Identification Method

Evaluation of healing progression at surgical incision sites and the use of antiseptics for enhancing post-operative survival in subyearling Chinook salmon (Oncorhynchus tshawytscha)

In an attempt to develop more effective surgical implantation methods for fish, surgical incisions typical of those made for implanting micro-acoustic transmitters into the peritoneal cavity were evaluated on a weekly basis for healing progression using a suite of metrics....

Year Published: 2023Topics: Identification Method, Surgery & Post-OpAnimal Type: Fish, Salmon

Citation: Cameron, A. S., Rub, A. M. W., Sandford, B. P. 2023. Evaluation of healing progression at surgical incision sites and the use of antiseptics for enhancing post-operative survival in subyearling Chinook salmon (Oncorhynchus tshawytscha). PLOS ONE 18(7), e0288056.

Image-based automatic individual identification of fish without obvious patterns on the body (scale pattern)

The precision fish farming concept has been widely investigated in research and is highly desirable in aquaculture as it creates opportunities for precisely controlling and monitoring fish cultivation processes and increasing fish welfare. The automatic identification of individual fish could...

Year Published: 2022Topics: Identification MethodAnimal Type: Fish, Other Fish

Citation: Bekkozhayeva, D., Cisar, P. 2022. Image-based automatic individual identification of fish without obvious patterns on the body (scale pattern). Applied Sciences 12(11), 5401.

Tetra-primer amplification-refractory mutationsystem (ARMS)—PCR for genotyping mouse leptin gene mutation

Due to spontaneous deficiency in leptin, ob/ob mice are one of the most commonly used experimental animal models in diabetes research. In this study, we reported a quick and easy-to-conduct genotyping method using tetra-primer amplification refractory mutation system-polymerase chain reaction...

Year Published: 2022Topics: Disease/Experimental Model, Identification MethodAnimal Type: Mouse, Rodent

Citation: Chen, J., Xu, X., Dalhaimer, P. et al. 2022. Tetra-primer amplification-refractory mutationsystem (ARMS)—PCR for genotyping mouse leptin gene mutation. Animals 12(19), 2680.

A rapid and noninvasive method that extracts polymerase chain reaction-ready genomic DNA from adult zebrafish

Genotyping usually entails analysis of the products of polymerase chain reaction (PCR) carried out with genomic DNA (gDNA) as template, and is employed for validation of mutant or transgenic organisms. For genotyping of adult zebrafish, gDNA is often extracted from...

Year Published: 2022Topics: Identification MethodAnimal Type: Fish, Zebrafish

Citation: Jang, J. Y., Liang, T., Kim, M.-K. et al. 2022. A rapid and noninvasive method that extracts polymerase chain reaction-ready genomic DNA from adult zebrafish. Zebrafish 19(4), 160-164.

Evaluation of six methods for external attachment of electronic tags to fish: Assessment of tag retention, growth and fish welfare

External attachment of electronic tags has been increasingly used in fish studies. Many researchers have used ad hoc attachment methods and provided little or no validation for the assumption that tagging itself does not bias animal behaviour or survival. The...

Year Published: 2022Topics: Identification MethodAnimal Type: Fish, Other Fish

Citation: Runde, B. J., Buckel, J. A., Bacheler, N. M. et al. 2022. Evaluation of six methods for external attachment of electronic tags to fish: Assessment of tag retention, growth and fish welfare. Journal of Fish Biology 101(3), 419-430.

The pen is milder than the blade: Identification marking mice using ink on the tail appears more humane than ear-punching even with local anaesthetic

Identification marking mice commonly involves ear-punching with or without anaesthetic, or tail-marking with ink. To identify which is most humane, we marked weanling male BALB/c mice using ear-punching (EP), ear-punching with anaesthetic EMLATM cream (EP+A), or permanent marker pen (MP)....

Year Published: 2021Topics: Identification MethodAnimal Type: Mouse, Rodent

Citation: Burn, C. C., Mazlan, N. H. B., Chancellor, N. et al. 2021. The pen is milder than the blade: Identification marking mice using ink on the tail appears more humane than ear-punching even with local anaesthetic. Animals 11(6), 1664.

Identification of individual zebrafish (Danio rerio): A refined protocol for VIE tagging whilst considering animal welfare and the principles of the 3Rs

In aquatic ecology, studies have commonly employed a tagging technique known as visible implant elastomer (VIE). This method has not been widely adopted by the zebrafish research community and also lacks refinement with regard to animal welfare. The current paper...

Year Published: 2021Topics: Identification MethodAnimal Type: Fish, Zebrafish

Citation: Rácz, A., Allan, B., Dwyer, T. et al. 2021. Identification of individual zebrafish (Danio rerio): A refined protocol for VIE tagging whilst considering animal welfare and the principles of the 3Rs. Animals 11(3), 616.

A minimally invasive technique to assess several life-history characteristics of the endangered great hammerhead shark Sphyrna mokarran

A dorsal-fin photo-identification technique paired with a non-invasive parallel laser photogrammetry technique was used to non-invasively identify individual Sphyrna mokarran over time. Based on the data collected over a duration of 59 days, 16 different S. mokarran (mean ± s.d....

Year Published: 2016Topics: Identification MethodAnimal Type: Amphibian, Frog & Toad

Citation: O'Connell, C. P., Leurs, G. 2016. A minimally invasive technique to assess several life-history characteristics of the endangered great hammerhead shark Sphyrna mokarran. Journal of Fish Biology 88(3), 1257-1264.

International survey on the use and welfare of zebrafish Danio rerio in research

A survey was conducted regarding zebrafish Danio rerio use for scientific research with a focus on: anaesthesia and euthanasia; housing and husbandry; breeding and production; refinement opportunities. A total of 98 survey responses were received from laboratories in 22 countries...

Year Published: 2017Topics: Anesthesia & Sedation, Environmental Enrichment, Euthanasia, Housing, Husbandry & Management, Identification Method, ReproductionAnimal Type: Fish, Zebrafish

Citation: Lidster, K., Readman, G. D., Prescott, M. J. et al. 2017. International survey on the use and welfare of zebrafish Danio rerio in research. Journal of Fish Biology 90(5), 1891-1905.

Welfare and scientific considerations of tattooing and ear tagging for mouse identification

Ear tagging is perceived as less painful or stressful than tattooing and therefore is generally considered less harmful or costly to welfare. However, ear tags are more difficult to read than tattoos and can fall out, and mice usually require...

Year Published: 2019Topics: Identification MethodAnimal Type: Mouse, Rodent

Citation: Roughan, J. V., Sevenoaks, T. 2019. Welfare and scientific considerations of tattooing and ear tagging for mouse identification. JAALAS 58(2), 142-153.