Zebrafish

Sex segregation affects exploratory and social behaviors of zebrafish according to controlled housing conditions

Zebrafish has become an animal model in research and articles have established ideal conditions for their maintenance. However, little is known regarding the influence of gender and other cues on zebrafish behavior. Thus, here we analyzed the exploratory and social...

Year Published: 2020Topics: Environmental EnrichmentAnimal Type: Fish, Zebrafish

Citation: Mendonça Soares, S., Kirsten, K., Pompermaier, A. et al. 2020. Sex segregation affects exploratory and social behaviors of zebrafish according to controlled housing conditions. Physiology & Behavior 222, 112944.

Skin swabbing is a refined technique to collect DNA from model fish species

Model fish species such as sticklebacks and zebrafish are frequently used in studies that require DNA to be collected from live animals. This is typically achieved by fin clipping, a procedure that is simple and reliable to perform but that...

Year Published: 2020Topics: Identification MethodAnimal Type: Fish, Other Fish, Zebrafish

Citation: Tilley, C. A., Carreño Gutierrez, H., Sebire, M. et al. 2020. Skin swabbing is a refined technique to collect DNA from model fish species. Scientific Reports 10(1), 18212.

Best practices for non-lethal blood sampling of fish via the caudal vasculature

Blood sampling through the caudal vasculature is a widely used technique in fish biology for investigating organismal health and physiology. In live fishes, it can provide a quick, easy and relatively non‐invasive method for obtaining a blood sample (cf ....

Year Published: 2020Topics: Biological Sampling & Physiological MeasurementAnimal Type: Fish, Other Fish, Salmon, Tilapia, Trout, Zebrafish

Citation: Lawrence, M. J., Raby, G. D., Teffer, A. K. et al. 2020. Best practices for non-lethal blood sampling of fish via the caudal vasculature. Journal of Fish Biology 97(1), 4–15.

Collective behavior in wild zebrafish

Anthropogenic change is expected to alter environments at alarming rates. To predict the impact of modified environments on social behavior, we must study the relationship between environmental features and collective behavior in a genetically tractable model, zebrafish (Danio rerio). Here,...

Year Published: 2020Topics: Natural BehaviorAnimal Type: Fish, Zebrafish

Citation: Shelton, D. S., Shelton, S. G., Daniel, D. K. et al. 2020. Collective behavior in wild zebrafish. Zebrafish 17(4), 243-252.

Enrichment preferences of singly housed zebrafish (Danio rerio)

Despite the increasing popularity of zebrafish (Danio rerio) as an animal model, the environmental enrichment preferences of this species have been largely unexplored. We sought to determine the preferences of mature female zebrafish that were singly housed with or without...

Year Published: 2020Topics: Environmental EnrichmentAnimal Type: Fish, Zebrafish

Citation: Krueger, L. D., Thurston, S. E., Kirk, J. et al. 2020. Enrichment preferences of singly housed zebrafish (Danio rerio). JAALAS 59(2), 148–155.

Increase in environmental temperature affects exploratory behaviour, anxiety and social preference in Danio rerio

The aim of this work is to investigate the effect of a temperature increase on the behaviour of adult zebrafish (Danio rerio) maintained for 21 days at 34 °C (treatment) and 26 °C (control). The temperatures chosen are within the vital range...

Year Published: 2020Topics: Husbandry & ManagementAnimal Type: Fish, Zebrafish

Citation: Angiulli, E., Pagliara, V., Cioni, C. et al. 2020. Increase in environmental temperature affects exploratory behaviour, anxiety and social preference in Danio rerio. Scientific Reports 10(1), 5385.

Noninvasive multielectrode array for brain and spinal cord local field potential recordings from live zebrafish larvae

Zebrafish are an important and expanding experimental system for brain research. We describe a noninvasive electrophysiology technique that can be used in living larvae to measure spontaneous activity in the brain and spinal cord simultaneously. This easy-to-use method uses a...

Year Published: 2020Topics: Biological Sampling & Physiological MeasurementAnimal Type: Fish, Zebrafish

Citation: Tomasello, D. L., Sive, H. 2020. Noninvasive multielectrode array for brain and spinal cord local field potential recordings from live zebrafish larvae. Zebrafish 17(4), 271-277.

Methods of humane killing of laboratory fish: FELASA Working Group recommendations

As commissioned by the Federation of European Laboratory Animal Science Associations, these working group recommendations define the requirements to achieve the humane killing of fish, compare methods of killing, recommend methods of killing depending on context, and detail protocols leading...

Year Published: 2025Topics: EuthanasiaAnimal Type: Fish, Zebrafish

Citation: Mocho, J.-P., Blasco, J. R., Lundegaard, P. R. et al. 2025. Methods of humane killing of laboratory fish: FELASA Working Group recommendations. Laboratory Animals 59(5), 599–613.

Blue light preconditioning as a novel strategy for reducing transport stress in zebrafish

In recent years, zebrafish have become increasingly important in experimental sciences, with transportation being a critical step in their use. However, the vibrations during transportation often induce stress responses in the fish, impairing their physiological functions and even leading to...

Year Published: 2025Topics: Relocation & TransportAnimal Type: Fish, Zebrafish

Citation: Huo, L., Miao, X., Zhang, X. et al. 2025. Blue light preconditioning as a novel strategy for reducing transport stress in zebrafish. Behavioural Processes 231, 105260.

Preference for complex environments in larval zebrafish

Environmental complexity is increasingly recognized as a critical factor for animal welfare and cognitive development across vertebrates. While adult zebrafish (Danio rerio) show a strong preference for complex environments, little is known about the importance of environmental complexity in larval...

Year Published: 2025Topics: Environmental EnrichmentAnimal Type: Fish, Zebrafish

Citation: Gatto, E., Agrillo, C., Dadda, M. et al. 2025. Preference for complex environments in larval zebrafish. Applied Animal Behaviour Science 292, 106788.