Mouse

A multicentre study on spontaneous in-cage activity and micro-environmental conditions of IVC housed C57BL/6J mice during consecutive cycles of bi-weekly cage-change

Mice respond to a cage change (CC) with altered activity, disrupted sleep and increased anxiety. A bi-weekly cage change is, therefore, preferred over a shorter CC interval and is currently the prevailing routine for Individually ventilated cages (IVCs). However, the...

Year Published: 2022Topics: Husbandry & ManagementAnimal Type: Mouse, Rodent

Citation: Ulfhake, B., Lerat, H., Honetschlager, J. et al. 2022. A multicentre study on spontaneous in-cage activity and micro-environmental conditions of IVC housed C57BL/6J mice during consecutive cycles of bi-weekly cage-change. PLOS ONE 17(5), e0267281.

Pivotal role of micro-CT technology in setting up an optimized lung fibrosis mouse model for drug screening

Idiopathic pulmonary fibrosis (IPF) is a progressive disease with no curative pharmacological treatment. The most used animal model of IPF for anti-fibrotic drug screening is bleomycin (BLM)-induced lung fibrosis. However, several issues have been reported: the balance among disease resolution,...

Year Published: 2022Topics: Disease/Experimental ModelAnimal Type: Mouse, Rodent

Citation: Khalajzeyqami, Z., Grandi, A., Ferrini, E. et al. 2022. Pivotal role of micro-CT technology in setting up an optimized lung fibrosis mouse model for drug screening. PLOS ONE 17(6), e0270005.

Waking inactivity as a welfare indicator in laboratory mice: Investigating postures, facial expressions and depression-like states

Animal welfare assessment relies on valid and practical indicators of affect. In mice, the most widely used research vertebrates, lying still with eyes open, inactive-but-awake (IBA) in the home cage, has potential to be one such indicator. IBA is elevated...

Year Published: 2022Topics: Emotion, Pain, & Sentience, Welfare AssessmentAnimal Type: Mouse, Rodent

Citation: MacLellan, A., Nazal, B., Young et al. 2022. Waking inactivity as a welfare indicator in laboratory mice: Investigating postures, facial expressions and depression-like states. Royal Society Open Science 9(11), 221083.

Effects of separated pair housing of female C57BL/6JRj mice on well-being

In laboratory animal facilities, it is a common code of practice to house female mice in groups. However, some experimental conditions require to house them individually, even though social isolation may impair their well-being. Therefore, we introduced a separated pair...

Year Published: 2022Topics: Social Housing & CompanionshipAnimal Type: Mouse, Rodent

Citation: Hohlbaum, K., Merle, R., Frahm, S. et al. 2022. Effects of separated pair housing of female C57BL/6JRj mice on well-being. Scientific Reports 12(1), 8819.

Noninvasive three-state sleep-wake staging in mice using electric field sensors

Study Objective: Validate a novel method for sleep-wake staging in mice using noninvasive electric field (EF) sensors. Methods: Mice were implanted with electroencephalogram (EEG) and electromyogram (EMG) electrodes and housed individually. Noninvasive EF sensors were attached to the exterior of...

Year Published: 2020Topics: Biological Sampling & Physiological MeasurementAnimal Type: Mouse, Rodent

Citation: Kloefkorn H, Aiani, L. M., Lakhani, A. et al. 2020. Noninvasive three-state sleep-wake staging in mice using electric field sensors. Journal of Neuroscience Methods 344, 108834.

Evaluation of active warming and surgical draping for perioperative thermal support in laboratory mice

Surgical procedures are commonly performed using mice but can have major effects on their core body temperature, including development of hypothermia. In this study, we evaluated active perioperative warming with and without surgical draping with adherent plastic wrap to refine...

Year Published: 2022Topics: Surgery & Post-OpAnimal Type: Mouse, Rodent

Citation: Bailey, K. T., Jantre, S. R., Lawrence, F. R. et al. 2022. Evaluation of active warming and surgical draping for perioperative thermal support in laboratory mice. JAALAS 61(5), 482-494.

Refinements to rodent head fixation and fluid/food control for neuroscience

The use of head fixation in mice is increasingly common in research, its use having initially been restricted to the field of sensory neuroscience. Head restraint has often been combined with fluid control, rather than food restriction, to motivate behaviour,...

Year Published: 2022Topics: Behavioral & Cognitive Testing, RestraintAnimal Type: Mouse, Rodent

Citation: Barkus, C., Bergmann, C., Branco, T. et al. 2022. Refinements to rodent head fixation and fluid/food control for neuroscience. Journal of Neuroscience Methods 381, 109705.

3R measures in facilities for the production of genetically modified rodents

Sociocultural changes in the human–animal relationship have led to increasing demands for animal welfare in biomedical research. The 3R concept is the basis for bringing this demand into practice: Replace animal experiments with alternatives where possible, Reduce the number of...

Year Published: 2022Topics: Analgesia, Disease/Experimental Model, Husbandry & Management, Reproduction, Surgery & Post-OpAnimal Type: Mouse, Rat, Rodent

Citation: Zevnik, B., Jerchow, B., Buch, T. 2022. 3R measures in facilities for the production of genetically modified rodents. Lab Animal 51(6), 162-177.

A quick and low-intensity method for oral administration to large numbers of mice: A possible alternative to oral gavage

Oral administration of medication to experimental animals is a cause of significant stress. When coupled to animals who are already under strenuous circumstances due to the disease being modelled, there is a significant risk for increased morbidity and mortality, thus...

Year Published: 2022Topics: Drug/Substance AdministrationAnimal Type: Mouse, Rodent

Citation: Neto, T., Faustino-Rocha, A. I., Gil da Costa, R. M. et al. 2022. A quick and low-intensity method for oral administration to large numbers of mice: A possible alternative to oral gavage. Laboratory Animals 56(2), 185-190.

Early-life mice housed in standard stocking density reduce the spontaneous physical activity and increase visceral fat deposition before reaching adulthood

Laboratory rodents spend the entire day housed in standard cages that provide a restricted area for movements and might, therefore, limit physical activity. However, it has not been tested in immature rodents of ages ranging from weaning to adulthood (adolescence...

Year Published: 2022Topics: Stocking DensityAnimal Type: Mouse, Rodent

Citation: Scariot, P. P., Gobatto, C. A., Polisel, E. E. et al. 2022. Early-life mice housed in standard stocking density reduce the spontaneous physical activity and increase visceral fat deposition before reaching adulthood. Laboratory Animals 56(4), 344-355.