Zebrafish

Methods of humane killing of laboratory fish: FELASA Working Group recommendations

As commissioned by the Federation of European Laboratory Animal Science Associations, these working group recommendations define the requirements to achieve the humane killing of fish, compare methods of killing, recommend methods of killing depending on context, and detail protocols leading...

Year Published: 2025Topics: EuthanasiaAnimal Type: Fish, Zebrafish

Citation: Mocho, J.-P., Blasco, J. R., Lundegaard, P. R. et al. 2025. Methods of humane killing of laboratory fish: FELASA Working Group recommendations. Laboratory Animals 59(5), 599–613.

Blue light preconditioning as a novel strategy for reducing transport stress in zebrafish

In recent years, zebrafish have become increasingly important in experimental sciences, with transportation being a critical step in their use. However, the vibrations during transportation often induce stress responses in the fish, impairing their physiological functions and even leading to...

Year Published: 2025Topics: Relocation & TransportAnimal Type: Fish, Zebrafish

Citation: Huo, L., Miao, X., Zhang, X. et al. 2025. Blue light preconditioning as a novel strategy for reducing transport stress in zebrafish. Behavioural Processes 231, 105260.

Preference for complex environments in larval zebrafish

Environmental complexity is increasingly recognized as a critical factor for animal welfare and cognitive development across vertebrates. While adult zebrafish (Danio rerio) show a strong preference for complex environments, little is known about the importance of environmental complexity in larval...

Year Published: 2025Topics: Environmental EnrichmentAnimal Type: Fish, Zebrafish

Citation: Gatto, E., Agrillo, C., Dadda, M. et al. 2025. Preference for complex environments in larval zebrafish. Applied Animal Behaviour Science 292, 106788.

The maintenance conditions of the zebrafish (Danio rerio) breeding stock affect the survival and behavior of their offspring

The environment in which we live influences our biological traits throughout our ontogeny, being markedly significant during the beginning of our lives. Some characteristics inherited (genetically and/or epigenetically) from parents can also influence individual development, known as transgenerational effects. In...

Year Published: 2025Topics: Environmental Enrichment, Husbandry & Management, ReproductionAnimal Type: Fish, Zebrafish

Citation: Amaral, F. U. I., Pompermaier, A., Rutikoski, G. et al. 2025. The maintenance conditions of the zebrafish (Danio rerio) breeding stock affect the survival and behavior of their offspring. Applied Animal Behaviour Science 292, 106761.

Editorial: Fish welfare in aquaculture and research—where are we going?

Rapid technical developments are taking place in aquaculture, and its production is increasing globally every year by more than 4%. Research is the necessary foundation for politics to define reasonable regulations and recommend guidelines for aquaculture, but also the basis...

Year Published: 2025Topics: Rearing & Weaning, Stocking Density, Welfare AssessmentAnimal Type: Fish, Other Fish, Salmon, Trout, Zebrafish

Citation: Pietsch, C. 2025. Editorial: Fish welfare in aquaculture and research—where are we going? Animals 15(16), 2367.

Evaluating the suitability of substrate enrichment for Zebrafish in high-capacity research aquaria

Zebrafish, Danio rerio, show a preference for substrate enrichment within their tanks however this is seldom included within high-capacity research aquaria due to fears its inclusion may impact on their ability to breed on demand. To investigate this potential impact...

Year Published: 2025Topics: Environmental Enrichment, Husbandry & Management, ReproductionAnimal Type: Fish, Zebrafish

Citation: McKay-West, J., Green, M., Chance, R. 2025. Evaluating the suitability of substrate enrichment for Zebrafish in high-capacity research aquaria. Animal Technology and Welfare 24(2), 108–112.

From Fish to Mice to Monkeys in Research: Challenges and Opportunities for Mental Well-being

This chapter focuses mainly on animals in medical research. Many, especially the smaller species such as rat, mice, and zebrafish, may be seen only a few minutes a day by their human caregivers. Most laboratory animals are kept in confinement...

Year Published: 2025Topics: Abnormal/Problematic Behavior, Emotion, Pain, & Sentience, Environmental Enrichment, Handling, Housing, Human-Animal Interaction, Husbandry & Management, Natural Behavior, Regulations & Ethical Review, Social Housing & CompanionshipAnimal Type: All/General, Fish, Macaque, Marmoset, Mouse, Nonhuman Primate, Rat, Rodent, Zebrafish

Citation: Serageldine, C. E., Robinson-Junker, A., Alvino, G. et al. 2025. From Fish to Mice to Monkeys in Research: Challenges and Opportunities for Mental Well-being. In Mental Health and Well-being in Animals (pp. 308–322).

Behavioral Biology of Laboratory Animals

This 30-chapter volume informs students and professionals about the behavioral biology of animals commonly housed in laboratory and other captive settings. Each species evolved under specific environmental conditions, resulting in unique behavioral patterns, many of which are maintained in captivity...

Year Published: 2022Topics: Abnormal/Problematic Behavior, Animal Training, Environmental Enrichment, Handling, Housing, Human-Animal Interaction, Husbandry & Management, Natural Behavior, Rearing & Weaning, Social Housing & Companionship, Welfare AssessmentAnimal Type: All/General, Amphibian, Baboon, Capuchin, Cat, Cattle, Chicken, Crocodile & Alligator, Dog, Equine, Ferret, Finch, Fish, Fowl, Frog & Toad, Gerbil, Guinea Pig, Hamster, Lizard, Macaque, Marmoset, Mouse, Nonhuman Primate, Other Rodent, Owl Monkey, Pig, Rabbit, Rat, Reptile, Rodent, Salamander, Sheep, Snake, Squirrel Monkey, Turtle & Tortoise, Vervet (African Green Monkey), Zebrafish

Citation: Coleman, K., Schapiro, S. J. (Eds.) 2022. Behavioral Biology of Laboratory Animals (1st Ed.). CRC Press, Boca Raton, FL, 560 p.

Housing and husbandry factors affecting zebrafish novel tank test responses: A global multi-laboratory study

The reproducibility crisis in bioscience, characterized by inconsistent study results, impedes our understanding of biological processes. Global collaborative studies offer a unique solution to this problem. Here, we present a global collaboration using the zebrafish (Danio rerio) novel tank test,...

Year Published: 2025Topics: Housing, Husbandry & ManagementAnimal Type: Fish, Zebrafish

Citation: Hillman, C., Fontana, B. D., Amstislavskaya, T. G. et al. 2025. Housing and husbandry factors affecting zebrafish novel tank test responses: A global multi-laboratory study. Lab Animal 54(6), 156–164.

An adaptable, user-friendly score sheet to monitor welfare in experimental fish

Fish are increasingly used as experimental animals across research fields. Currently, around a quarter of all experimental animals used are fish. Less than 20% of these are standard model species. Welfare assessments for experimental fish are in their infancy compared...

Year Published: 2025Topics: Welfare AssessmentAnimal Type: Fish, Other Fish, Salmon, Shark & Ray, Tilapia, Trout, Zebrafish

Citation: Flueck-Giraud, M., Schmidt-Posthaus, H., Bergadano, A. et al. 2025. An adaptable, user-friendly score sheet to monitor welfare in experimental fish. Laboratory Animals 59(2), 226–241.