Positive reinforcement training as a refinement of a macaque biliary diversion model (2003)

Friscino, B. H., Gai, C. L., Kulick, A. A. et al.

Abstract

Animals that adapted to wearing jackets were surgically implanted with a biliary diversion cannula system, a venous cannula and three subcutaneous access ports. .. The animals [three females and nine male rhesus] were trained to present the pouch and to remain stationary while the catheters were accessed. The length of time required for training was variable between individuals, but generally required three to four training sessions during a two-week period. These in-cage procedures precluded the need for chair or manual restraint of animals during sample collection. Instead, positive reinforcement was used to reward the animals with food for their cooperation during sample collection. This has also increased the efficiency of conducing metabolic studies and minimized the potential stress of sample collection for both the personnel and animals.