Zebrafish

A rapid and noninvasive method that extracts polymerase chain reaction-ready genomic DNA from adult zebrafish

Genotyping usually entails analysis of the products of polymerase chain reaction (PCR) carried out with genomic DNA (gDNA) as template, and is employed for validation of mutant or transgenic organisms. For genotyping of adult zebrafish, gDNA is often extracted from...

Year Published: 2022Topics: Identification MethodAnimal Type: Fish, Zebrafish

Citation: Jang, J. Y., Liang, T., Kim, M.-K. et al. 2022. A rapid and noninvasive method that extracts polymerase chain reaction-ready genomic DNA from adult zebrafish. Zebrafish 19(4), 160-164.

Anesthesia overdose versus rapid cooling for euthanasia of adult zebrafish

The rapid increase in zebrafish use needs to be accompanied by research into the refinement of procedures. The European (EU) Directive lists three possible euthanasia methods for fish: anesthetic overdose, electrical stunning, and concussion. However, for small fish such as...

Year Published: 2022Topics: EuthanasiaAnimal Type: Fish, Zebrafish

Citation: Ferreira, J. M., Félix, L., Jorge, S. et al. 2022. Anesthesia overdose versus rapid cooling for euthanasia of adult zebrafish. Zebrafish 19(4), 148-159.

A psychological aversive condition does not change individual zebrafish preference for background color or artificial plant enrichments

Information on fish preference for environmental conditions can be a useful tool to offer them what they want, an important requirement for welfare purposes. Giving that such an approach deals with psychological states of the fish, we investigated whether psychological...

Year Published: 2022Topics: Environmental Enrichment, Housing, PersonalityAnimal Type: Fish, Zebrafish

Citation: Marques Maia, C., Volpato, G. L., Braithwaite, V. A. 2022. A psychological aversive condition does not change individual zebrafish preference for background color or artificial plant enrichments. Journal of Applied Animal Welfare Science 25(4), 427-438.

FELASA-AALAS recommendations for monitoring and reporting of laboratory fish diseases and health status, with an emphasis on zebrafish (Danio rerio)

The exchange of fish for research may expose an aquatic laboratory to pathogen contamination as incoming fish can introduce bacteria, fungi, parasites, and viruses capable of affecting both experimental results and fish and personnel health and welfare. To develop risk...

Year Published: 2022Topics: Husbandry & Management, Welfare AssessmentAnimal Type: Fish, Zebrafish

Citation: Mocho, J.-P., Collymore, C., Farmer, S. C. et al. 2022. FELASA-AALAS recommendations for monitoring and reporting of laboratory fish diseases and health status, with an emphasis on zebrafish (Danio rerio). Comparative Medicine 72(3), 127-148.

Internal temperatures of packaging for overnight cross-country shipping of zebrafish (Danio rerio)

As the use of zebrafish (Danio rerio) as a research model continues to rise, so too will the shipping and sharing of zebrafish strains across collaborating institutions. If done incorrectly, shipping can result in significant mortality, welfare concerns, and loss...

Year Published: 2022Topics: Relocation & TransportAnimal Type: Fish, Zebrafish

Citation: Fehrenbach, L. A., Adekanye, A. A., Palillo, M. B. et al. 2022. Internal temperatures of packaging for overnight cross-country shipping of zebrafish (Danio rerio). Comparative Medicine 72(3), 195-203.

Propofol immersion as a euthanasia method for adult zebrafish (Danio rerio)

The exponential rise of the zebrafish (Danio rerio) as a model organism in biomedical research has far outstripped our un- derstanding of basic husbandry and welfare for this species. As a case in point, here we investigate the efficacy and...

Year Published: 2022Topics: EuthanasiaAnimal Type: Fish, Zebrafish

Citation: Davis, A. K., Garner, J. P., Chu, D. K. et al. 2022. Propofol immersion as a euthanasia method for adult zebrafish (Danio rerio). Comparative Medicine 72(3), 204-209.

Non-invasive sampling of water-borne hormones demonstrates individual consistency of the cortisol response to stress in laboratory zebrafish (Danio rerio)

Glucocorticoid (GC) stress hormones are well-known for their impact on phenotypic traits ranging from immune function to behaviour and cognition. For that reason, consistent aspects of an individual's physiological stress response (i.e. GC responsiveness) can predict major elements of life-history...

Year Published: 2022Topics: Biological Sampling & Physiological MeasurementAnimal Type: Fish, Zebrafish

Citation: Midttun, H., Øverli, Ø., Tudorache, C. et al. 2022. Non-invasive sampling of water-borne hormones demonstrates individual consistency of the cortisol response to stress in laboratory zebrafish (Danio rerio). Scientific Reports 12(1) 6278.

The sound world of zebrafish: A critical review of hearing assessment

Zebrafish, like all fish species, use sound to learn about their environment. Thus, human-generated (anthropogenic) sound added to the environment has the potential to disrupt the detection of biologically relevant sounds, alter behavior, impact fitness, and produce stress and other...

Year Published: 2022Topics: Husbandry & Management, Natural BehaviorAnimal Type: Fish, Zebrafish

Citation: Popper, A. N., Sisneros, J. A. 2022. The sound world of zebrafish: A critical review of hearing assessment. Zebrafish 19(2), 37-48.

Gills just want to have fun: Can fish play games, just like us?

It is common to observe play in dogs, cats, and birds, but have we been ignoring play in one of the most common house pets of all… fish? Aquarium fish are often used as meditative decoration in family households, but...

Year Published: 2022Topics: Natural BehaviorAnimal Type: Fish, Other Fish, Salmon, Tilapia, Trout, Zebrafish

Citation: Eisenbeiser, S., Serbe-Kamp, É., Gage, G. J. et al. 2022. Gills just want to have fun: Can fish play games, just like us? Animals 12(13), 1684.

Efficacy of tricaine (MS-222) and hypothermia as anesthetic agents for blocking sensorimotor responses in larval zebrafish

Tricaine, or MS-222, is the most commonly used chemical anesthetic in zebrafish research. It is thought to act via blocking voltage-gated sodium channels, though its mechanism of action, particularly at the neuronal level, is not yet fully understood. Here, we...

Year Published: 2022Topics: Anesthesia & SedationAnimal Type: Fish, Zebrafish

Citation: Leyden, C., Brüggemann, T., Debinski, F. et al. 2022. Efficacy of tricaine (MS-222) and hypothermia as anesthetic agents for blocking sensorimotor responses in larval zebrafish. Frontiers in Veterinary Science 9, 864573.