Biological Sampling & Physiological Measurement

Operant conditioning with laboratory beagles

There is increased regulatory emphasis on positive reinforcement training for husbandry, research procedures, and restraint devices. We practice acclimation, association, and positive reinforcement training with our beagle colony with goals to reduce stress for dogs and improve efficiency for personnel....

Year Published: 2013Topics: Animal Training, Biological Sampling & Physiological Measurement, Handling, Husbandry & ManagementAnimal Type: Dog

Citation: Savastano, G. M. 2013. Operant conditioning with laboratory beagles. American Association for Laboratory Animal Science [AALAS] Meeting Official Program, 619 (Abstract #PS57).

Validation of a refined technique for taking repeated blood samples from juvenile and adult mice

Repeated blood sampling from laboratory animals is desirable in certain experimental designs and also for reducing the number of animals used in research. Biochemical methods for analysing blood samples require only small blood volumes to be collected (typically 20-40 μL)....

Year Published: 2013Topics: Biological Sampling & Physiological MeasurementAnimal Type: Mouse, Rodent

Citation: Sadler, A. M., Bailey, S. J. 2013. Validation of a refined technique for taking repeated blood samples from juvenile and adult mice. Laboratory Animals 47(4), 316-319.

The effects of operant training on blood collection for domestic cats

The impact of operantly training domestic cats to accept jugular blood collections in a dorsally recumbent (novel) position was assessed. Cats were assigned to one of three groups: Group 1 (N = 14): no training, traditional jugular blood collection; Group...

Year Published: 2013Topics: Animal Training, Biological Sampling & Physiological MeasurementAnimal Type: Cat

Citation: Lockhart, J., Wilson, K., Lanman, C. 2013. The effects of operant training on blood collection for domestic cats. Applied Animal Behaviour Science 143(2-4), 128-134.

Hormonal stress response of laboratory mice to conventional and minimally invasive bleeding techniques

Conventional bleeding of small laboratory animals is often associated with stress and injuries that can cause haematomas, inflammation and ultimately the death of animals under investigation. Here, we used faecal glucocorticoid metabolites as an indicator of stress imposed on laboratory...

Year Published: 2013Topics: Biological Sampling & Physiological MeasurementAnimal Type: Mouse, Rodent

Citation: Voigt, C. C., Klockner, P., Touma, C. et al. 2013. Hormonal stress response of laboratory mice to conventional and minimally invasive bleeding techniques. Animal Welfare 22(4), 449-455.

Impact of blood sampling procedures on the welfare of laboratory mice

A previous study showed that blood sampling either automatically, through a catheter, or by puncture of the tail vein is less stressful than facial vein phlebotomy in mice. We decided to investigate how facial vein phlebotomy would affect animal welfare...

Year Published: 2014Topics: Biological Sampling & Physiological MeasurementAnimal Type: Mouse, Rodent

Citation: Teilmann, A. C., Madsen, A. N., Holst, B. et al. 2014. Impact of blood sampling procedures on the welfare of laboratory mice. American Association for Laboratory Animal Science [AALAS] Meeting Official Program, 600-601. (Abstract #P195).

The social buffering effect of playful handling on responses to repeated intraperitoneal injections in laboratory rats

Handling small animals for veterinary and experimental procedures can negatively affect animal wellbeing. We hypothesized that playful handling (tickling) would decrease stress associated with repeated injections in adult laboratory rats, especially those with prior tickling experience. We compared responses of...

Year Published: 2014Topics: Biological Sampling & Physiological MeasurementAnimal Type: Rat, Rodent

Citation: Cloutier, S., Wahl, K., Baker, C. et al. 2014. The social buffering effect of playful handling on responses to repeated intraperitoneal injections in laboratory rats. JAALAS 53(2), 168-173.

Factors affecting initial training success of blood glucose testing in captive chimpanzees (Pan troglodytes)

Type 2 diabetes can be a problem for captive chimpanzees. Accurate blood glucose (BG) readings are necessary to monitor and treat this disease. Thus, obtaining voluntary samples from primates through positive reinforcement training (PRT) is critical. The current study assessed...

Year Published: 2014Topics: Animal Training, Biological Sampling & Physiological Measurement, Disease/Experimental ModelAnimal Type: Chimpanzee, Nonhuman Primate

Citation: Reamer, L. A., Haller, R. H., Thiele, E. J. et al. 2014. Factors affecting initial training success of blood glucose testing in captive chimpanzees (Pan troglodytes). Zoo Biology 33(3), 212-220.

Abnormal behavior and associated risk factors in captive baboons (Papio hamadryas spp

Abnormal behavior, ranging from motor stereotypies to self‐injurious behavior, has been documented in captive nonhuman primates, with risk factors including nursery rearing, single housing, and veterinary procedures. Much of this research has focused on macaque monkeys; less is known about...

Year Published: 2014Topics: Abnormal/Problematic Behavior, Biological Sampling & Physiological Measurement, Social Housing & CompanionshipAnimal Type: Baboon, Nonhuman Primate

Citation: Lutz, C. K., Williams, P. C., Sharp, R. M. 2014. Abnormal behavior and associated risk factors in captive baboons (Papio hamadryas spp.). American Journal of Primatology 76(4), 355-361.

Refinement and efficiency through serial microsampling: Cost:benefit analysis

Advances in bioanalytic methods allow for accurate plasma drug level measurement in small sample volumes. Decreased sample volumes enable serial blood sample collection from one rodent, rather than terminal or sparse sampling from multiple rodents. Decreased variability in data from...

Year Published: 2014Topics: Biological Sampling & Physiological MeasurementAnimal Type: Mouse, Rodent

Citation: Bratcher, N. A., Ebert, P. A., Buck, W. et al. 2014. Refinement and efficiency through serial microsampling: Cost:benefit analysis. American Association for Laboratory Animal Science [AALAS] Meeting Official Program, 580. (Abstract #P121).

An innovative restraint device for the placement of intravenous tail vein catheters in anesthetized mice

Consistently accurate intravenous tail vein administration of radiopharmaceuticals in anesthetized mice is a critical component of successful PET/CT imaging in the preclinical setting. Injectable anesthetics are often not suitable for PET imaging and commercially available mouse restraint devices do not...

Year Published: 2014Topics: Biological Sampling & Physiological Measurement, RestraintAnimal Type: Mouse, Rodent

Citation: Tupper, T., Manning, H., Turner, D. et al. 2014. An innovative restraint device for the placement of intravenous tail vein catheters in anesthetized mice. American Association for Laboratory Animal Science [AALAS] Meeting Official Program, 584. (Abstract #P136).