Biological Sampling & Physiological Measurement

Measuring emotions in dairy cows: Relationships between infrared temperature of key body parts, lateralised behaviour and milk production

The emotions of cows could be reflected in their body temperature, productivity and behaviours. We tested whether there is a positive correlation between non-invasive infrared temperature (IRT) and right-side laterality (presumed to indicate anxiety), and negative correlation with milk production...

Year Published: 2023Topics: Biological Sampling & Physiological Measurement, Emotion, Pain, & SentienceAnimal Type: Cattle

Citation: Uddin, J., McNeill, D. M., Phillips, C. J. C. 2023. Measuring emotions in dairy cows: Relationships between infrared temperature of key body parts, lateralised behaviour and milk production. Applied Animal Behaviour Science 269, 106115.

Body surface temperature of rats reveals both magnitude and sex differences in the acute stress response

Understanding how biological markers of stress relate to stressor magnitude is much needed and can be used in welfare assessment. Changes in body surface temperature can be measured using infrared thermography (IRT) as a marker of a physiological response to...

Year Published: 2023Topics: Biological Sampling & Physiological Measurement, Welfare AssessmentAnimal Type: Rat, Rodent

Citation: Wongsaengchan, C., McCafferty, D. J., Evans, N. P. et al. 2023. Body surface temperature of rats reveals both magnitude and sex differences in the acute stress response. Physiology & Behavior 264, 114138.

Less invasive, simultaneous, and continuous measurements of locomotor activity and body temperature using the nano tag® small accelerometer device in cynomolgus monkeys

Locomotor activity and body temperature evaluations of cynomolgus monkeys are useful to understand the effects of drugs on the central nervous system. Here, we describe a simple, inexpensive, and less invasive evaluation method using the nano tag® (KISSEI COMTEC Co.,...

Year Published: 2022Topics: Biological Sampling & Physiological MeasurementAnimal Type: Macaque, Nonhuman Primate

Citation: Kishida, T., Motokawa, Y., Yokoi, R. et al. 2022. Less invasive, simultaneous, and continuous measurements of locomotor activity and body temperature using the nano tag® small accelerometer device in cynomolgus monkeys. Journal of Pharmacological and Toxicological Methods 118, 107224.

Monitoring blinks and eyelid twitches in horses to assess stress during the samples collection process

To assess stress in horses, a method of monitoring eye blinks (full and half) and eyelid twitches while collecting saliva and blood samples before and after annual intensive police training was carried out. This noninvasive monitoring method was used to...

Year Published: 2023Topics: Biological Sampling & Physiological MeasurementAnimal Type: Equine

Citation: Lelláková, M., Pavľak, A., Lešková, L. et al. 2023. Monitoring blinks and eyelid twitches in horses to assess stress during the samples collection process. Journal of Applied Animal Welfare Science 26(4), 530–539.

Implementation of an alternative training method for cardiac blood collection in mice

Training personnel to work with animals presents a variety of challenges, both logistically and with regard to animal welfare. These issues make training an ideal opportunity to evaluate practices and to implement the 3R principles (refinement, replacement, and reduction). Cardiac...

Year Published: 2023Topics: Animal Training, Biological Sampling & Physiological MeasurementAnimal Type: Mouse, Rodent

Citation: George, A. J., O'Malley, C. I., Bulock, R. E. et al. 2023. Implementation of an alternative training method for cardiac blood collection in mice. JAALAS 62(6), 487–493.

Hair corticosterone concentration as a potential stress marker in pet rabbits

Background As a prey species, rabbits tend to hide their illnesses and injuries. Consequently, pet rabbit owners often do not notice that their pet may be suffering. Methods Data on the housing and health of 34 dwarf rabbits were collected...

Year Published: 2023Topics: Biological Sampling & Physiological MeasurementAnimal Type: Rabbit

Citation: Hube, D., van Meegen, C., Herbrandt, S. et al. 2023. Hair corticosterone concentration as a potential stress marker in pet rabbits. Veterinary Record 193(9), e3464.

A minimally invasive procedure for blood extraction from Xenopus laevis allows follow up studies without euthanasia

Blood extraction is extremely important for the development of scientific research; however, the existing methods for amphibian´s blood sampling are invasive, mainly leading to the euthanasia of the animal. Therefore, less intrusive methods that allow the obtention of multiple samples...

Year Published: 2024Topics: Biological Sampling & Physiological MeasurementAnimal Type: Amphibian, Frog & Toad

Citation: Jessica, C.-G., Buendía-González, L., Ruiz-Gómez, M. L. et al. 2024. A minimally invasive procedure for blood extraction from Xenopus laevis allows follow up studies without euthanasia. Journal of Applied Animal Welfare Science 27(1), 192–199.

Blood microsampling in cynomolgus monkey and evaluation of plasma PK parameters in comparison to conventional sampling

Microsampling, a reduced volume sampling method, has successfully gained attention at the International Conference on Harmonization (ICH) level and established benefits support its use in Toxicokinetic (TK) studies. These improved sampling techniques are less invasive and in large animal species...

Year Published: 2023Topics: Biological Sampling & Physiological MeasurementAnimal Type: Macaque, Nonhuman Primate

Citation: Bertani, S., Donadi, A., Franchi, J. et al. 2023. Blood microsampling in cynomolgus monkey and evaluation of plasma PK parameters in comparison to conventional sampling. Journal of Pharmacological and Toxicological Methods 123, 107298.

Aced it! Performing large volume survival bleeds in rabbits

New Zealand White rabbits are commonly used for polyclonal antibody production, which can require collecting no more than 10% of total body weight every two weeks (approximately 20 ml of blood for a 4 kg rabbit). In order to collect...

Year Published: 2023Topics: Biological Sampling & Physiological MeasurementAnimal Type: Rabbit

Citation: Brown, A. 2023. Aced it! Performing large volume survival bleeds in rabbits. Laboratory Animal Science Professional 11(3) (May/June), 44-46.

Using a cost-effective, efficient method to train young dogs to cooperate for multiple blood collection procedures in a pharmacokinetics study

Behavioral testing slings, such as the Pavlov sling, offer an effective form of restraint for beagles while minimizing stress. Although these slings provide effective restraint, their high cost makes them less likely to be considered when discussing restraint options, especially...

Year Published: 2023Topics: Animal Training, Biological Sampling & Physiological Measurement, RestraintAnimal Type: Dog

Citation: Rexroat, E. K., Haughton, C. L., Fox, K. 2023. Using a cost-effective, efficient method to train young dogs to cooperate for multiple blood collection procedures in a pharmacokinetics study. Laboratory Animal Science Professional 11(6) (November/December), 34-36.