Biological Sampling & Physiological Measurement

Non-lethal sampling for stable isotope analysis of pike Esox lucius: How mucus, scale and fin tissue compare to muscle

Stable isotope analysis (SIA) was used to examine the isotopic relationships between dorsal muscle and fin, scale and epidermal mucus in pike Esox lucius. δ13C and δ15N varied predictably within each tissue pairing, with conversion factors calculated for the surrogate...

Year Published: 2019Topics: Biological Sampling & Physiological MeasurementAnimal Type: Fish, Other Fish

Citation: Winter, E. R., Nyqvist, M., Britton, J. R. 2019. Non-lethal sampling for stable isotope analysis of pike Esox lucius: How mucus, scale and fin tissue compare to muscle. Journal of Fish Biology 95(3), 956-958.

Time-dependent pathologic and inflammatory consequences of various blood sampling techniques in mice

We compared 6 frequently used mouse blood-sampling methods (lateral tail incision; tail-tip amputation; sublingual, submandibular, and saphenous vein puncture; and retrobulbar sinus puncture during isoflurane anesthesia) with regard to induction of local and systemic inflammation, stomach contents, weight changes, and...

Year Published: 2019Topics: Biological Sampling & Physiological MeasurementAnimal Type: Mouse, Rodent

Citation: Sørensen, D. B., Metzdorff, S. B., Jensen, L. K. et al. 2019. Time-dependent pathologic and inflammatory consequences of various blood sampling techniques in mice. JAALAS 58(3), 362-372.

Association of Primate Veterinarians’ Position Statement: Cerebrospinal fluid aspiration for nonhuman primates in biomedical research

The Association of Primate Veterinarians (APV) recognizes that CSF collection may be required for both clinical and research purposes in nonhuman primates (NHP). Because there are inherent risks associated with the technique, the laboratory animal veterinarian should determine the need...

Year Published: 2019Topics: Biological Sampling & Physiological MeasurementAnimal Type: Baboon, Capuchin, Chimpanzee, Lemur, Macaque, Marmoset, Nonhuman Primate, Other Nonhuman Primate, Owl Monkey, Squirrel Monkey, Vervet (African Green Monkey)

Citation: Association of Primate Veterinarians. 2019. Association of Primate Veterinarians' Position Statement: Cerebrospinal fluid aspiration for nonhuman primates in biomedical research. JAALAS 58(4), 428-429.

Minimally invasive lumbar port system for the collection of cerebrospinal fluid from rhesus macaques (Macaca mulatta)

Biomedical translational research frequently incorporates collection of CSF from NHP, because CSF drug levels are used as a surrogate for CNS tissue penetration in pharmacokinetic and dynamic studies. Surgical placement of a CNS ventricular catheter reservoir for CSF collection is...

Year Published: 2016Topics: Biological Sampling & Physiological MeasurementAnimal Type: Macaque, Nonhuman Primate

Citation: MacAllister, R. P., Lester McCully, C. M., Bacher, J. et al. 2016. Minimally invasive lumbar port system for the collection of cerebrospinal fluid from rhesus macaques (Macaca mulatta). Comparative Medicine 66(4), 349–352.

Non-invasive stress monitoring and temperament of chestnut-bellied seed-finch (Passeriformes, Thraupidae)

Songbirds are kept as pets around the world, and bird-singing contests occur in several countries. However, there is a lack of scientific support for songbird welfare and stress monitoring. Thus, we aimed to validate the measurement of glucocorticoid metabolites (GCM)...

Year Published: 2019Topics: Biological Sampling & Physiological Measurement, Welfare AssessmentAnimal Type: Bird, Finch

Citation: Moyano Barbosa, H., Gama Nogueira-Filho, S. L., Nogueira de Morais, R. et al. 2019. Non-invasive stress monitoring and temperament of chestnut-bellied seed-finch (Passeriformes, Thraupidae). Applied Animal Behaviour Science 220, 104859.

Changes in saliva analytes correlate with horses’ behavioural reactions to an acute stressor: A pilot study

Acute stress induces an array of behavioural reactions in horses that vary between individuals. Attempts to relate behavioural patterns and physiological responses have not always given clear-cut results. Here, we measured the changes in a panel of salivary components: salivary...

Year Published: 2019Topics: Biological Sampling & Physiological Measurement, Welfare AssessmentAnimal Type: Equine

Citation: Contreras-Aguilar, M. D., Henry, S., Coste, C. et al. 2019. Changes in saliva analytes correlate with horses' behavioural reactions to an acute stressor: A pilot study. Animals 9(11), 993.

Infrared thermography—A non-invasive method of measuring respiration rate in calves

Respiration rate (RR) is commonly used to assess states of cattle health and welfare such as pain, stress and disease. Traditionally, RR is measured by counting flank movements, a method often considered to be labour-intensive and impractical. This study investigated...

Year Published: 2019Topics: Biological Sampling & Physiological MeasurementAnimal Type: Cattle

Citation: Lowe, G., Sutherland, M., Waas, J. et al. 2019. Infrared thermography—A non-invasive method of measuring respiration rate in calves. Animals 9(8), 535.

Characterization of the normal portal and hepatic blood flow of adult Holstein-Friesian cows

Knowledge of physiological portal and hepatic blood flow in cattle is essential for the use of Doppler ultrasound for diagnostic purposes. In this paper, we describe a protocol for the systematic ultrasonography evaluation of the portal and hepatic system in...

Year Published: 2019Topics: Biological Sampling & Physiological MeasurementAnimal Type: Cattle

Citation: Barreiro-Vázquez, J. D., Miranda, M., Barreiro-Vilanova, M. I. et al. 2019. Characterization of the normal portal and hepatic blood flow of adult Holstein-Friesian cows. Animals 9(6), 386.

Noninvasive cardiac psychophysiology as a tool for translational science with marmosets

The importance of marmosets for comparative and translational science has grown in recent years because of their relatively rapid development, birth cohorts of twins, family social structure, and genetic tractability. Despite this, they remain understudied in investigations of affective processes....

Year Published: 2019Topics: Biological Sampling & Physiological MeasurementAnimal Type: Macaque, Nonhuman Primate

Citation: Murphy, A. M., Ross, C. N., Bliss‐Moreau, E. 2019. Noninvasive cardiac psychophysiology as a tool for translational science with marmosets. American Journal of Primatology 81(9), e23037.

Thermography as a non-invasive measure of stress and fear of humans in sheep

No data have been published on the use of infrared thermography (IRT) to evaluate sheep emotions. We assessed whether this technique can be used as a non-invasive measure of negative emotions. Two voluntary animal approach (VAA) tests were conducted (and...

Year Published: 2018Topics: Biological Sampling & Physiological Measurement, Welfare AssessmentAnimal Type: Sheep

Citation: Cannas, S., Palestrini, C., Canali, E. et al. 2018. Thermography as a non-invasive measure of stress and fear of humans in sheep. Animals 8(9), 146.