Biological Sampling & Physiological Measurement

Comparison of serial blood collection by facial vein and retrobulbar methods in C57BL/6 mice

Many biomedical research protocols for mouse models involve serial blood collection and analysis. Two common techniques for serial blood collection in this species are the retrobulbar (RB, also called retroorbital) and facial vein (FV) methods. However, previous studies comparing these...

Year Published: 2018Topics: Biological Sampling & Physiological MeasurementAnimal Type: Mouse, Rodent

Citation: Frohlich, J. R., Alarcón, C. N., Toarmino, C. R. et al. 2018. Comparison of serial blood collection by facial vein and retrobulbar methods in C57BL/6 mice. JAALAS 57(4), 382-391.

The effect of early life experience, environment, and genetic factors on spontaneous home-cage aggression-related wounding in male C57BL/6 mice

Aggression is a major welfare issue in mice, particularly when mice unfamiliar to each other are first placed in cages, as happens on receipt from a vendor, and following cage cleaning. Injuries from aggression are the second leading cause of...

Year Published: 2017Topics: Biological Sampling & Physiological Measurement, Husbandry & Management, Social Housing & CompanionshipAnimal Type: Mouse, Rodent

Citation: Gaskill, B. N., Stottler, A. M., Garner, J. P. et al. 2017. The effect of early life experience, environment, and genetic factors on spontaneous home-cage aggression-related wounding in male C57BL/6 mice. Lab Animal 46(4), 176-184.

A rat-friendly modification of the non-invasive tail-cuff to record blood pressure

In animal models, blood pressure measurement methods can be either invasive (direct) or non-invasive (indirect). The non-invasive alternative involves applying a tail-cuff for blood pressure measurement. Current standardized restraint methods involve confining the laboratory animal in plastic chambers, which can...

Year Published: 2017Topics: Biological Sampling & Physiological Measurement, RestraintAnimal Type: Rat, Rodent

Citation: Lipták, B., Kaprinay, B., Gáspárová, Z. 2017. A rat-friendly modification of the non-invasive tail-cuff to record blood pressure. Lab Animal 46(6), 251-253.

Refinement of a model of repeated cerebrospinal fluid collection in conscious rats

The cannulation of the cisterna magna in rats for in vivo sampling of cerebrospinal fluid serves as a valuable model for studying the delivery of new drugs into the central nervous system or disease models. It offers the advantages of...

Year Published: 2017Topics: Biological Sampling & Physiological MeasurementAnimal Type: Rat, Rodent

Citation: Amen, E. M., Brecheisen, M., Sach-Peltason, L. et al. 2017. Refinement of a model of repeated cerebrospinal fluid collection in conscious rats. Laboratory Animals 51(1), 44-53.

A comparison of various methods of blood sampling in mice and rats: Effects on animal welfare

This study was conducted to investigate the effects of blood sampling on animal welfare in a total of 60 NTac:SD rats and 72 C57BL/6NTac mice of both sexes. Blood was sampled either by sublingual vein puncture, tail vein puncture or...

Year Published: 2018Topics: Biological Sampling & Physiological MeasurementAnimal Type: Mouse, Rat, Rodent

Citation: Harikrishnan, V. S., Hansen, A. K., Abelson, K. S. P. et al. 2018. A comparison of various methods of blood sampling in mice and rats: Effects on animal welfare. Laboratory Animals 52(3), 253-264.

The gingival vein as a minimally traumatic site for multiple blood sampling in guinea pigs and hamsters

Laboratory animals are still necessary in scientific investigation and vaccine testing, but while novel methodological approaches are not available for their replacement, the search for new, humane, easy, and painless methods is necessary to diminish their stress and pain. When...

Year Published: 2017Topics: Biological Sampling & Physiological MeasurementAnimal Type: Guinea Pig, Hamster, Rodent

Citation: Rodrigues, M. V., de Castro, S. O., de Albuquerque, C. Z. 2017. The gingival vein as a minimally traumatic site for multiple blood sampling in guinea pigs and hamsters. PLOS ONE 12(5), e0177967.

Laparoscopic technique for serial collection of para-colonic, left colic, and inferior mesenteric lymph nodes in macaques

Unlike peripheral lymph nodes (PLN), the mesenteric lymph nodes (MLN) draining the gastrointestinal (GI) tract are exposed to microbes and microbial products from the intestines and as such, are immunologically distinct. GI draining (MLN) have also been shown to be...

Year Published: 2016Topics: Biological Sampling & Physiological MeasurementAnimal Type: Macaque, Nonhuman Primate

Citation: Smedley, J., Macalister, R., Wangari, S. et al. 2016. Laparoscopic technique for serial collection of para-colonic, left colic, and inferior mesenteric lymph nodes in macaques. PLOS ONE 11(6), e0157535.

Measurements of core temperature in spontaneously hypertensive rats by radiotelemetry

Handling and restraint can cause significant increases in core temperature. When SH [spontaneously hypertensive] rats are not stressed or disturbed, their core temperatures are not significantly different from that of normotensive WKY [Wistar-Kyoto] rats.

Year Published: 1990Topics: Biological Sampling & Physiological MeasurementAnimal Type: Rat, Rodent

Citation: Berkey, D. L., Meeuwsen, K. W., Barney, C. C. 1990. Measurements of core temperature in spontaneously hypertensive rats by radiotelemetry. American Journal of Physiology 258, R743-749.

Cortisol response of female rhesus monkeys to venipuncture in homecage versus venipuncture in restraint apparatus

All subjects were well habituated to blood collection, and it was not necessary to immobilize them; they readily presented a leg for venipuncture. In single monkeys venipunctured in the restraint apparatus, cortisol concentrations were on average 50% higher 15 minutes...

Year Published: 1990Topics: Biological Sampling & Physiological Measurement, RestraintAnimal Type: Macaque, Nonhuman Primate

Citation: Reinhardt, V., Cowley, D., Scheffler, J. et al. 1990. Cortisol response of female rhesus monkeys to venipuncture in homecage versus venipuncture in restraint apparatus. Journal of Medical Primatology 19, 601-606.

New methodology for measuring blood pressure in awake baboons with use of behavioral training techniques

Training protocols are described to ensure cooperation of 10 single-housed baboons during blood pressure measurement in the homecage.

Year Published: 1990Topics: Animal Training, Biological Sampling & Physiological MeasurementAnimal Type: Baboon, Nonhuman Primate

Citation: Turkkan, J. S. 1990. New methodology for measuring blood pressure in awake baboons with use of behavioral training techniques. Journal of Medical Primatology 19, 455-466.