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Habituation with apparatus and group testing improves assessment of fish preferences

Preference tests are commonly used to assess fish behavior and cognition in several research fields. This study aimed to investigate how fish perform in a preference test involving extended habituation to the apparatus, which was expected to reduce stress. We...

Year Published: 2025Topics: Behavioral & Cognitive TestingAnimal Type: Fish, Other Fish

Citation: Varracchio, C., Paci, F. P., Bertolucci, C. et al. 2025. Habituation with apparatus and group testing improves assessment of fish preferences. Journal of Fish Biology 107(4), 1149–1158.

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Interaction with enrichment objects depends on color and increases after feeding in some elasmobranchs but not others

Environmental enrichment is important for the long-term wellbeing of captive organisms and is an understudied topic in aquatic taxa, particularly ectothermic organisms such as elasmobranchs. This study examines how individual elasmobranchs use environmental enrichment objects before and after feeding. The...

Year Published: 2025Topics: Environmental EnrichmentAnimal Type: Fish, Shark & Ray

Citation: Smith, A. B., Landesman, J., Williamson, L. et al. 2025. Interaction with enrichment objects depends on color and increases after feeding in some elasmobranchs but not others. Applied Animal Behaviour Science 292, 106829.

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Non-invasive ultrasound measurements for determination of hepato-somatic index in Greenland halibut (Reinhardtius hippoglossoides)

Hepato-somatic index (HSI) is widely used to indicate fish body condition and level of energy reserves related to feeding/physiology and reproduction. HSI is traditionally obtained by dissection and measurement of liver mass versus body mass, but ultrasound technology may provide...

Year Published: 2025Topics: Welfare AssessmentAnimal Type: Fish, Other Fish

Citation: Schilling, J. W., Hussey, N. E., Hedges, K. J. et al. 2025. Non-invasive ultrasound measurements for determination of hepato-somatic index in Greenland halibut (Reinhardtius hippoglossoides). Journal of Fish Biology 107(4), 1313–1322.

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Methods of humane killing of laboratory fish: FELASA Working Group recommendations

As commissioned by the Federation of European Laboratory Animal Science Associations, these working group recommendations define the requirements to achieve the humane killing of fish, compare methods of killing, recommend methods of killing depending on context, and detail protocols leading...

Year Published: 2025Topics: EuthanasiaAnimal Type: Fish, Zebrafish

Citation: Mocho, J.-P., Blasco, J. R., Lundegaard, P. R. et al. 2025. Methods of humane killing of laboratory fish: FELASA Working Group recommendations. Laboratory Animals 59(5), 599–613.

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Blue light preconditioning as a novel strategy for reducing transport stress in zebrafish

In recent years, zebrafish have become increasingly important in experimental sciences, with transportation being a critical step in their use. However, the vibrations during transportation often induce stress responses in the fish, impairing their physiological functions and even leading to...

Year Published: 2025Topics: Relocation & TransportAnimal Type: Fish, Zebrafish

Citation: Huo, L., Miao, X., Zhang, X. et al. 2025. Blue light preconditioning as a novel strategy for reducing transport stress in zebrafish. Behavioural Processes 231, 105260.

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Premises for digital twins reporting on Atlantic salmon wellbeing

Many species of fish, birds and mammals commonly live in human captivity; Atlantic salmon Salmo salar is one of them. The international legal status of the welfare of captive animals is slowly developing and still requires rigorous specification. For example,...

Year Published: 2025Topics: Welfare AssessmentAnimal Type: Fish, Salmon

Citation: Giske, J., Dumitru, M. L., Enberg, K. et al. 2025. Premises for digital twins reporting on Atlantic salmon wellbeing. Behavioural Processes 226, 105163.

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Preference for complex environments in larval zebrafish

Environmental complexity is increasingly recognized as a critical factor for animal welfare and cognitive development across vertebrates. While adult zebrafish (Danio rerio) show a strong preference for complex environments, little is known about the importance of environmental complexity in larval...

Year Published: 2025Topics: Environmental EnrichmentAnimal Type: Fish, Zebrafish

Citation: Gatto, E., Agrillo, C., Dadda, M. et al. 2025. Preference for complex environments in larval zebrafish. Applied Animal Behaviour Science 292, 106788.

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Environmental enrichment with aquatic plants: Impact of the environment of choice on performance of bettas

This study aimed to evaluate the choice between environments with aquatic plants and the impact of the environment of choice on the performance of bettas (Betta splendens). First, the choice between environments with natural and artificial plants was evaluated. Adult...

Year Published: 2025Topics: Environmental EnrichmentAnimal Type: Fish, Other Fish

Citation: Bender, M., Oliveira dos Santos Neves, N., Regina Delziovo, F. et al. 2025. Environmental enrichment with aquatic plants: Impact of the environment of choice on performance of bettas. Journal of Applied Animal Welfare Science 28(4), 740–749.

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The maintenance conditions of the zebrafish (Danio rerio) breeding stock affect the survival and behavior of their offspring

The environment in which we live influences our biological traits throughout our ontogeny, being markedly significant during the beginning of our lives. Some characteristics inherited (genetically and/or epigenetically) from parents can also influence individual development, known as transgenerational effects. In...

Year Published: 2025Topics: Environmental Enrichment, Husbandry & Management, ReproductionAnimal Type: Fish, Zebrafish

Citation: Amaral, F. U. I., Pompermaier, A., Rutikoski, G. et al. 2025. The maintenance conditions of the zebrafish (Danio rerio) breeding stock affect the survival and behavior of their offspring. Applied Animal Behaviour Science 292, 106761.

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Recovery of male Siamese fighting fish (Betta splendens) after overland shipping

Ornamental fish shipped by road or rail may spend days in transit without food, leading to a reduction in somatic growth after transportation and during acclimatization. In the present study, a time-series (0, 2, 4, 6, 8, 10, and 12...

Year Published: 2025Topics: Relocation & TransportAnimal Type: Fish, Other Fish

Citation: Thongprajukaew, K., Malawa, S., Poolthajit, S. et al. 2025. Recovery of male Siamese fighting fish (Betta splendens) after overland shipping. Animals 15(14), 2156.

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