Zebrafish

Behavioral and neurophysiological effects of electrical stunning on zebrafish larvae

Two methods dominate the way that zebrafish larvae are euthanized after experimental procedures: anesthetic overdose and rapid cooling. Although MS-222 is easy to apply, this anesthetic takes about a minute to act and fish show aversive reactions and interindividual differences,...

Year Published: 2025Topics: EuthanasiaAnimal Type: Fish, Zebrafish

Citation: Burkhardt, D.-S., Leyden, C., Thomas, C. et al. 2025. Behavioral and neurophysiological effects of electrical stunning on zebrafish larvae. Lab Animal 54(2), 50–58.

Good welfare is attractive: Female zebrafish (Danio rerio) prefer males from complex, well-resourced conditions over males from conventional barren laboratory tanks

Applied ethologists often find that sub-optimal housing (e.g. barren versus “enriched”, well-resourced conditions) impairs animals’ interactions with conspecifics. Furthermore, some housing effects on social/sexual interactions persist even in standardised test situations. For example, in mating tests on three mammalian and...

Year Published: 2025Topics: Environmental Enrichment, ReproductionAnimal Type: Fish, Zebrafish

Citation: Lavery, J. M., Snaith, K., Pallarca, J. et al. 2025. Good welfare is attractive: Female zebrafish (Danio rerio) prefer males from complex, well-resourced conditions over males from conventional barren laboratory tanks. Applied Animal Behaviour Science 286, 106603.

Novel euthanasia technique for zebrafish using electric shock in standard group housing aquaria

Zebrafish are widely used in research, accounting for approximately 50% of all laboratory fish across Europe. Due to their rapid reproduction and aging, effective and practical euthanasia methods are necessary for managing large stocks. This study investigated whether adult zebrafish...

Year Published: 2025Topics: EuthanasiaAnimal Type: Fish, Zebrafish

Citation: Saarinen, U., Sundell, E., Sneddon, L. et al. 2025. Novel euthanasia technique for zebrafish using electric shock in standard group housing aquaria. Scientific Reports 15(1), 3011.

Tricaine, eugenol and etomidate for repetitive procedural anesthesia in adult zebrafish, Danio rerio: Effect on stress and behavior (2025)

Zebrafish has emerged as a popular animal model in biomedical research. Numerous procedures and interventions require occasionally repetitive anesthesia. Tricaine is the most frequently used anesthetic for zebrafish and its efficacy is well established. However, the safety and efficacy of...

Year Published: 2025Topics: Anesthesia & SedationAnimal Type: Fish, Zebrafish

Citation: Narumanchi, S., Perttunen, S., Laine, P. et al. 2025. Tricaine, eugenol and etomidate for repetitive procedural anesthesia in adult zebrafish, Danio rerio: Effect on stress and behavior. Frontiers in Veterinary Science 12.

VMS-Fish: A comprehensive application for zebrafish husbandry management (2025)

Zebrafish are widely used and increasingly adopted in developmental biology, disease modeling and tissue regeneration research. However, a freely available and user-friendly zebrafish husbandry management application has been lacking. Here, we developed an open-source fish management application, referred to as...

Year Published: 2025Topics: Husbandry & ManagementAnimal Type: Fish, Zebrafish

Citation: Muraleedharan Saraswathy, V., Mokalled, M. H. 2025. VMS-Fish: A comprehensive application for zebrafish husbandry management. Zebrafish 22(2), 59–63.

Evaluation of tricaine methanesulfonate concentrations for flow anesthesia in zebrafish (Danio rerio) (2025)

Zebrafish (Danio rerio) are often anesthetized by immersion in buffered tricaine methanesulfonate (MS-222). Although commonly utilized, immersion anesthesia presents a shortcoming of lethal asphyxiation with increased duration of exposures. A newer technique that circumvents this issue, known as flow anesthesia,...

Year Published: 2025Topics: Anesthesia & SedationAnimal Type: Fish, Zebrafish

Citation: Kramer, A. G., Bern, L. R., Ferguson, L. T. et al. 2025. Evaluation of tricaine methanesulfonate concentrations for flow anesthesia in zebrafish (Danio rerio). Zebrafish 22(3), 99–106.

Advancing zebrafish husbandry: takeaways from the 2024 husbandry workshop and husbandry summit (2024)

The 2024 Zebrafish Husbandry Workshop and Summit held during the World Aquaculture Society Meeting in San Antonio focused on key areas for improving zebrafish husbandry research. Discussions highlighted the need for comprehensive literature on husbandry, better communication and collaboration between...

Year Published: 2024Topics: Housing, Husbandry & ManagementAnimal Type: Fish, Zebrafish

Citation: Altemara, M., Baur, B., Varga, Z. M. 2024. Advancing Zebrafish Husbandry: Takeaways From the 2024 Husbandry Workshop and Husbandry Summit. Zebrafish 21(6), 418–422.

Novel euthanasia technique for zebrafish using electric shock in standard group housing aquaria

Year Published: 2025Topics: EuthanasiaAnimal Type: Fish, Zebrafish

Citation: Saarinen, U., Sundell, E., Sneddon, L. et al. 2025. Novel euthanasia technique for zebrafish using electric shock in standard group housing aquaria. Scientific Reports 15(1), 3011.

Zebrafish maintenance conditions affect behavioral and biochemical biomarkers: A possible interfering factor on the research results

Over the years, scientific research with fish models has grown at a rapid pace, and issues such as animal welfare are becoming increasingly important in various areas of animal husbandry and experimentation. Here, we evaluated whether Danio rerio behavior is...

Year Published: 2024Topics: Environmental EnrichmentAnimal Type: Fish, Zebrafish

Citation: Amaral, F. U. I., Pompermaier, A., Almeida, P. A. de et al. 2024. Zebrafish maintenance conditions affect behavioral and biochemical biomarkers: A possible interfering factor on the research results. Zebrafish.

Effects of housing density on anxiety-like behavior of zebrafish in the plus maze with ramp

Population density in experimental animals is a crucial factor in maintaining the wellbeing of the organisms. Inadequate housing conditions can compromise the validity and reliability of research results, making comparisons between studies difficult. In sociable species such as zebrafish (Danio...

Year Published: 2024Topics: Stocking DensityAnimal Type: Fish, Zebrafish

Citation: Pessoa, R., Motta, C., Araujo-Pessoa, E. et al. 2024. Effects of housing density on anxiety-like behavior of zebrafish in the plus maze with ramp. Behavioural Processes 222, 105114.