Biological Sampling & Physiological Measurement

Refining blood collection techniques to improve animal welfare and sample quality

An effort to refine rodent blood collection techniques resulted in an examination of alternative methodologies to improve animal welfare and sample quality. Disease model or repeated sample collection resulted in inadequate sample volume, occasional hemolysis, and complications including bruising, scarring,...

Year Published: 2014Topics: Biological Sampling & Physiological MeasurementAnimal Type: Mouse, Rodent

Citation: Allaire. A. C., Boule, S. 2014. Refining blood collection techniques to improve animal welfare and sample quality. American Association for Laboratory Animal Science [AALAS] Meeting Official Program, 605-606. (Abstract #P212).

Positive reinforcement methods to train chimpanzees to cooperate with urine collection

Positive reinforcement training can be used in many ways to enhance the welfare of captive primates. Training for biologic sample collection is one application of positive reinforcement training. In this study, 35 adult female chimpanzees were trained to cooperate with...

Year Published: 2015Topics: Animal Training, Biological Sampling & Physiological MeasurementAnimal Type: Chimpanzee, Nonhuman Primate

Citation: Bloomsmith, M., Kim, N., Franklin, A. et al. 2015. Positive reinforcement methods to train chimpanzees to cooperate with urine collection. JAALAS 54, 66-69.

Effects of group housing on ECG assessment in conscious cynomolgus monkeys

Assessing the cardiovascular safety of new chemical or biological entities is important during pre-clinical development. Electrocardiogram (ECG) assessments in non-human primate (NHP) toxicology studies are often made using non-invasive telemetry systems. We investigated whether ECG recording was feasible during group...

Year Published: 2015Topics: Biological Sampling & Physiological Measurement, External Bodily Equipment, Social Housing & CompanionshipAnimal Type: Macaque, Nonhuman Primate

Citation: Xing, G., Lu, J., Hu, M. et al. 2015. Effects of group housing on ECG assessment in conscious cynomolgus monkeys. Journal of Pharmacological and Toxicological Methods 75, 44-51.

Using dried blood spot sampling to improve data quality and reduce animal use in mouse pharmacokinetic studies

Traditional pharmacokinetic analysis in nonclinical studies is based on the concentration of a test compound in plasma and requires approximately 100 to 200 μL blood collected per time point. However, the total blood volume of mice limits the number of...

Year Published: 2015Topics: Biological Sampling & Physiological MeasurementAnimal Type: Mouse, Rodent

Citation: Wickremsinhe, E. R., Perkins, E. J. 2015. Using dried blood spot sampling to improve data quality and reduce animal use in mouse pharmacokinetic studies. JAALAS. 54(2), 139-144.

Miniaturized blood sampling techniques to benefit reduction in mice and refinement in nonhuman primates: Application to bioanalysis in toxicity studies with antibody-drug conjugates

Minimizing the number of animals in regulatory toxicity studies while achieving study objectives to support the development of future medicines contributes to good scientific and ethical practices. Recent advances in technology have enabled the development of miniaturized blood sampling methods...

Year Published: 2015Topics: Biological Sampling & Physiological MeasurementAnimal Type: Baboon, Capuchin, Chimpanzee, Lemur, Macaque, Marmoset, Mouse, Nonhuman Primate, Other Nonhuman Primate, Owl Monkey, Rodent, Squirrel Monkey, Vervet (African Green Monkey)

Citation: Caron, A., Lelong, C., Pascual, M. H. et al. 2015. Miniaturized blood sampling techniques to benefit reduction in mice and refinement in nonhuman primates: Application to bioanalysis in toxicity studies with antibody-drug conjugates. JAALAS 54(2), 145-152.

Non-invasive blood pressure measurement: values, problems and applicability in the common marmoset (Callithrix jacchus)

The common marmoset (Callithrix jacchus, C. j.) is an established primate model in biomedical research and for human-related diseases. Monitoring of cardiovascular parameters including blood pressure (BP) is important for the health surveillance of these experimental animals and the quantification...

Year Published: 2015Topics: Biological Sampling & Physiological MeasurementAnimal Type: Marmoset, Nonhuman Primate

Citation: Mietsch, M., Einspanier, A. 2015. Non-invasive blood pressure measurement: values, problems and applicability in the common marmoset (Callithrix jacchus). Laboratory Animals 49(3), 241-250.

Telemetric assessment of social and single housing: Evaluation of electrocardiographic intervals in jacketed cynomolgus monkeys

Introduction: Proactive efforts to socially house laboratory animals are a contemporary, important focus for enhancing animal welfare. Jacketing cynomolgus monkeys has been traditionally considered an exclusionary criterion for social housing based on unsubstantiated concerns that study conduct or telemetry equipment...

Year Published: 2015Topics: Biological Sampling & Physiological Measurement, Social Housing & CompanionshipAnimal Type: Macaque, Nonhuman Primate

Citation: Kaiser, R. A., Tichenor, S. D., Regalia, D. E. et al. 2015. Telemetric assessment of social and single housing: Evaluation of electrocardiographic intervals in jacketed cynomolgus monkeys. Journal of Pharmacological and Toxicological Methods 75, 38-43.