Biological Sampling & Physiological Measurement

Factors affecting initial training success of blood glucose testing in captive chimpanzees (Pan troglodytes)

Type 2 diabetes can be a problem for captive chimpanzees. Accurate blood glucose (BG) readings are necessary to monitor and treat this disease. Thus, obtaining voluntary samples from primates through positive reinforcement training (PRT) is critical. The current study assessed...

Year Published: 2014Topics: Animal Training, Biological Sampling & Physiological Measurement, Disease/Experimental ModelAnimal Type: Chimpanzee, Nonhuman Primate

Citation: Reamer, L. A., Haller, R. H., Thiele, E. J. et al. 2014. Factors affecting initial training success of blood glucose testing in captive chimpanzees (Pan troglodytes). Zoo Biology 33(3), 212-220.

Abnormal behavior and associated risk factors in captive baboons (Papio hamadryas spp

Abnormal behavior, ranging from motor stereotypies to self‐injurious behavior, has been documented in captive nonhuman primates, with risk factors including nursery rearing, single housing, and veterinary procedures. Much of this research has focused on macaque monkeys; less is known about...

Year Published: 2014Topics: Abnormal/Problematic Behavior, Biological Sampling & Physiological Measurement, Social Housing & CompanionshipAnimal Type: Baboon, Nonhuman Primate

Citation: Lutz, C. K., Williams, P. C., Sharp, R. M. 2014. Abnormal behavior and associated risk factors in captive baboons (Papio hamadryas spp.). American Journal of Primatology 76(4), 355-361.

Refinement and efficiency through serial microsampling: Cost:benefit analysis

Advances in bioanalytic methods allow for accurate plasma drug level measurement in small sample volumes. Decreased sample volumes enable serial blood sample collection from one rodent, rather than terminal or sparse sampling from multiple rodents. Decreased variability in data from...

Year Published: 2014Topics: Biological Sampling & Physiological MeasurementAnimal Type: Mouse, Rodent

Citation: Bratcher, N. A., Ebert, P. A., Buck, W. et al. 2014. Refinement and efficiency through serial microsampling: Cost:benefit analysis. American Association for Laboratory Animal Science [AALAS] Meeting Official Program, 580. (Abstract #P121).

An innovative restraint device for the placement of intravenous tail vein catheters in anesthetized mice

Consistently accurate intravenous tail vein administration of radiopharmaceuticals in anesthetized mice is a critical component of successful PET/CT imaging in the preclinical setting. Injectable anesthetics are often not suitable for PET imaging and commercially available mouse restraint devices do not...

Year Published: 2014Topics: Biological Sampling & Physiological Measurement, RestraintAnimal Type: Mouse, Rodent

Citation: Tupper, T., Manning, H., Turner, D. et al. 2014. An innovative restraint device for the placement of intravenous tail vein catheters in anesthetized mice. American Association for Laboratory Animal Science [AALAS] Meeting Official Program, 584. (Abstract #P136).

Refining methods for mouse hepatic biopsy

The mouse is a commonly used species in liver research such as oncology, toxicology, and disease models. The ability to collect specimens of liver is vital to such research. Such studies require either a major surgical procedure or euthanasia to...

Year Published: 2014Topics: Biological Sampling & Physiological MeasurementAnimal Type: Mouse, Rodent

Citation: Torres, M. 2014. Refining methods for mouse hepatic biopsy. American Association for Laboratory Animal Science [AALAS] Meeting Official Program, 557-558 (Abstract #P32).

Effects of needle gauge on blood quality and potential for bruising with repeated blood collections in cynomolgus macaques

Obtaining quality blood samples while minimizing animal trauma related to venipuncture continues to be an important area of focus within the laboratory animal environment as it relates to both animal welfare and sample integrity. A review of the literature suggested...

Year Published: 2014Topics: Biological Sampling & Physiological MeasurementAnimal Type: Macaque, Nonhuman Primate

Citation: Schillaci, T. T., Bishop, B. 2014. Effects of needle gauge on blood quality and potential for bruising with repeated blood collections in cynomolgus macaques. American Association for Laboratory Animal Science [AALAS] Meeting Official Program, 609-610. (Abstract #P226).

Refining blood collection techniques to improve animal welfare and sample quality

An effort to refine rodent blood collection techniques resulted in an examination of alternative methodologies to improve animal welfare and sample quality. Disease model or repeated sample collection resulted in inadequate sample volume, occasional hemolysis, and complications including bruising, scarring,...

Year Published: 2014Topics: Biological Sampling & Physiological MeasurementAnimal Type: Mouse, Rodent

Citation: Allaire. A. C., Boule, S. 2014. Refining blood collection techniques to improve animal welfare and sample quality. American Association for Laboratory Animal Science [AALAS] Meeting Official Program, 605-606. (Abstract #P212).

Positive reinforcement methods to train chimpanzees to cooperate with urine collection

Positive reinforcement training can be used in many ways to enhance the welfare of captive primates. Training for biologic sample collection is one application of positive reinforcement training. In this study, 35 adult female chimpanzees were trained to cooperate with...

Year Published: 2015Topics: Animal Training, Biological Sampling & Physiological MeasurementAnimal Type: Chimpanzee, Nonhuman Primate

Citation: Bloomsmith, M., Kim, N., Franklin, A. et al. 2015. Positive reinforcement methods to train chimpanzees to cooperate with urine collection. JAALAS 54, 66-69.

Effects of group housing on ECG assessment in conscious cynomolgus monkeys

Assessing the cardiovascular safety of new chemical or biological entities is important during pre-clinical development. Electrocardiogram (ECG) assessments in non-human primate (NHP) toxicology studies are often made using non-invasive telemetry systems. We investigated whether ECG recording was feasible during group...

Year Published: 2015Topics: Biological Sampling & Physiological Measurement, External Bodily Equipment, Social Housing & CompanionshipAnimal Type: Macaque, Nonhuman Primate

Citation: Xing, G., Lu, J., Hu, M. et al. 2015. Effects of group housing on ECG assessment in conscious cynomolgus monkeys. Journal of Pharmacological and Toxicological Methods 75, 44-51.

Using dried blood spot sampling to improve data quality and reduce animal use in mouse pharmacokinetic studies

Traditional pharmacokinetic analysis in nonclinical studies is based on the concentration of a test compound in plasma and requires approximately 100 to 200 μL blood collected per time point. However, the total blood volume of mice limits the number of...

Year Published: 2015Topics: Biological Sampling & Physiological MeasurementAnimal Type: Mouse, Rodent

Citation: Wickremsinhe, E. R., Perkins, E. J. 2015. Using dried blood spot sampling to improve data quality and reduce animal use in mouse pharmacokinetic studies. JAALAS. 54(2), 139-144.