Implementing refinement in the EAE mouse model (2025)

Kuncova, S.

Abstract

Experimental autoimmune encephalomyelitis (EAE) is a model used in rodents to induce inflammation in the central nervous system. It is commonly used as a model for human multiple sclerosis (MS) and other diseases that involve demyelination. EAE has the potential to cause severe suffering in mice with muyltiple clinical signs such as ulcerated wounds after injections, tail paralysis and fore- and hind-limb paralysis. The welfare of the animals must be taken in o consideration and providing appropriate care is crucial for animal welfare and scientific outcomes. This poster provides an overview of refinements implemented at The Francis Crick Institute to minimise animal pain and discomfort.