Biological Sampling & Physiological Measurement

Differences in fluid, electrolyte, and energy balance in C57BL/6J mice (Mus musculus) in metabolic caging at thermoneutral or standard room temperatures

The Guide for the Care and Use of Laboratory Animals recommends mice be pair or group housed and provided with nesting materials. These provisions support social interactions and are also critical for thermoregulatory behaviors such as huddling and burrowing. However,...

Year Published: 2024Topics: Biological Sampling & Physiological Measurement, Husbandry & ManagementAnimal Type: Mouse, Rodent

Citation: Lawton, S. B., Grobe, C. C., Reho, J. J. et al. 2024. Differences in fluid, electrolyte, and energy balance in C57BL/6J mice (Mus musculus) in metabolic caging at thermoneutral or standard room temperatures. JAALAS 63(2), 190–200.

Clean-catching urine from pigs: A method for collecting quality specimens for urinalysis and microbiological culturing in a laboratory environment

Urine is an important biological specimen for assessing various metabolic functions and drug clearance. In urinary tract infection research, urine is particularly important as urinary bacterial titres constitute the main diagnostic outcome for assessing the course of infection. Collecting uncontaminated...

Year Published: 2023Topics: Biological Sampling & Physiological MeasurementAnimal Type: Pig

Citation: Stærk, K., Langhorn, L., Andersen, T. E. 2023. Clean-catching urine from pigs: A method for collecting quality specimens for urinalysis and microbiological culturing in a laboratory environment. Laboratory Animals 57(3), 293–303.

Noninvasive monitoring of blood pressure and heart rate during estrous cycle phases in bormotensive Wistar–Kyoto and spontaneously hypertensive female rats

Since 2015, the National Institutes of Health has called for its funded preclinical research to include both male and female subjects. However, much of the basic animal research that has studied heart rate and blood pressure in the past has...

Year Published: 2023Topics: Biological Sampling & Physiological MeasurementAnimal Type: Rat, Rodent

Citation: Ayala-Méndez, G. X., Calderón, V. M., Zuñiga-Pimentel, T. A. et al. 2023. Noninvasive monitoring of blood pressure and heart rate during estrous cycle phases in bormotensive Wistar–Kyoto and spontaneously hypertensive female rats. JAALAS 62(3), 267–273.

Evaluation of submental blood collection in mice (Mus musculus)

The submental route is an option for nonterminal and serial blood collection in mice. This study compared the submental route to the maxillary route (also referred to as the submandibular route). The study used male CD1 and C57BL/6 strains of...

Year Published: 2023Topics: Biological Sampling & Physiological MeasurementAnimal Type: Mouse, Rodent

Citation: George, A. J., Harmsen, B. J., Ford, J. A. et al. 2023. Evaluation of submental blood collection in mice (Mus musculus). JAALAS 62(1), 92–98.

Non-invasive, real-time stress measurement: Vocalization compared with thermal imaging in kittens of the domestic cat in response to social separation

Finding tools to assess the stress response which can be easily applied, are non-invasive, reliable and measured in real time is still a relevant topic in many areas of biology. Vocal characteristics and temperature of certain body areas have been...

Year Published: 2023Topics: Biological Sampling & Physiological MeasurementAnimal Type: Cat

Citation: Szenczi, P., Jiménez Gallardo, A., Urrutia, A. et al. 2023. Non-invasive, real-time stress measurement: Vocalization compared with thermal imaging in kittens of the domestic cat in response to social separation. Behavioural Processes 213, 104955.

Measuring emotions in dairy cows: Relationships between infrared temperature of key body parts, lateralised behaviour and milk production

The emotions of cows could be reflected in their body temperature, productivity and behaviours. We tested whether there is a positive correlation between non-invasive infrared temperature (IRT) and right-side laterality (presumed to indicate anxiety), and negative correlation with milk production...

Year Published: 2023Topics: Biological Sampling & Physiological Measurement, Emotion, Pain, & SentienceAnimal Type: Cattle

Citation: Uddin, J., McNeill, D. M., Phillips, C. J. C. 2023. Measuring emotions in dairy cows: Relationships between infrared temperature of key body parts, lateralised behaviour and milk production. Applied Animal Behaviour Science 269, 106115.

Body surface temperature of rats reveals both magnitude and sex differences in the acute stress response

Understanding how biological markers of stress relate to stressor magnitude is much needed and can be used in welfare assessment. Changes in body surface temperature can be measured using infrared thermography (IRT) as a marker of a physiological response to...

Year Published: 2023Topics: Biological Sampling & Physiological Measurement, Welfare AssessmentAnimal Type: Rat, Rodent

Citation: Wongsaengchan, C., McCafferty, D. J., Evans, N. P. et al. 2023. Body surface temperature of rats reveals both magnitude and sex differences in the acute stress response. Physiology & Behavior 264, 114138.

Less invasive, simultaneous, and continuous measurements of locomotor activity and body temperature using the nano tag® small accelerometer device in cynomolgus monkeys

Locomotor activity and body temperature evaluations of cynomolgus monkeys are useful to understand the effects of drugs on the central nervous system. Here, we describe a simple, inexpensive, and less invasive evaluation method using the nano tag® (KISSEI COMTEC Co.,...

Year Published: 2022Topics: Biological Sampling & Physiological MeasurementAnimal Type: Macaque, Nonhuman Primate

Citation: Kishida, T., Motokawa, Y., Yokoi, R. et al. 2022. Less invasive, simultaneous, and continuous measurements of locomotor activity and body temperature using the nano tag® small accelerometer device in cynomolgus monkeys. Journal of Pharmacological and Toxicological Methods 118, 107224.

Monitoring blinks and eyelid twitches in horses to assess stress during the samples collection process

To assess stress in horses, a method of monitoring eye blinks (full and half) and eyelid twitches while collecting saliva and blood samples before and after annual intensive police training was carried out. This noninvasive monitoring method was used to...

Year Published: 2023Topics: Biological Sampling & Physiological MeasurementAnimal Type: Equine

Citation: Lelláková, M., Pavľak, A., Lešková, L. et al. 2023. Monitoring blinks and eyelid twitches in horses to assess stress during the samples collection process. Journal of Applied Animal Welfare Science 26(4), 530–539.

Implementation of an alternative training method for cardiac blood collection in mice

Training personnel to work with animals presents a variety of challenges, both logistically and with regard to animal welfare. These issues make training an ideal opportunity to evaluate practices and to implement the 3R principles (refinement, replacement, and reduction). Cardiac...

Year Published: 2023Topics: Animal Training, Biological Sampling & Physiological MeasurementAnimal Type: Mouse, Rodent

Citation: George, A. J., O'Malley, C. I., Bulock, R. E. et al. 2023. Implementation of an alternative training method for cardiac blood collection in mice. JAALAS 62(6), 487–493.