Biological Sampling & Physiological Measurement

Effectiveness of saliva collection and enzyme-immunoassay for the quantification of cortisol in socially housed baboons

Circulating cortisol levels are often used to assess the biological stress response in captive primates. Some methods commonly used to collect blood samples may alter the stress response. As such, noninvasive means to analyze cortisol levels are increasingly being developed....

Year Published: 2008Topics: Biological Sampling & Physiological MeasurementAnimal Type: Baboon, Nonhuman Primate

Citation: Pearson, B. L., Judge, P., Reeder, D. M. 2008. Effectiveness of saliva collection and enzyme-immunoassay for the quantification of cortisol in socially housed baboons. American Journal of Primatology 70(12), 1145-1151.

Quality of plasma sampled by different methods for multiple blood sampling in mice

For oral glucose tolerance test (OGTT) in mice, multiple blood samples need to be taken within a few hours from conscious mice. Today, a number of essential parameters may be analysed on very small amounts of plasma, thus reducing the...

Year Published: 2009Topics: Biological Sampling & Physiological MeasurementAnimal Type: Mouse, Rodent

Citation: Christensen, S. D., Mikkelsen, L. F., Fels, J. J. et al. 2009. Quality of plasma sampled by different methods for multiple blood sampling in mice. Laboratory Animals 43, 65-71.

A jugular bleeding technique in rabbits

When studying pharmacokinetics in rabbits, researchers must often take multiple blood samples from conscious rabbits. Researchers usually collect these samples via the auricular vein, typically through a port or an indwelling catheter. The authors have developed an easy and efficient...

Year Published: 2010Topics: Biological Sampling & Physiological Measurement, Cannulation, Catheterization, & IntubationAnimal Type: Rabbit

Citation: Nelson, E. A., Keller, G. L., Mitchell, T. W. et al. 2010. A jugular bleeding technique in rabbits. Lab Animal 39(1), 17-22.

Clinical biochemistry parameters in C57BL/6J mice after blood collection from the submandibular vein and retroorbital plexus

Collection of blood from the submandibular vein allows simple and rapid processing of many animals without anesthesia and facilitates rapid recovery with no signs of pain and discomfort in the mice. Here we compared the submandibular vein and retroorbital plexus...

Year Published: 2010Topics: Biological Sampling & Physiological MeasurementAnimal Type: Mouse, Rodent

Citation: Fernandez, I., Pena, A., Del Teso, N. et al. 2010. Clinical biochemistry parameters in C57BL/6J mice after blood collection from the submandibular vein and retroorbital plexus. JAALAS 49(2), 202-206.

Quality of blood samples from the saphenous vein compared with the tail vein during multiple blood sampling of mice

A large number of methods for obtaining blood from mice have been published. In our facility, saphenous venepuncture is considered the method of choice for bleeding mice and is believed to have a number of welfare advantages when performed correctly....

Year Published: 2010Topics: Biological Sampling & Physiological MeasurementAnimal Type: Mouse, Rodent

Citation: Aasland, K. E., Skjerve, E., Smith, A. J. 2010. Quality of blood samples from the saphenous vein compared with the tail vein during multiple blood sampling of mice. Laboratory Animals 44(1), 25-29.

Comparative analysis of blood sampling techniques in the rat

Blood sampling is one of the most common procedures performed on laboratory animals as part of scientific research. As an ongoing commitment, our institution has identified a need to improve the current blood sampling method in rats from both a...

Year Published: 2011Topics: Biological Sampling & Physiological MeasurementAnimal Type: Rat, Rodent

Citation: Mathieu, C. 2011. Comparative analysis of blood sampling techniques in the rat. American Association for Laboratory Animal Science [AALAS] Meeting Official Program, 738 (Abstract #PS81).

Glucocorticoid metabolites in rabbit faeces – Influence of environmental enrichment and cage size

The concentration of glucocorticoid metabolites (GCM) in rabbit faeces has been suggested as a non-invasive indicator of stress. In the present study, GCM concentrations were measured in faeces of fattening rabbits kept in groups of eight, at seven different stocking...

Year Published: 2011Topics: Biological Sampling & Physiological Measurement, Environmental EnrichmentAnimal Type: Rabbit

Citation: Buijs, S., Maertens, L., Tuyttens, F. A. M. et al. 2011. Glucocorticoid metabolites in rabbit faeces - Influence of environmental enrichment and cage size. Physiology & Behavior 104(3), 469-473.

The choice of bedding substrate, route of blood collection, and method of glucose determination all affect fasting blood glucose levels in C57BL/6 mice

This study compared 4 bedding substrates (corncob, paper, hardwood chip, and wire-bottomed cage inserts) and 2 routes of blood collection (lateral tail vein and retroorbital sinus) in 6 male C57BL/6NCrl mice. Mice were maintained with ad libitum food and water...

Year Published: 2011Topics: Biological Sampling & Physiological Measurement, Husbandry & ManagementAnimal Type: Mouse, Rodent

Citation: Zahorsky-Reeves, J. 2011. The choice of bedding substrate, route of blood collection, and method of glucose determination all affect fasting blood glucose levels in C57BL/6 mice. American Association for Laboratory Animal Science [AALAS] Meeting Official Program, 738 (Abstract #PS80).

Training rhesus macaques for conscious blood sampling in six steps

Step 1 Gently squeeze the animal about halfway towards you. Hold the animal in place for about 1 to 2 seconds.We use this time to talk to the animal calmly in a gentle voice. When the time is up, release...

Year Published: 2011Topics: Animal Training, Biological Sampling & Physiological MeasurementAnimal Type: Macaque, Nonhuman Primate

Citation: Weissman-Socci, S., Ray, K. D. 2011. Training rhesus macaques for conscious blood sampling in six steps. Tech Talk [The Newsletter for Laboratory Animal Science Technicians] 16(6), 3.

Training time required to collect a variety of biological samples using primarily positive reinforcement training methods

Positive reinforcement training (PRT) methods can be applied to gain the voluntary cooperation of laboratory primates with the collection of biological samples. Information will be provided on training techniques used and the amount of training time required for various sample...

Year Published: 2013Topics: Animal Training, Biological Sampling & Physiological MeasurementAnimal Type: Chimpanzee, Macaque, Nonhuman Primate

Citation: Bloomsmith, M., Franklin, A., Neu, K. et al. 2013. Training time required to collect a variety of biological samples using primarily positive reinforcement training methods. American Journal of Primatology 75(S1), 65. (36th Meeting of the American Society of Primatologists Scientific Program, Abstract #112)