Biological Sampling & Physiological Measurement

Conscious urine sampling and quantification from indwelling catheterization of female Yucatan swine

In addition to routine diagnostic testing of urine for health status, data from urine collection is often necessary to support various nutritional, metabolic, biochemical, and physiologic studies. Collection methods can be stressful to the animal (manual restraint), may require sedation...

Year Published: 2019Topics: Biological Sampling & Physiological Measurement, Cannulation, Catheterization, & IntubationAnimal Type: Pig

Citation: Bogins, C., Beale, C. 2019. Conscious urine sampling and quantification from indwelling catheterization of female Yucatan swine. Laboratory Animal Science Professional 7(3) (September), 44-46.

Saliva sampling in dogs: How to select the most appropriate procedure for your study

Saliva sampling is an easy, noninvasive method to assess short-term physiological changes, and as such is a valuable addition to behavioral studies in dogs. Different methods for collecting saliva samples in dogs have been reported in literature, and there appears...

Year Published: 2019Topics: Biological Sampling & Physiological MeasurementAnimal Type: Dog

Citation: Lensen, C. M. M., Moons, C. P. H., Diederich, C. 2015. Saliva sampling in dogs: How to select the most appropriate procedure for your study. Journal of Veterinary Behavior 10(6), 504-512.

Minimally-invasive, microneedle-array extraction of interstitial fluid for comprehensive biomedical applications: Transcriptomics, proteomics, metabolomics, exosome research, and biomarker identification

Interstitial fluid (ISF) has recently garnered interest as a biological fluid that could be used as an alternate to blood for biomedical applications, diagnosis, and therapy. ISF extraction techniques are promising because they are less invasive and less painful than...

Year Published: 2018Topics: Biological Sampling & Physiological MeasurementAnimal Type: Rat, Rodent

Citation: Taylor, R. M., Miller, P. R., Ebrahimi, P. et al. 2018. Minimally-invasive, microneedle-array extraction of interstitial fluid for comprehensive biomedical applications: Transcriptomics, proteomics, metabolomics, exosome research, and biomarker identification. Laboratory Animals 52(5), 526–530.

Measuring cortisol, the major stress hormone in fishes

Stress in teleosts is an increasingly studied topic because of its interaction with growth, reproduction, immune system and ultimately fitness of the animal. Whether it is for evaluating welfare in aquaculture, adaptive capacities in fish ecology, or to investigate effects...

Year Published: 2019Topics: Biological Sampling & Physiological MeasurementAnimal Type: Fish, Other Fish, Salmon, Tilapia, Trout, Zebrafish

Citation: McKenzie, D. J., Sadoul, B., Geffroy, B. 2019. Measuring cortisol, the major stress hormone in fishes. Journal of Fish Biology 94(4), 540-555.

Non-lethal sampling for stable isotope analysis of pike Esox lucius: How mucus, scale and fin tissue compare to muscle

Stable isotope analysis (SIA) was used to examine the isotopic relationships between dorsal muscle and fin, scale and epidermal mucus in pike Esox lucius. δ13C and δ15N varied predictably within each tissue pairing, with conversion factors calculated for the surrogate...

Year Published: 2019Topics: Biological Sampling & Physiological MeasurementAnimal Type: Fish, Other Fish

Citation: Winter, E. R., Nyqvist, M., Britton, J. R. 2019. Non-lethal sampling for stable isotope analysis of pike Esox lucius: How mucus, scale and fin tissue compare to muscle. Journal of Fish Biology 95(3), 956-958.

Time-dependent pathologic and inflammatory consequences of various blood sampling techniques in mice

We compared 6 frequently used mouse blood-sampling methods (lateral tail incision; tail-tip amputation; sublingual, submandibular, and saphenous vein puncture; and retrobulbar sinus puncture during isoflurane anesthesia) with regard to induction of local and systemic inflammation, stomach contents, weight changes, and...

Year Published: 2019Topics: Biological Sampling & Physiological MeasurementAnimal Type: Mouse, Rodent

Citation: Sørensen, D. B., Metzdorff, S. B., Jensen, L. K. et al. 2019. Time-dependent pathologic and inflammatory consequences of various blood sampling techniques in mice. JAALAS 58(3), 362-372.

Association of Primate Veterinarians’ Position Statement: Cerebrospinal fluid aspiration for nonhuman primates in biomedical research

The Association of Primate Veterinarians (APV) recognizes that CSF collection may be required for both clinical and research purposes in nonhuman primates (NHP). Because there are inherent risks associated with the technique, the laboratory animal veterinarian should determine the need...

Year Published: 2019Topics: Biological Sampling & Physiological MeasurementAnimal Type: Baboon, Capuchin, Chimpanzee, Lemur, Macaque, Marmoset, Nonhuman Primate, Other Nonhuman Primate, Owl Monkey, Squirrel Monkey, Vervet (African Green Monkey)

Citation: Association of Primate Veterinarians. 2019. Association of Primate Veterinarians' Position Statement: Cerebrospinal fluid aspiration for nonhuman primates in biomedical research. JAALAS 58(4), 428-429.

Minimally invasive lumbar port system for the collection of cerebrospinal fluid from rhesus macaques (Macaca mulatta)

Biomedical translational research frequently incorporates collection of CSF from NHP, because CSF drug levels are used as a surrogate for CNS tissue penetration in pharmacokinetic and dynamic studies. Surgical placement of a CNS ventricular catheter reservoir for CSF collection is...

Year Published: 2016Topics: Biological Sampling & Physiological MeasurementAnimal Type: Macaque, Nonhuman Primate

Citation: MacAllister, R. P., Lester McCully, C. M., Bacher, J. et al. 2016. Minimally invasive lumbar port system for the collection of cerebrospinal fluid from rhesus macaques (Macaca mulatta). Comparative Medicine 66(4), 349–352.

Non-invasive stress monitoring and temperament of chestnut-bellied seed-finch (Passeriformes, Thraupidae)

Songbirds are kept as pets around the world, and bird-singing contests occur in several countries. However, there is a lack of scientific support for songbird welfare and stress monitoring. Thus, we aimed to validate the measurement of glucocorticoid metabolites (GCM)...

Year Published: 2019Topics: Biological Sampling & Physiological Measurement, Welfare AssessmentAnimal Type: Bird, Finch

Citation: Moyano Barbosa, H., Gama Nogueira-Filho, S. L., Nogueira de Morais, R. et al. 2019. Non-invasive stress monitoring and temperament of chestnut-bellied seed-finch (Passeriformes, Thraupidae). Applied Animal Behaviour Science 220, 104859.

Changes in saliva analytes correlate with horses’ behavioural reactions to an acute stressor: A pilot study

Acute stress induces an array of behavioural reactions in horses that vary between individuals. Attempts to relate behavioural patterns and physiological responses have not always given clear-cut results. Here, we measured the changes in a panel of salivary components: salivary...

Year Published: 2019Topics: Biological Sampling & Physiological Measurement, Welfare AssessmentAnimal Type: Equine

Citation: Contreras-Aguilar, M. D., Henry, S., Coste, C. et al. 2019. Changes in saliva analytes correlate with horses' behavioural reactions to an acute stressor: A pilot study. Animals 9(11), 993.