Biological Sampling & Physiological Measurement

Testing the level of cortisol in dogs (2025)

This paper focuses on cortisol levels in different dog matrices, emphasising the importance of non-invasive sampling for animal welfare and research ethics. The study reviewed over 18,000 articles from various databases, identifying 78 relevant studies that met the inclusion criteria....

Year Published: 2025Topics: Biological Sampling & Physiological MeasurementAnimal Type: Dog

Citation: Kaszycka, K., Goleman, M., Krupa, W. 2025. Testing the level of cortisol in dogs. Animals 15(9), 1197.

Enhancing stress assessment in sledge reindeer (Rangifer tarandus): A pilot study on infrared thermal imaging and its opportunities for advancement as a welfare assessment tool

Measuring immediate physiological stress responses in animals can be challenging; saliva and blood sampling, while invasive, may also generate confounding stress responses, and equipping animals with heart rate sensors is not always feasible. Nevertheless, emerging technologies offer a non-invasive and...

Year Published: 2024Topics: Biological Sampling & Physiological Measurement, Human-Animal InteractionAnimal Type: Other Animal

Citation: Liehrmann, O., Ollila, A., Lummaa, V. et al., 2024. Enhancing stress assessment in sledge reindeer (Rangifer tarandus): A pilot study on infrared thermal imaging and its opportunities for advancement as a welfare assessment tool. Animal Behavior and Cognition 11(3), 293-304.

A new research tool for use in sharks and rays: Relevance of reproductive hormone levels in the skin of small-spotted catshark (Scyliorhinus canicula) (2025)

The present study marks a significant step forward in validating the use of skin biopsies for measuring reproductive steroid hormones in sharks. Given the conservation concerns surrounding many chondrichthyan species and the growing emphasis on adhering to the 3R principles...

Year Published: 2025Topics: Biological Sampling & Physiological MeasurementAnimal Type: Fish, Shark & Ray

Citation: Carbajal, A., Lobato, I. G., Agustí, C. et al. 2025. A new research tool for use in sharks and rays: Relevance of reproductive hormone levels in the skin of small-spotted catshark (Scyliorhinus canicula) (2025). Animals 15(5), 762.

Long term ear and saphenous cannulation to perform blood sampling over longer periods using the Seldinger technique (2024)

The standard practice to collect a blood sample from a Minipig is to invert the animal onto its back and into a cradle. The blood sample is then collected via jugular venepuncture. This technique can cause large amounts of stress...

Year Published: 2024Topics: Biological Sampling & Physiological Measurement, Cannulation, Catheterization, & IntubationAnimal Type: Pig

Citation: Bailey, G., Simpson, T., Sharpe, J. 2024. Long term ear and saphenous cannulation—To perform blood sampling over longer periods using the Seldinger technique. Animal Technology and Welfare 23(3), 217–218.

Non-invasive sampling methods for genotyping: Improvements towards the 4r principle

In research involving genetically modified mice which is usually to perform genotyping using invasive ear or tail biopsies in 92% of cases, there is a growing shift towards non-invasive sampling techniques such as oral swabs, hair, faeces and tears. Our...

Year Published: 2024Topics: Biological Sampling & Physiological Measurement, Identification MethodAnimal Type: Mouse, Rabbit, Rodent

Citation: Thasian-Sivarajah, S., Mohr, R., Walter, M. et al. 2024. Non-invasive sampling methods for genotyping: Improvements towards the 4r principle. Animal Technology and Welfare 23(2), 133–136.

A novel approach for contactless heart rate monitoring from pet facial videos

Introduction: Monitoring the heart rate (HR) of pets is challenging when contact with a conscious pet is inconvenient, difficult, injurious, distressing, or dangerous for veterinarians or pet owners. However, few established, simple, and non-invasive techniques for HR measurement in pets...

Year Published: 2024Topics: Biological Sampling & Physiological MeasurementAnimal Type: Cat, Dog

Citation: Hu, R., Gao, Y., Peng, G. et al. 2024. A novel approach for contactless heart rate monitoring from pet facial videos. Frontiers in Veterinary Science 11.

Novel method to investigate thermal exchange rates in small, terrestrial ectotherms: A proof-of-concept on the gecko Tarentola mauritanica

Thermoregulating ectotherms may resort to different external heat sources to modulate their body temperature through an array of behavioural and physiological adaptations which modulate heat exchange with the environment and its distribution across the animal's body. Even small-bodied animals are...

Year Published: 2024Topics: Biological Sampling & Physiological MeasurementAnimal Type: Lizard, Reptile

Citation: Mochales-Riaño, G., Barroso, F. M., Marques, V. et al. 2024. Novel method to investigate thermal exchange rates in small, terrestrial ectotherms: A proof-of-concept on the gecko Tarentola mauritanica. PLOS ONE 19(12), e0316283.

Use of the oxygen reserve index/FiO2 as a non-invasive index to estimate venous admixture in anesthetized dogs

The oxygen reserve index (ORi) is a novel, non-invasive parameter that estimates arterial oxygen partial pressure (PaO2) during hyperoxia when the fraction of inspired oxygen (FiO2) is elevated. This study aimed to assess the utility of the ORi/FiO2 ratio as...

Year Published: 2025Topics: Anesthesia & Sedation, Biological Sampling & Physiological MeasurementAnimal Type: Dog

Citation: Bellini, L., Maney, J. K., Zanusso, F. et al. 2025. Use of the oxygen reserve index/FiO2 as a non-invasive index to estimate venous admixture in anesthetized dogs. Frontiers in Veterinary Science 11.

Effects of handling and surgical implant on juvenile wild trout: Physiological and behavioral impact

Fish involved in telemetry studies are usually handled, anaesthetized, and subjected to internal tag implantation, all of which have the potential to disrupt the fish's physiology, migratory patterns, food-seeking behavior, growth, and survival. As fish tagging is a widespread standard...

Year Published: 2024Topics: Biological Sampling & Physiological Measurement, HandlingAnimal Type: Fish, Trout

Citation: Makaras, T., Stankevičiūtė, M. 2024. Effects of handling and surgical implant on juvenile wild trout: Physiological and behavioral impact. Journal of Fish Biology 105(1), 265–278.

First noncontact millimeter-wave radar measurement of heart rate in great apes: Validation in chimpanzees (Pan troglodytes)

Heart rate is a crucial vital sign and a valuable indicator for assessing the physical and psychological condition of a target animal. Heart rate contributes to (1) fundamental information for cognitive research, (2) an indicator of psychological and physical stress,...

Year Published: 2024Topics: Biological Sampling & Physiological MeasurementAnimal Type: Chimpanzee, Nonhuman Primate

Citation: Matsumoto, T., Iwata, I., Sakamoto, T. et al. 2024. First noncontact millimeter-wave radar measurement of heart rate in great apes: Validation in chimpanzees (Pan troglodytes). American Journal of Primatology 86(8), e23633.