Biological Sampling & Physiological Measurement

The effect of repeated blood harvesting from pregnant mares on haematological variables

Studies have been carried out on the effect of large-volume blood harvesting from horses, but they were performed on nonpregnant horses of various breeds other than Icelandic horses. This study aimed to investigate the effect on the haematological variables indicating...

Year Published: 2024Topics: Biological Sampling & Physiological MeasurementAnimal Type: Equine

Citation: Oddsdóttir, C., Jónsdóttir, H. K., Sturludóttir, E. et al. 2024. The effect of repeated blood harvesting from pregnant mares on haematological variables. Animals 14(5), 745.

Noncontact infrared thermometer measurements offer a reasonable alternative to rectal temperature measurement in afebrile horses

OBJECTIVE To assess the repeatability of infrared thermometer temperature readings and evaluate the correlation between digital rectal temperature and infrared thermometer temperatures taken at different locations in healthy afebrile horses. ANIMALS 101 afebrile horses ≥ 1 year old. METHODS Digital...

Year Published: 2024Topics: Biological Sampling & Physiological MeasurementAnimal Type: Equine

Citation: Zobrist, C. N., Bishop, R. C., McCoy, A. M. 2024. Noncontact infrared thermometer measurements offer a reasonable alternative to rectal temperature measurement in afebrile horses. Journal of the American Veterinary Medical Association 262(6), 791–797.

The arterial blood sampling associated with PET imaging studies can lead to post-scan complications in Göttingen minipigs

When disease progression or treatment effects are investigated, pigs are frequently used for imaging studies. We recently showed that prolonged anesthesia with intensive blood sampling and road transportation affect internal organs in non-recovery imaging studies in domestic pigs. In the...

Year Published: 2024Topics: Biological Sampling & Physiological Measurement, Imaging & Electrophysiological RecordingAnimal Type: Pig

Citation: Alstrup, A. K. O., Thea Pinholt Lillethorup, Landau, A. M. et al. 2024. The arterial blood sampling associated with PET imaging studies can lead to post-scan complications in Göttingen minipigs. Scandinavian Journal of Laboratory Animal Science 50, 6–12.

Analytical and biological validation of a noninvasive measurement of glucocorticoid metabolites in feces of Geoffroy’s spider monkeys (Ateles geoffroyi)

We report on an analytical and biological validation of a commercial cortisol enzyme immunoassay (EIA) to measure glucocorticoids (GC) in feces of Geoffroy's spider monkeys (Ateles geoffroyi). Validation of endocrinological methods for each sample matrix and study species is crucial...

Year Published: 2024Topics: Biological Sampling & Physiological MeasurementAnimal Type: Nonhuman Primate, Other Nonhuman Primate

Citation: Damm, J., Aureli, F., Rangel-Negrín, A. et al. 2024. Analytical and biological validation of a noninvasive measurement of glucocorticoid metabolites in feces of Geoffroy's spider monkeys (Ateles geoffroyi). American Journal of Primatology 86(4), e23598.

Paper towel shredding as a novel, affordable, noninvasive method for detecting arousals in hibernating rodents

Many research groups explore the regulation of hibernation or compare the physiology of heterothermic mammals between the torpid and aroused, euthermic states. Current methods for monitoring torpor (for example, infrared cameras, body temperature or heart-rate telemetry, and motion sensing) are...

Year Published: 2024Topics: Biological Sampling & Physiological Measurement, Husbandry & ManagementAnimal Type: Other Rodent, Rodent

Citation: Hutchinson, A. J., Duffy, B. M., Rego, L. H. et al. 2024. Paper towel shredding as a novel, affordable, noninvasive method for detecting arousals in hibernating rodents. Lab Animal 53(5), 117–120.

Differences in fluid, electrolyte, and energy balance in C57BL/6J mice (Mus musculus) in metabolic caging at thermoneutral or standard room temperatures

The Guide for the Care and Use of Laboratory Animals recommends mice be pair or group housed and provided with nesting materials. These provisions support social interactions and are also critical for thermoregulatory behaviors such as huddling and burrowing. However,...

Year Published: 2024Topics: Biological Sampling & Physiological Measurement, Husbandry & ManagementAnimal Type: Mouse, Rodent

Citation: Lawton, S. B., Grobe, C. C., Reho, J. J. et al. 2024. Differences in fluid, electrolyte, and energy balance in C57BL/6J mice (Mus musculus) in metabolic caging at thermoneutral or standard room temperatures. JAALAS 63(2), 190–200.

Clean-catching urine from pigs: A method for collecting quality specimens for urinalysis and microbiological culturing in a laboratory environment

Urine is an important biological specimen for assessing various metabolic functions and drug clearance. In urinary tract infection research, urine is particularly important as urinary bacterial titres constitute the main diagnostic outcome for assessing the course of infection. Collecting uncontaminated...

Year Published: 2023Topics: Biological Sampling & Physiological MeasurementAnimal Type: Pig

Citation: Stærk, K., Langhorn, L., Andersen, T. E. 2023. Clean-catching urine from pigs: A method for collecting quality specimens for urinalysis and microbiological culturing in a laboratory environment. Laboratory Animals 57(3), 293–303.

Noninvasive monitoring of blood pressure and heart rate during estrous cycle phases in bormotensive Wistar–Kyoto and spontaneously hypertensive female rats

Since 2015, the National Institutes of Health has called for its funded preclinical research to include both male and female subjects. However, much of the basic animal research that has studied heart rate and blood pressure in the past has...

Year Published: 2023Topics: Biological Sampling & Physiological MeasurementAnimal Type: Rat, Rodent

Citation: Ayala-Méndez, G. X., Calderón, V. M., Zuñiga-Pimentel, T. A. et al. 2023. Noninvasive monitoring of blood pressure and heart rate during estrous cycle phases in bormotensive Wistar–Kyoto and spontaneously hypertensive female rats. JAALAS 62(3), 267–273.

Evaluation of submental blood collection in mice (Mus musculus)

The submental route is an option for nonterminal and serial blood collection in mice. This study compared the submental route to the maxillary route (also referred to as the submandibular route). The study used male CD1 and C57BL/6 strains of...

Year Published: 2023Topics: Biological Sampling & Physiological MeasurementAnimal Type: Mouse, Rodent

Citation: George, A. J., Harmsen, B. J., Ford, J. A. et al. 2023. Evaluation of submental blood collection in mice (Mus musculus). JAALAS 62(1), 92–98.

Non-invasive, real-time stress measurement: Vocalization compared with thermal imaging in kittens of the domestic cat in response to social separation

Finding tools to assess the stress response which can be easily applied, are non-invasive, reliable and measured in real time is still a relevant topic in many areas of biology. Vocal characteristics and temperature of certain body areas have been...

Year Published: 2023Topics: Biological Sampling & Physiological MeasurementAnimal Type: Cat

Citation: Szenczi, P., Jiménez Gallardo, A., Urrutia, A. et al. 2023. Non-invasive, real-time stress measurement: Vocalization compared with thermal imaging in kittens of the domestic cat in response to social separation. Behavioural Processes 213, 104955.