Biological Sampling & Physiological Measurement

Body temperature measurement in mice during acute illness: Implantable temperature transponder versus surface infrared thermometry

Body temperature is a valuable parameter in determining the wellbeing of laboratory animals. However, using body temperature to refine humane endpoints during acute illness generally lacks comprehensiveness and exposes to inter-observer bias. Here we compared two methods to assess body...

Year Published: 2018Topics: Biological Sampling & Physiological Measurement, Welfare AssessmentAnimal Type: Mouse, Rodent

Citation: Mei, J., Riedel, N., Grittner, U. et al. 2018. Body temperature measurement in mice during acute illness: Implantable temperature transponder versus surface infrared thermometry. Scientific Reports 8, 3526.

Quantification of hair cortisol concentration in common marmosets (Callithrix jacchus) and tufted capuchins (Cebus apella)

Quantifying cortisol concentration in hair is a non‐invasive biomarker of long‐term hypothalamic‐pituitary‐adrenal (HPA) activation, and thus can provide important information on laboratory animal health. Marmosets (Callithrix jacchus) and capuchins (Cebus apella) are New World primates increasingly used in biomedical and...

Year Published: 2018Topics: Biological Sampling & Physiological MeasurementAnimal Type: Capuchin, Marmoset, Nonhuman Primate

Citation: Phillips, K.A., Tukan, A. N., Rigodanzo, A. D. et al. 2018. Quantification of hair cortisol concentration in common marmosets (Callithrix jacchus) and tufted capuchins (Cebus apella). American Journal of Primatology 80(7), e22879.

Using a cageside device for testing glycosylated hemoglobin in cynomolgus macaques (Macaca fascicularis)

Recording an accurate body temperature is important to assess an animal's health status. We compared temperature data from sedated cynomolgus macaques (Macaca fascicularis) to evaluate differences between rectal, infrared (inguinal and chest), and implanted telemetry techniques with the objective of...

Year Published: 2017Topics: Biological Sampling & Physiological MeasurementAnimal Type: Macaque, Nonhuman Primate

Citation: Johnston, J. M., Wilson, J. M., Smith, A. L. et al. 2017. Using a cageside device for testing glycosylated hemoglobin in cynomolgus macaques (Macaca fascicularis). JAALAS 56(1), 90-94.

Comparison of saliva collection methods for the determination of salivary cortisol levels in rhesus macaques (Macaca mulatta), cynomolgus macaques (Macaca fascicularis), and African green monkeys (Chlorocebus aethiops)

The ability to quickly and accurately determine cortisol as a biomarker for stress is a valuable tool in assessing the wellbeing of NHP. In this study, 2 methods of collecting saliva (a commercial collection device and passive drool) and the...

Year Published: 2017Topics: Biological Sampling & Physiological MeasurementAnimal Type: Macaque, Nonhuman Primate, Vervet (African Green Monkey)

Citation: Rapp-Santos, K. J., Altamura, L. A., Norris, S. L. et al. 2017. Comparison of saliva collection methods for the determination of salivary cortisol levels in rhesus macaques (Macaca mulatta), cynomolgus macaques (Macaca fascicularis), and African green monkeys (Chlorocebus aethiops). JAALAS 56(2), 181-189.

Techniques for nonterminal blood sampling in black-tailed prairie dogs (Cynomys ludovicianus)

Black-tailed prairie dogs (Cynomys ludovicianus) are used as an animal model for research on gallbladder stones and several infectious diseases. A comprehensive, instructive resource regarding the appropriate techniques for venipuncture and collection of nonterminal blood samples in this species has...

Year Published: 2017Topics: Biological Sampling & Physiological MeasurementAnimal Type: Other Rodent, Rodent

Citation: Head, V., Eshar, D., Nau, M. R. 2017. Techniques for nonterminal blood sampling in black-tailed prairie dogs (Cynomys ludovicianus). JAALAS 56(2), 210-213.

Comparing phlebotomy by tail tip amputation, facial vein puncture, and tail vein incision in C57BL/6 mice by using physiologic and behavioral metrics of pain and distress

Tail tip amputation with minimal restraint is not widely used for mouse phlebotomy. In part, this infrequency may reflect policies influenced by tail tip amputation procedures for genotyping, which involve greater handling and tissue removal. To assess tail tip amputation...

Year Published: 2017Topics: Biological Sampling & Physiological MeasurementAnimal Type: Mouse, Rodent

Citation: Moore, E. S., Cleland, T. A., Williams, W. O. et al. 2017. Comparing phlebotomy by tail tip amputation, facial vein puncture, and tail vein incision in C57BL/6 mice by using physiologic and behavioral metrics of pain and distress. JAALAS 56(3), 307-317.

Hydrophobic sand versus metabolic cages: A comparison of urine collection methods for rats (Rattus norvegicus)

A common method for urine collection from rats requires the use of a metabolic cage, thus exposing animals to extended periods of isolation in an unfamiliar cage with a wire-mesh floor. A new method involving hydrophobic sand, a material more...

Year Published: 2018Topics: Biological Sampling & Physiological MeasurementAnimal Type: Rat, Rodent

Citation: Hoffman, J. F., Fan, A. X., Neuendorf, E. H. et al. 2018. Hydrophobic sand versus metabolic cages: A comparison of urine collection methods for rats (Rattus norvegicus). JAALAS 57(1), 51-57.

Comparison of direct and indirect methods of measuring arterial blood pressure in healthy male rhesus macaques (Macaca mulatta)

Blood pressure is a critical parameter for evaluating cardiovascular health, assessing effects of drugs and procedures, monitoring physiologic status during anesthesia, and making clinical decisions. The placement of an arterial catheter is the most direct and accurate method for measuring...

Year Published: 2018Topics: Biological Sampling & Physiological MeasurementAnimal Type: Macaque, Nonhuman Primate

Citation: France, L. K., Vermillion, M. S., Garrett, C. M. 2018. Comparison of direct and indirect methods of measuring arterial blood pressure in healthy male rhesus macaques (Macaca mulatta). JAALAS 57(1), 64-69.

Comparison of serial blood collection by facial vein and retrobulbar methods in C57BL/6 mice

Many biomedical research protocols for mouse models involve serial blood collection and analysis. Two common techniques for serial blood collection in this species are the retrobulbar (RB, also called retroorbital) and facial vein (FV) methods. However, previous studies comparing these...

Year Published: 2018Topics: Biological Sampling & Physiological MeasurementAnimal Type: Mouse, Rodent

Citation: Frohlich, J. R., Alarcón, C. N., Toarmino, C. R. et al. 2018. Comparison of serial blood collection by facial vein and retrobulbar methods in C57BL/6 mice. JAALAS 57(4), 382-391.

The effect of early life experience, environment, and genetic factors on spontaneous home-cage aggression-related wounding in male C57BL/6 mice

Aggression is a major welfare issue in mice, particularly when mice unfamiliar to each other are first placed in cages, as happens on receipt from a vendor, and following cage cleaning. Injuries from aggression are the second leading cause of...

Year Published: 2017Topics: Biological Sampling & Physiological Measurement, Husbandry & Management, Social Housing & CompanionshipAnimal Type: Mouse, Rodent

Citation: Gaskill, B. N., Stottler, A. M., Garner, J. P. et al. 2017. The effect of early life experience, environment, and genetic factors on spontaneous home-cage aggression-related wounding in male C57BL/6 mice. Lab Animal 46(4), 176-184.