Biological Sampling & Physiological Measurement

Can non-invasive glucocorticoid measures be used as reliable indicators of stress in animals?

Refinement techniques are being increasingly employed in all fields of animal research to try to ensure that the highest standards of welfare are upheld. This review concerns one of the main emerging techniques for the assessment of welfare itself, namely...

Year Published: 2006Topics: Biological Sampling & Physiological MeasurementAnimal Type: All/General

Citation: Lane, J. 2006. Can non-invasive glucocorticoid measures be used as reliable indicators of stress in animals? Animal Welfare 15(4), 331-342.

Issues to consider for preparing ferrets as research subjects in the laboratory

Successful housing requires knowledge of ferret behaviors including social behavior, eating habits, a general inquisitive nature, and a species-typical need to burrow and hide. Regular handling is necessary to maintain well-being. A ferret health care program consists of physical examination,...

Year Published: 2006Topics: Anesthesia & Sedation, Biological Sampling & Physiological Measurement, Euthanasia, Handling, Husbandry & Management, Natural Behavior, Rearing & Weaning, Relocation & Transport, Reproduction, RestraintAnimal Type: Ferret

Citation: Ball, R. S. 2006. Issues to consider for preparing ferrets as research subjects in the laboratory. ILAR Journal 47(4), 348-357.

Chapter 7.2. Injection and Blood Collection? How to minimize Stress Reactions

If the animals are under stress while you are working with them, there is a great risk that they show aggressive reactions to you, in an attempt to get away from the stressful situation. One of the conditions of successful...

Year Published: 2007Topics: Animal Training, Biological Sampling & Physiological MeasurementAnimal Type: All/General

Citation: LAREF [Laboratory Animal Refinement and Enrichment Forum] 2007. Chapter 7.2. Injection and Blood Collection?How to minimize Stress Reactions. In: Making Lives Easier for Animals in Research Labs: Discussions by the Laboratory Animal Refinement & Enrichment Forum. Baumans, V., Coke, C., Green, J., Moreau, E., Morton, D., Patterson-Kane, E., Reinhardt, A., Reinhardt, V., Van Loo, P. (eds), 117-124. Animal Welfare Institute, Washington, DC.

Applied behavior”” panel discussion

Adriane and Kris just trained eight rhesus monkeys to present their arms in the blood sleeve for blood samples, and it took, on average ... 8 months ... 160 minutes spread out into 40 sessions.

Year Published: 2007Topics: Animal Training, Biological Sampling & Physiological MeasurementAnimal Type: Macaque, Nonhuman Primate

Citation: Schapiro, S. J., Laule, G., Seelig, D. 2007. "Applied behavior" panel discussion. Journal of Applied Animal Welfare Science 10(1), 79-81.

Effectiveness of saliva collection and enzyme-immunoassay for the quantification of cortisol in socially housed baboons

Circulating cortisol levels are often used to assess the biological stress response in captive primates. Some methods commonly used to collect blood samples may alter the stress response. As such, noninvasive means to analyze cortisol levels are increasingly being developed....

Year Published: 2008Topics: Biological Sampling & Physiological MeasurementAnimal Type: Baboon, Nonhuman Primate

Citation: Pearson, B. L., Judge, P., Reeder, D. M. 2008. Effectiveness of saliva collection and enzyme-immunoassay for the quantification of cortisol in socially housed baboons. American Journal of Primatology 70(12), 1145-1151.

Quality of plasma sampled by different methods for multiple blood sampling in mice

For oral glucose tolerance test (OGTT) in mice, multiple blood samples need to be taken within a few hours from conscious mice. Today, a number of essential parameters may be analysed on very small amounts of plasma, thus reducing the...

Year Published: 2009Topics: Biological Sampling & Physiological MeasurementAnimal Type: Mouse, Rodent

Citation: Christensen, S. D., Mikkelsen, L. F., Fels, J. J. et al. 2009. Quality of plasma sampled by different methods for multiple blood sampling in mice. Laboratory Animals 43, 65-71.

A jugular bleeding technique in rabbits

When studying pharmacokinetics in rabbits, researchers must often take multiple blood samples from conscious rabbits. Researchers usually collect these samples via the auricular vein, typically through a port or an indwelling catheter. The authors have developed an easy and efficient...

Year Published: 2010Topics: Biological Sampling & Physiological Measurement, Cannulation, Catheterization, & IntubationAnimal Type: Rabbit

Citation: Nelson, E. A., Keller, G. L., Mitchell, T. W. et al. 2010. A jugular bleeding technique in rabbits. Lab Animal 39(1), 17-22.

Clinical biochemistry parameters in C57BL/6J mice after blood collection from the submandibular vein and retroorbital plexus

Collection of blood from the submandibular vein allows simple and rapid processing of many animals without anesthesia and facilitates rapid recovery with no signs of pain and discomfort in the mice. Here we compared the submandibular vein and retroorbital plexus...

Year Published: 2010Topics: Biological Sampling & Physiological MeasurementAnimal Type: Mouse, Rodent

Citation: Fernandez, I., Pena, A., Del Teso, N. et al. 2010. Clinical biochemistry parameters in C57BL/6J mice after blood collection from the submandibular vein and retroorbital plexus. JAALAS 49(2), 202-206.

Quality of blood samples from the saphenous vein compared with the tail vein during multiple blood sampling of mice

A large number of methods for obtaining blood from mice have been published. In our facility, saphenous venepuncture is considered the method of choice for bleeding mice and is believed to have a number of welfare advantages when performed correctly....

Year Published: 2010Topics: Biological Sampling & Physiological MeasurementAnimal Type: Mouse, Rodent

Citation: Aasland, K. E., Skjerve, E., Smith, A. J. 2010. Quality of blood samples from the saphenous vein compared with the tail vein during multiple blood sampling of mice. Laboratory Animals 44(1), 25-29.

Comparative analysis of blood sampling techniques in the rat

Blood sampling is one of the most common procedures performed on laboratory animals as part of scientific research. As an ongoing commitment, our institution has identified a need to improve the current blood sampling method in rats from both a...

Year Published: 2011Topics: Biological Sampling & Physiological MeasurementAnimal Type: Rat, Rodent

Citation: Mathieu, C. 2011. Comparative analysis of blood sampling techniques in the rat. American Association for Laboratory Animal Science [AALAS] Meeting Official Program, 738 (Abstract #PS81).