent

Citation: Heuser, M., Gonzalez-Uarquin, F., Nuber, M. et al. 2024. A 3D-printed dummy for training distal phalanx amputation in mice. Animals 14(8), 1253.

An overview of black tree monitor (Varanus beccarii) husbandry

This article describes the enclosure, environmental parameters, feeding, enclosure dynamics, breeding, handling, identification, and enrichment for zoo-housed black tree monitors.

Year Published: 2023Topics: Environmental Enrichment, Handling, Housing, Husbandry & Management, Identification Method, Reproduction, Social Housing & CompanionshipAnimal Type: Lizard, Reptile

Citation: Newton, R. 2023. An overview of black tree monitor (Varanus beccarii) husbandry. RATEL (Journal of the Association of British and Irish Wild Animal Keepers) 50(3-4), 24-25.

Pinna edge biopsy of 7 and 21 day old C57BL/6 mice as a method for identification and genotyping

Identifying and genotyping mice prior to weaning can be useful for mouse colony management. Mice of an undesired genotype can be identified prior to weaning and removed from further study, resulting in a reduction of housing costs, and labor time....

Year Published: 2023Topics: Identification MethodAnimal Type: Mouse, Rodent

Citation: Chen, D. D., Molk, D. M., Palley, L. S. et al. 2023. Pinna edge biopsy of 7 and 21 day old C57BL/6 mice as a method for identification and genotyping. JAALAS 62(5), 438–448.

Dog identification method based on muzzle pattern image

Currently, invasive and external radio frequency identification (RFID) devices and pet tags are widely used for dog identification. However, social problems such as abandoning and losing dogs are constantly increasing. A more effective alternative to the existing identification method is...

Year Published: 2020Topics: Identification MethodAnimal Type: Dog

Citation: Jang, D.-H., Kwon, K.-S., Kim, J.-K. et al. 2020. Dog identification method based on muzzle pattern image. Applied Sciences 10(24), 8994.

Refinement of animal experiments: Replacing traumatic methods of laboratory animal marking with non-invasive alternatives

Reliable methods for identifying rodents play an important role in ensuring the success of preclinical studies. However, animal identification remains a trivial laboratory routine that is not often discussed, despite the fact that more than 6 million rodents are used...

Year Published: 2023Topics: Identification MethodAnimal Type: Mouse, Rat, Rodent

Citation: Klabukov, I., Shestakova, V., Krasilnikova, O. et al. 2023. Refinement of animal experiments: Replacing traumatic methods of laboratory animal marking with non-invasive alternatives. Animals 13(22), 3452.

Evaluation of healing progression at surgical incision sites and the use of antiseptics for enhancing post-operative survival in subyearling Chinook salmon (Oncorhynchus tshawytscha)

In an attempt to develop more effective surgical implantation methods for fish, surgical incisions typical of those made for implanting micro-acoustic transmitters into the peritoneal cavity were evaluated on a weekly basis for healing progression using a suite of metrics....

Year Published: 2023Topics: Identification Method, Surgery & Post-OpAnimal Type: Fish, Salmon

Citation: Cameron, A. S., Rub, A. M. W., Sandford, B. P. 2023. Evaluation of healing progression at surgical incision sites and the use of antiseptics for enhancing post-operative survival in subyearling Chinook salmon (Oncorhynchus tshawytscha). PLOS ONE 18(7), e0288056.

Image-based automatic individual identification of fish without obvious patterns on the body (scale pattern)

The precision fish farming concept has been widely investigated in research and is highly desirable in aquaculture as it creates opportunities for precisely controlling and monitoring fish cultivation processes and increasing fish welfare. The automatic identification of individual fish could...

Year Published: 2022Topics: Identification MethodAnimal Type: Fish, Other Fish

Citation: Bekkozhayeva, D., Cisar, P. 2022. Image-based automatic individual identification of fish without obvious patterns on the body (scale pattern). Applied Sciences 12(11), 5401.

Tetra-primer amplification-refractory mutationsystem (ARMS)—PCR for genotyping mouse leptin gene mutation

Due to spontaneous deficiency in leptin, ob/ob mice are one of the most commonly used experimental animal models in diabetes research. In this study, we reported a quick and easy-to-conduct genotyping method using tetra-primer amplification refractory mutation system-polymerase chain reaction...

Year Published: 2022Topics: Disease/Experimental Model, Identification MethodAnimal Type: Mouse, Rodent

Citation: Chen, J., Xu, X., Dalhaimer, P. et al. 2022. Tetra-primer amplification-refractory mutationsystem (ARMS)—PCR for genotyping mouse leptin gene mutation. Animals 12(19), 2680.