Fish

Non-invasive sampling of water-borne hormones demonstrates individual consistency of the cortisol response to stress in laboratory zebrafish (Danio rerio)

Glucocorticoid (GC) stress hormones are well-known for their impact on phenotypic traits ranging from immune function to behaviour and cognition. For that reason, consistent aspects of an individual's physiological stress response (i.e. GC responsiveness) can predict major elements of life-history...

Year Published: 2022Topics: Biological Sampling & Physiological MeasurementAnimal Type: Fish, Zebrafish

Citation: Midttun, H., Øverli, Ø., Tudorache, C. et al. 2022. Non-invasive sampling of water-borne hormones demonstrates individual consistency of the cortisol response to stress in laboratory zebrafish (Danio rerio). Scientific Reports 12(1) 6278.

The sound world of zebrafish: A critical review of hearing assessment

Zebrafish, like all fish species, use sound to learn about their environment. Thus, human-generated (anthropogenic) sound added to the environment has the potential to disrupt the detection of biologically relevant sounds, alter behavior, impact fitness, and produce stress and other...

Year Published: 2022Topics: Husbandry & Management, Natural BehaviorAnimal Type: Fish, Zebrafish

Citation: Popper, A. N., Sisneros, J. A. 2022. The sound world of zebrafish: A critical review of hearing assessment. Zebrafish 19(2), 37-48.

Evaluation of prolonged immersion in tricaine methanesulfonate for juvenile goldfish (Carassius auratus) euthanasia

OBJECTIVE: Doses of buffered tricaine methanesulfonate (MS-222) up to 1000 mg/L for 15 minutes are reported inefficient to produce euthanasia in goldfish. The goal of this study was to determine if goldfish can be euthanized by more prolonged immersion in...

Year Published: 2022Topics: EuthanasiaAnimal Type: Fish, Other Fish

Citation: Perret-Thiry, C., Raulic, J., Vergneau-Grosset, C. 2022. Evaluation of prolonged immersion in tricaine methanesulfonate for juvenile goldfish (Carassius auratus) euthanasia. Journal of the American Veterinary Medical Association 260(8), 911-915.

Non-lethal sampling for the stable isotope analysis of the critically endangered European eel Anguilla anguilla: How fin and mucus compare to dorsal muscle

Ecological studies on the critically endangered European eel Anguilla anguilla often incorporate stable isotope analysis that typically uses dorsal muscle sampled from euthanised eels. To minimise the lethal sampling of imperilled populations, fin tissue and/or epidermal mucus can provide non-lethal...

Year Published: 2022Topics: Biological Sampling & Physiological MeasurementAnimal Type: Fish, Other Fish

Citation: Boardman, R. M., Pinder, A. C., Piper,A. T. et al. 2022. Non-lethal sampling for the stable isotope analysis of the critically endangered European eel Anguilla anguilla: How fin and mucus compare to dorsal muscle. Journal of Fish Biology 100(3), 847-851.

Efficacy of tricaine (MS-222) and hypothermia as anesthetic agents for blocking sensorimotor responses in larval zebrafish

Tricaine, or MS-222, is the most commonly used chemical anesthetic in zebrafish research. It is thought to act via blocking voltage-gated sodium channels, though its mechanism of action, particularly at the neuronal level, is not yet fully understood. Here, we...

Year Published: 2022Topics: Anesthesia & SedationAnimal Type: Fish, Zebrafish

Citation: Leyden, C., Brüggemann, T., Debinski, F. et al. 2022. Efficacy of tricaine (MS-222) and hypothermia as anesthetic agents for blocking sensorimotor responses in larval zebrafish. Frontiers in Veterinary Science 9, 864573.

Effects of environmental enrichment on recognition memory in zebrafish larvae

Zebrafish (Danio rerio) constitute a useful model for studying memory function and impairment in vertebrates and are now widely used in translational research. On the one hand, the adoption of simple, fast and reliable tests such as novel object recognition...

Year Published: 2022Topics: Environmental EnrichmentAnimal Type: Fish, Zebrafish

Citation: Gatto, E., Bruzzone, M., Dal Maschio, M. et al. 2022. Effects of environmental enrichment on recognition memory in zebrafish larvae. Applied Animal Behaviour Science 247, 105552.

The effect of environmental enrichment on laboratory rare minnows (Gobiocypris rarus): Growth, physiology, and behavior

Environmental enrichment is a method to increase environmental heterogeneity, which may reduce stress and improve animal welfare. Previous studies have shown that environmental enrichment can increase the growth rate, decrease aggressive and anxiety-like behaviors, improve learning ability and agility, and...

Year Published: 2022Topics: Environmental EnrichmentAnimal Type: Fish, Other Fish

Citation: Xu, C., Hou, M., Su, L. et al. 2022. The effect of environmental enrichment on laboratory rare minnows (Gobiocypris rarus): Growth, physiology, and behavior. Animals 12(4), 514.

Welfare assessment of adult laboratory zebrafish: A practical guide

Teleost fish such as Danio rerio (zebrafish) have been successfully used in biomedical research since decades. Genetically altered fish lines obtained by state-of-the-art genetic technologies are serving as well-known model organisms. In Europe, following Directive 2010/63/EU, generation, breeding, and husbandry...

Year Published: 2021Topics: Welfare AssessmentAnimal Type: Fish, Zebrafish

Citation: Sabrautzki, S., Miller, M., Kague, E. et al. 2021. Welfare assessment of adult laboratory zebrafish: A practical guide. Zebrafish 18(4), 282-292.

Effects of holding density on the welfare of zebrafish: A systematic review

The zebrafish is becoming an increasingly popular research animal around the world. Its welfare is affected by an array of environmental factors, such as food access and water quality. Holding density is an important welfare aspect, not least due to...

Year Published: 2021Topics: Stocking DensityAnimal Type: Fish, Zebrafish

Citation: Andersson, M., Kettunen, P. 2021. Effects of holding density on the welfare of zebrafish: A systematic review. Zebrafish 18(5), 297-306.

Efficacy and behavioral recovery of different anesthetics

The use of proper anesthesia in zebrafish research is essential to ensure fish welfare and data reliability. However, anesthesia long-term side effects remain poorly understood. The purpose of this study was to assess anesthesia quality and recovery in adult zebrafish...

Year Published: 2021Topics: Anesthesia & SedationAnimal Type: Fish, Zebrafish

Citation: Jorge, S., Ferreira, J. M., Olsson, I. A. S. et al. 2021. Efficacy and behavioral recovery of different anesthetics. Zebrafish 18(5), 330-337.