Biological Sampling & Physiological Measurement

Laparoscopic technique for serial collection of para-colonic, left colic, and inferior mesenteric lymph nodes in macaques

Unlike peripheral lymph nodes (PLN), the mesenteric lymph nodes (MLN) draining the gastrointestinal (GI) tract are exposed to microbes and microbial products from the intestines and as such, are immunologically distinct. GI draining (MLN) have also been shown to be...

Year Published: 2016Topics: Biological Sampling & Physiological MeasurementAnimal Type: Macaque, Nonhuman Primate

Citation: Smedley, J., Macalister, R., Wangari, S. et al. 2016. Laparoscopic technique for serial collection of para-colonic, left colic, and inferior mesenteric lymph nodes in macaques. PLOS ONE 11(6), e0157535.

Measurements of core temperature in spontaneously hypertensive rats by radiotelemetry

Handling and restraint can cause significant increases in core temperature. When SH [spontaneously hypertensive] rats are not stressed or disturbed, their core temperatures are not significantly different from that of normotensive WKY [Wistar-Kyoto] rats.

Year Published: 1990Topics: Biological Sampling & Physiological MeasurementAnimal Type: Rat, Rodent

Citation: Berkey, D. L., Meeuwsen, K. W., Barney, C. C. 1990. Measurements of core temperature in spontaneously hypertensive rats by radiotelemetry. American Journal of Physiology 258, R743-749.

Cortisol response of female rhesus monkeys to venipuncture in homecage versus venipuncture in restraint apparatus

All subjects were well habituated to blood collection, and it was not necessary to immobilize them; they readily presented a leg for venipuncture. In single monkeys venipunctured in the restraint apparatus, cortisol concentrations were on average 50% higher 15 minutes...

Year Published: 1990Topics: Biological Sampling & Physiological Measurement, RestraintAnimal Type: Macaque, Nonhuman Primate

Citation: Reinhardt, V., Cowley, D., Scheffler, J. et al. 1990. Cortisol response of female rhesus monkeys to venipuncture in homecage versus venipuncture in restraint apparatus. Journal of Medical Primatology 19, 601-606.

New methodology for measuring blood pressure in awake baboons with use of behavioral training techniques

Training protocols are described to ensure cooperation of 10 single-housed baboons during blood pressure measurement in the homecage.

Year Published: 1990Topics: Animal Training, Biological Sampling & Physiological MeasurementAnimal Type: Baboon, Nonhuman Primate

Citation: Turkkan, J. S. 1990. New methodology for measuring blood pressure in awake baboons with use of behavioral training techniques. Journal of Medical Primatology 19, 455-466.

Impact of venipuncture on physiological research conducted in conscious macaques

A survey of 397 publications dealing with macaques was conducted. Stress-sensitive physiological data collected during venipuncture were evaluated in 58 reports. Despite of the fact that venipuncture often is a stressful event for research animals, 81% of the studies did...

Year Published: 1991Topics: Biological Sampling & Physiological Measurement, RestraintAnimal Type: Macaque, Nonhuman Primate

Citation: Reinhardt, V. 1991. Impact of venipuncture on physiological research conducted in conscious macaques. Journal of Experimental Animal Science 34(5-6), 212-217.

Avoiding undue cortisol responses to venipuncture in adult male rhesus macaques

Six adult rhesus males were: a) habituated to actively cooperate during venipuncture away from the homecage in a treatment squeeze cage, and b) trained to actively cooperate during venipuncture in the homecage.The magnitude of cortisol increase was significant when the...

Year Published: 1991Topics: Animal Training, Biological Sampling & Physiological MeasurementAnimal Type: Macaque, Nonhuman Primate

Citation: Reinhardt, V., Cowley, D., Eisele, S. et al. 1991. Avoiding undue cortisol responses to venipuncture in adult male rhesus macaques. Animal Technology 42, 83-86.

Training adult male rhesus monkeys to actively cooperate during in-homecage venipuncture

A training technique is described for ensuring the active cooperation of 10 pair-housed and 5 single-housed adult male rhesus monkeys during in-homecage venipuncture. Mean cumulative training time investment per male was 40 minutes. Once trained, all males cooperated during in-homecage...

Year Published: 1991Topics: Animal Training, Biological Sampling & Physiological MeasurementAnimal Type: Macaque, Nonhuman Primate

Citation: Reinhardt, V. 1991. Training adult male rhesus monkeys to actively cooperate during in-homecage venipuncture. Animal Technology 42, 11-17.

In-homecage blood collection from conscious stumptailed macaques

Six adult female stumptailed macaques (Macaca aretoides) were trained within a two week period to actively co-operate during in-homecage venipuncture rather than in a restraint apparatus away from the homecage. The training was based on consistent firmness, gentleness and patience....

Year Published: 1992Topics: Animal Training, Biological Sampling & Physiological MeasurementAnimal Type: Macaque, Nonhuman Primate

Citation: Reinhardt, V., Cowley, D. 1992. In-homecage blood collection from conscious stumptailed macaques. Animal Welfare 1(4), 249-255.

Improved handling of experimental rhesus monkeys

The significant increase of serum cortisol concentration associated with involuntary manual or mechanical restraint during venipuncture was absent in females who were trained to voluntarily cooperate during the procedure in the homecage. The present findings indicate that training rhesus monkeys...

Year Published: 1992Topics: Animal Training, Biological Sampling & Physiological MeasurementAnimal Type: Macaque, Nonhuman Primate

Citation: Reinhardt, V. 1992. Improved handling of experimental rhesus monkeys. In: The Inevitable Bond. Examining Scientist-Animal Interactions. Davis, H., Balfour, A. D. (eds), 171-177. Cambridge University Press, Cambridge, UK.

Removal of blood from laboratory mammals and birds

Since stress may cause physiological reactions which may affect the research, the method of blood sampling used should be checked for any associated changes. ... It is obviously in the interests of good science, as well as of animal welfare,...

Year Published: 1993Topics: Biological Sampling & Physiological MeasurementAnimal Type: All/General

Citation: Morton, D. B., Abbot, D., Barclay, R. et al. 1993. Removal of blood from laboratory mammals and birds. Laboratory Animals 27, 1-22.